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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
06/11/1995 |
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Data da última atualização: |
17/05/2023 |
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Autoria: |
ANTHONY, P.; DAVEY, M. R.; POWER, J. B.; LOWE, K. C. |
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Afiliação: |
University of Nottingham, university Park. |
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Título: |
An improved protocol for the culture of cassava leaf protoplasts. |
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Ano de publicação: |
1995 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, v.42, n.3, p.299-302, 1995. |
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ISSN: |
0167-6857 |
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Idioma: |
Inglês |
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Conteúdo: |
viable protoplasts (yield > 1.9 x 10 g-1 fresh weight; mean viability 85 + or - 2%, n=5) were isolated from leaves of axenic shoot cultures of Manihot esculenta Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free Ms medium. overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and protruding from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplast division was observed only in the presence of glass rods, where the initial planting efficiency was almost 6-fold greater than control (p< 0.05). The mean final plating efficiency of treated cultures was 1.0 +or - 0.2% while, in contrast, significant colony formation was not observed in controls. |
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Palavras-Chave: |
Glass rods; Plantaing efficiency; Two-phase culture system. |
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Thesagro: |
Manihot Esculenta. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01418naa a2200217 a 4500
001 1634106
005 2023-05-17
008 1995 bl uuuu u00u1 u #d
022 $a0167-6857
100 1 $aANTHONY, P.
245 $aAn improved protocol for the culture of cassava leaf protoplasts.$h[electronic resource]
260 $c1995
520 $aviable protoplasts (yield > 1.9 x 10 g-1 fresh weight; mean viability 85 + or - 2%, n=5) were isolated from leaves of axenic shoot cultures of Manihot esculenta Crantz. cv. M. Thai 8. Protoplasts were cultured for up to 50 days in liquid, ammonium-free Ms medium. overlaying agarose-solidified B5 medium with short glass rods embedded perpendicularly within, and protruding from, the agarose layer. Control protoplasts were cultured identically, but without glass rods. Sustained protoplast division was observed only in the presence of glass rods, where the initial planting efficiency was almost 6-fold greater than control (p< 0.05). The mean final plating efficiency of treated cultures was 1.0 +or - 0.2% while, in contrast, significant colony formation was not observed in controls.
650 $aManihot Esculenta
653 $aGlass rods
653 $aPlantaing efficiency
653 $aTwo