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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
13/08/2014 |
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Data da última atualização: |
25/09/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
RODRÍGUEZ OSUNA, V.; BÖRNER, J.; NEHREN, U.; PRADO, R. B.; GAESE, H.; HEINRICH, J. |
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Afiliação: |
Vanesa Rodríguez Osuna, Department of Economic and Technological; Jan Börner, Department of Economic and Technological Change; Udo Nehren, Institute for Technology and Resources Management in the Tropics and Subtropics; RACHEL BARDY PRADO, CNPS; Hartmut Gaese, Department of Economic and Technological Change; Jürgen Heinrich, Institute for Geography, Department of Physical Geography, University of Leipzig. |
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Título: |
Priority areas for watershed service conservation in the Guapi-Macacu region of Rio de Janeiro, Atlantic Forest, Brazil. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Ecological Processes, v. 3, n. 16, 2014. |
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DOI: |
10.1186/s13717-014-0016-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction: Land use intensification and urbanisation processes are degrading hydrological ecosystem services in the Guapi-Macacu watershed of Rio de Janeiro. A proposal to pay farmers to restore natural watershed services might be an alternative to securing the water supply in the long-term for the around 2.5 million urban water users in the study region. This study quantifies the costs of changing current land use patterns to enhance watershed services and compares these costs to the avoided costs associated with water treatment for public supply. Methods: We use farm-household data to estimate the opportunity costs of abandoning current land uses for the recovery of natural vegetation; a process that is very likely to improve water quality in terms of turbidity due to reduced inputs from erosion. Opportunity cost estimates are extrapolated to the watershed scale based on remote sensing land use classifications and vulnerability analysis to identify priority zones for watershed management interventions. To assess the potential demand for watershed services, we analyse water quality and treatment cost data from the main local water treatment plant. Results: Changing agricultural land uses for watershed services provision generally comes at high opportunity costs in our study area near to the metropolis of Rio de Janeiro. Alternative low cost watershed conservation options do exist in the livestock production sector. These options have the potential to directly reduce the amount of sediments and nutrients reaching the water bodies, and in turn decrease the costs of treatment needed for drinking water. Land cover changes at the scale needed to improve water quality will, nonetheless, likely exceed the cost of additional investments in water treatment. Conclusions: The state water utility company?s willingness to pay for watershed services alone will not be enough to induce provision of additional watershed services. We conclude that monetary incentives conditioned on specific adjustments to existing production systems could still have a complementary role to play in improving watershed services. However, we note that our willingness to pay analysis focusses on only one of the potentially wide range of ecosystem services provided by natural vegetation in the Guapi-Macacu watershed. Factoring these ecosystem services into the willingness to pay equation is likely to change our assessment in favour of additional conservation action, be it through PES or other policy instruments. MenosIntroduction: Land use intensification and urbanisation processes are degrading hydrological ecosystem services in the Guapi-Macacu watershed of Rio de Janeiro. A proposal to pay farmers to restore natural watershed services might be an alternative to securing the water supply in the long-term for the around 2.5 million urban water users in the study region. This study quantifies the costs of changing current land use patterns to enhance watershed services and compares these costs to the avoided costs associated with water treatment for public supply. Methods: We use farm-household data to estimate the opportunity costs of abandoning current land uses for the recovery of natural vegetation; a process that is very likely to improve water quality in terms of turbidity due to reduced inputs from erosion. Opportunity cost estimates are extrapolated to the watershed scale based on remote sensing land use classifications and vulnerability analysis to identify priority zones for watershed management interventions. To assess the potential demand for watershed services, we analyse water quality and treatment cost data from the main local water treatment plant. Results: Changing agricultural land uses for watershed services provision generally comes at high opportunity costs in our study area near to the metropolis of Rio de Janeiro. Alternative low cost watershed conservation options do exist in the livestock production sector. These options have the potential to directly reduce the ... Mostrar Tudo |
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Palavras-Chave: |
Drinking water treatment; Gestão de bacias hidrográficas baseada em incentivos; Incentive-based watershed managemenT; Serviços de água; Tratamento de água potável; Water services. |
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Thesagro: |
Qualidade da Água. |
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Thesaurus Nal: |
water quality. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/106452/1/s13717-014-0016-7.pdf
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Marc: |
LEADER 03462naa a2200289 a 4500
001 1992454
005 2019-09-25
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1186/s13717-014-0016-7$2DOI
100 1 $aRODRÍGUEZ OSUNA, V.
245 $aPriority areas for watershed service conservation in the Guapi-Macacu region of Rio de Janeiro, Atlantic Forest, Brazil.$h[electronic resource]
260 $c2014
520 $aIntroduction: Land use intensification and urbanisation processes are degrading hydrological ecosystem services in the Guapi-Macacu watershed of Rio de Janeiro. A proposal to pay farmers to restore natural watershed services might be an alternative to securing the water supply in the long-term for the around 2.5 million urban water users in the study region. This study quantifies the costs of changing current land use patterns to enhance watershed services and compares these costs to the avoided costs associated with water treatment for public supply. Methods: We use farm-household data to estimate the opportunity costs of abandoning current land uses for the recovery of natural vegetation; a process that is very likely to improve water quality in terms of turbidity due to reduced inputs from erosion. Opportunity cost estimates are extrapolated to the watershed scale based on remote sensing land use classifications and vulnerability analysis to identify priority zones for watershed management interventions. To assess the potential demand for watershed services, we analyse water quality and treatment cost data from the main local water treatment plant. Results: Changing agricultural land uses for watershed services provision generally comes at high opportunity costs in our study area near to the metropolis of Rio de Janeiro. Alternative low cost watershed conservation options do exist in the livestock production sector. These options have the potential to directly reduce the amount of sediments and nutrients reaching the water bodies, and in turn decrease the costs of treatment needed for drinking water. Land cover changes at the scale needed to improve water quality will, nonetheless, likely exceed the cost of additional investments in water treatment. Conclusions: The state water utility company?s willingness to pay for watershed services alone will not be enough to induce provision of additional watershed services. We conclude that monetary incentives conditioned on specific adjustments to existing production systems could still have a complementary role to play in improving watershed services. However, we note that our willingness to pay analysis focusses on only one of the potentially wide range of ecosystem services provided by natural vegetation in the Guapi-Macacu watershed. Factoring these ecosystem services into the willingness to pay equation is likely to change our assessment in favour of additional conservation action, be it through PES or other policy instruments.
650 $awater quality
650 $aQualidade da Água
653 $aDrinking water treatment
653 $aGestão de bacias hidrográficas baseada em incentivos
653 $aIncentive-based watershed managemenT
653 $aServiços de água
653 $aTratamento de água potável
653 $aWater services
700 1 $aBÖRNER, J.
700 1 $aNEHREN, U.
700 1 $aPRADO, R. B.
700 1 $aGAESE, H.
700 1 $aHEINRICH, J.
773 $tEcological Processes$gv. 3, n. 16, 2014.
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Registro original: |
Embrapa Solos (CNPS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
05/09/2016 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVARES, C. C. S.; ALFRADIQUE, V. A. P.; SOUZA FABJAN, J. M. G.; FONSECA, J. F. da; SARAIVA, H. F. R. A.; CÔRTES, L. R.; COSTA, L. C. da; BRANDÃO, F. Z. |
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Afiliação: |
Universidade Federal Fluminense (UFF) - Niteroi, RJ; UFF - Niteroi, RJ; UFF - Niteroi, RJ; JEFERSON FERREIRA DA FONSECA, CNPC; UFF - Niteroi, RJ; UFF - Niteroi, RJ; UFF - Niteroi, RJ; UFF - Niteroi, RJ. |
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Título: |
Influence of mini-Percoll techniques in sperm capacitation and plasma membrane integrity of ram frozen-thawed sperm. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Animal Reproduction, v. 13, n. 3, p. 552, Jul./Sept. 2016. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016. |
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Conteúdo: |
Abstract: The success of IVF and subsequent development of embryos are directly related to sperm selection and quality. This study aimed to evaluate the effect of different forces and time of centrifugation in mini-Percoll (MP) techniques for sperm selection on capacitation status and plasma membrane (PM) integrity of ram frozen-thawed sperm. Commercial semen from 10 rams of Santa Inês breed, aging 2-5 years old, were used. At post-thawing (PT), the PM integrity was evaluated by CASA using the SCA® system (Sperm Class Analyzer ? Microptic Automatic Diagnostic Systems, Barcelona, Spain). For traditional Percoll (Sigma Chemical, St. Louis, USA), a 2 mL-gradient (90/45% density) was subjected to a 700 x g centrifugation for 10 min followed by 200 x g for 5 min. The MP techniques consisted in an 800 µL-gradient (90/45% density) subjected to either: I) two centrifugations of 5000 x g for 5 min; II) two centrifugations of 2500 x g for 5 min; III) two centrifugations of 1250 x g for 5 min; IV) 700 x g for 10 min, followed by 200 x g for 5 min. At the end of all treatments, aliquots (post-protocols = 0 h) were taken for evaluation of PM integrity and capacitation status by chlortetracycline stain (Sigma Chemical, St. Louis, USA). Later on, samples of all treatments were submitted to incubation at 37°C, 1 h, 2 h and 3 h and the same parameters were assessed. The variables were subjected to either ANOVA or Kruskal-Wallis tests depending on normality, Tukey and Fisher-LSD analysis (P < 0.05). There was no difference (P > 0.05) among all treatments for capacitation status and PM integrity. The capacitated rate was higher (P < 0.05) at 3 h (28.6 ± 2.5%) when compared with 0 h (23.3 ± 1.7%) and 1 h (23.8 ± 1.4%) of incubation, regardless of the treatment. There was no difference (P > 0.05) for PM integrity among the protocols at 0h. Regardless of the treatment, the intact and damaged PM rate, respectively, were similar (P > 0.05) for PT (16.4 ± 2.0% vs. 84.0 ± 2.0%) and 0 h (19.0 ± 2.9% vs. 81.1 ± 2.9%; average of all treatments). When analyzing just intact cells, the PT values were greater (P < 0.05) than any incubation interval, whereas the latter were lower than 0 h values (P < 0.05). In conclusion, MP may be used as an ideal alternative to the traditional Percoll, decreasing costs and time of sperm handling, without cell damages in ram sperm. Chemical, St. Louis, USA). [Influência de diferentes técnicas de mini-percoll na capacitação espermática e integridade de membrana plasmática de sêmen descongelado de carneiros]. Resumo: O sucesso da FIV e posterior desenvolvimento embrionário são diretamente relacionados com a qualidade seminal e seleção espermática. O presente estudo teve como objetivo avaliar o efeito de diferentes forças e tempo de centrifugação de diferentes técnicas de seleção espermática de mini-Percoll (MP) no status da capacitação espermática e integridade da membrana plasmática (MP) de sêmen descongelado de carneiros. Foram utilizadas 10 palhetas de sêmen comercial congelado de diferentes carneiros da raça Santa Inês, com idade entre 2-5 anos. Na avaliação pós descongelamento (PD), a integridade de MP foi analisada pelo CASA por meio do sistema SCA® (Sperm Class Analyzer ? Microptic Automatic Diagnostic Systems, Barcelona, Espanha). A técnica de Percoll (Sigma Chemical, St. Louis, USA) tradicional consistiu em utilizar 2 mL de gradiente (90/45%) e centrifugar a uma força de 700 x g por 10 minutos, seguida de outra centrifugação a 200 x g por 5 minutos. As técnicas de MP consistiram em utilizar um volume de 800 ?L de gradiente (90/45%) que foram submetidos aos seguintes tempos e forças de centrifugação: I) duas centrifugações de 5000 x g por 5 minutos; II) duas centrifugações de 2500 x g por 5 minutos; III) duas centrifugações de1250 x g por 5 minutos; IV) 700 x g por 10 minutos, seguidas de outra centrifugação a 200 x g por 5 minutos. No fim dos tratamentos (pós protocolos = 0 h), alíquotas foram retiradas para avaliação da integridade de MP e status da capacitação espermática pela coloração de clortetraciclina (Sigma Chemical, St. Louis, USA). Posteriormente, as amostras de todos os tratamentos foram incubadas a 37 °C, 1 h, 2 h e 3 h e os mesmos parâmetros foram avaliados. As variáveis foram submetidas às análises de ANOVA ou teste de Kruskal-Wallis dependendo da normalidade, e testes de Tukey e Fisher-LSD (P < 0,05). Não houve diferença (P > 0,05) entre os tratamentos durante a avaliação do status da capacitação espermática e da integridade de MP. A taxa de capacitação foi maior (P < 0.05) em 3 h de incubação (28,6 ± 2,5%) quando comparado com 0 h (23,3 ± 1,7%) e 1 h (23,8 ± 1,4%), independente do tratamento utilizado. Não houve diferença (P > 0,05) em relação à integridade de MP entre os tratamentos a 0 h. Independente do tratamento utilizado, as taxas de MP íntegra e lesada, respectivamente, foram semelhantes (P > 0,05) no PD (16,4 ± 2,0% vs. 84,0 ± 2,0%; média de todos os tratamentos) e no momento 0 h (19,0 ± 2,9% vs. 81,1 ± 2,9%; média de todos os tratamentos). Quando foram analisadas somente células íntegras, os valores no PD foram maiores (P < 0,05) do que todos os intervalos de incubação, enquanto que todos os intervalos de incubação apresentaram taxas menores que os valores encontrados no momento 0 h (P < 0,05). Em conclusão, as técnicas de MP podem ser utilizadas como uma alternativa ideal ao método tradicional de Percoll durante a seleção espermática de sêmen congelado de carneiros, diminuindo custos e tempo de preparação espermática, sem que haja danos aos espermatozoides. MenosAbstract: The success of IVF and subsequent development of embryos are directly related to sperm selection and quality. This study aimed to evaluate the effect of different forces and time of centrifugation in mini-Percoll (MP) techniques for sperm selection on capacitation status and plasma membrane (PM) integrity of ram frozen-thawed sperm. Commercial semen from 10 rams of Santa Inês breed, aging 2-5 years old, were used. At post-thawing (PT), the PM integrity was evaluated by CASA using the SCA® system (Sperm Class Analyzer ? Microptic Automatic Diagnostic Systems, Barcelona, Spain). For traditional Percoll (Sigma Chemical, St. Louis, USA), a 2 mL-gradient (90/45% density) was subjected to a 700 x g centrifugation for 10 min followed by 200 x g for 5 min. The MP techniques consisted in an 800 µL-gradient (90/45% density) subjected to either: I) two centrifugations of 5000 x g for 5 min; II) two centrifugations of 2500 x g for 5 min; III) two centrifugations of 1250 x g for 5 min; IV) 700 x g for 10 min, followed by 200 x g for 5 min. At the end of all treatments, aliquots (post-protocols = 0 h) were taken for evaluation of PM integrity and capacitation status by chlortetracycline stain (Sigma Chemical, St. Louis, USA). Later on, samples of all treatments were submitted to incubation at 37°C, 1 h, 2 h and 3 h and the same parameters were assessed. The variables were subjected to either ANOVA or Kruskal-Wallis tests depending on normality, Tukey and Fisher-LSD analysis (P <... Mostrar Tudo |
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Palavras-Chave: |
Mini-Percoll; Sperm preparation; Sperm selection. |
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Thesagro: |
Espermatozóide; Ovelha; Ovino; Reprodução animal; Sêmen. |
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Thesaurus NAL: |
Sheep; Spermatozoa. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/147097/1/cnpc-2016-Influence-of.pdf
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Marc: |
LEADER 06949nam a2200325 a 4500
001 2052416
005 2019-09-23
008 2016 bl uuuu u00u1 u #d
100 1 $aOLIVARES, C. C. S.
245 $aInfluence of mini-Percoll techniques in sperm capacitation and plasma membrane integrity of ram frozen-thawed sperm.$h[electronic resource]
260 $aAnimal Reproduction, v. 13, n. 3, p. 552, Jul./Sept. 2016.$c2016
500 $aProceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain, September 9th and 10th, 2016.
520 $aAbstract: The success of IVF and subsequent development of embryos are directly related to sperm selection and quality. This study aimed to evaluate the effect of different forces and time of centrifugation in mini-Percoll (MP) techniques for sperm selection on capacitation status and plasma membrane (PM) integrity of ram frozen-thawed sperm. Commercial semen from 10 rams of Santa Inês breed, aging 2-5 years old, were used. At post-thawing (PT), the PM integrity was evaluated by CASA using the SCA® system (Sperm Class Analyzer ? Microptic Automatic Diagnostic Systems, Barcelona, Spain). For traditional Percoll (Sigma Chemical, St. Louis, USA), a 2 mL-gradient (90/45% density) was subjected to a 700 x g centrifugation for 10 min followed by 200 x g for 5 min. The MP techniques consisted in an 800 µL-gradient (90/45% density) subjected to either: I) two centrifugations of 5000 x g for 5 min; II) two centrifugations of 2500 x g for 5 min; III) two centrifugations of 1250 x g for 5 min; IV) 700 x g for 10 min, followed by 200 x g for 5 min. At the end of all treatments, aliquots (post-protocols = 0 h) were taken for evaluation of PM integrity and capacitation status by chlortetracycline stain (Sigma Chemical, St. Louis, USA). Later on, samples of all treatments were submitted to incubation at 37°C, 1 h, 2 h and 3 h and the same parameters were assessed. The variables were subjected to either ANOVA or Kruskal-Wallis tests depending on normality, Tukey and Fisher-LSD analysis (P < 0.05). There was no difference (P > 0.05) among all treatments for capacitation status and PM integrity. The capacitated rate was higher (P < 0.05) at 3 h (28.6 ± 2.5%) when compared with 0 h (23.3 ± 1.7%) and 1 h (23.8 ± 1.4%) of incubation, regardless of the treatment. There was no difference (P > 0.05) for PM integrity among the protocols at 0h. Regardless of the treatment, the intact and damaged PM rate, respectively, were similar (P > 0.05) for PT (16.4 ± 2.0% vs. 84.0 ± 2.0%) and 0 h (19.0 ± 2.9% vs. 81.1 ± 2.9%; average of all treatments). When analyzing just intact cells, the PT values were greater (P < 0.05) than any incubation interval, whereas the latter were lower than 0 h values (P < 0.05). In conclusion, MP may be used as an ideal alternative to the traditional Percoll, decreasing costs and time of sperm handling, without cell damages in ram sperm. Chemical, St. Louis, USA). [Influência de diferentes técnicas de mini-percoll na capacitação espermática e integridade de membrana plasmática de sêmen descongelado de carneiros]. Resumo: O sucesso da FIV e posterior desenvolvimento embrionário são diretamente relacionados com a qualidade seminal e seleção espermática. O presente estudo teve como objetivo avaliar o efeito de diferentes forças e tempo de centrifugação de diferentes técnicas de seleção espermática de mini-Percoll (MP) no status da capacitação espermática e integridade da membrana plasmática (MP) de sêmen descongelado de carneiros. Foram utilizadas 10 palhetas de sêmen comercial congelado de diferentes carneiros da raça Santa Inês, com idade entre 2-5 anos. Na avaliação pós descongelamento (PD), a integridade de MP foi analisada pelo CASA por meio do sistema SCA® (Sperm Class Analyzer ? Microptic Automatic Diagnostic Systems, Barcelona, Espanha). A técnica de Percoll (Sigma Chemical, St. Louis, USA) tradicional consistiu em utilizar 2 mL de gradiente (90/45%) e centrifugar a uma força de 700 x g por 10 minutos, seguida de outra centrifugação a 200 x g por 5 minutos. As técnicas de MP consistiram em utilizar um volume de 800 ?L de gradiente (90/45%) que foram submetidos aos seguintes tempos e forças de centrifugação: I) duas centrifugações de 5000 x g por 5 minutos; II) duas centrifugações de 2500 x g por 5 minutos; III) duas centrifugações de1250 x g por 5 minutos; IV) 700 x g por 10 minutos, seguidas de outra centrifugação a 200 x g por 5 minutos. No fim dos tratamentos (pós protocolos = 0 h), alíquotas foram retiradas para avaliação da integridade de MP e status da capacitação espermática pela coloração de clortetraciclina (Sigma Chemical, St. Louis, USA). Posteriormente, as amostras de todos os tratamentos foram incubadas a 37 °C, 1 h, 2 h e 3 h e os mesmos parâmetros foram avaliados. As variáveis foram submetidas às análises de ANOVA ou teste de Kruskal-Wallis dependendo da normalidade, e testes de Tukey e Fisher-LSD (P < 0,05). Não houve diferença (P > 0,05) entre os tratamentos durante a avaliação do status da capacitação espermática e da integridade de MP. A taxa de capacitação foi maior (P < 0.05) em 3 h de incubação (28,6 ± 2,5%) quando comparado com 0 h (23,3 ± 1,7%) e 1 h (23,8 ± 1,4%), independente do tratamento utilizado. Não houve diferença (P > 0,05) em relação à integridade de MP entre os tratamentos a 0 h. Independente do tratamento utilizado, as taxas de MP íntegra e lesada, respectivamente, foram semelhantes (P > 0,05) no PD (16,4 ± 2,0% vs. 84,0 ± 2,0%; média de todos os tratamentos) e no momento 0 h (19,0 ± 2,9% vs. 81,1 ± 2,9%; média de todos os tratamentos). Quando foram analisadas somente células íntegras, os valores no PD foram maiores (P < 0,05) do que todos os intervalos de incubação, enquanto que todos os intervalos de incubação apresentaram taxas menores que os valores encontrados no momento 0 h (P < 0,05). Em conclusão, as técnicas de MP podem ser utilizadas como uma alternativa ideal ao método tradicional de Percoll durante a seleção espermática de sêmen congelado de carneiros, diminuindo custos e tempo de preparação espermática, sem que haja danos aos espermatozoides.
650 $aSheep
650 $aSpermatozoa
650 $aEspermatozóide
650 $aOvelha
650 $aOvino
650 $aReprodução animal
650 $aSêmen
653 $aMini-Percoll
653 $aSperm preparation
653 $aSperm selection
700 1 $aALFRADIQUE, V. A. P.
700 1 $aSOUZA FABJAN, J. M. G.
700 1 $aFONSECA, J. F. da
700 1 $aSARAIVA, H. F. R. A.
700 1 $aCÔRTES, L. R.
700 1 $aCOSTA, L. C. da
700 1 $aBRANDÃO, F. Z.
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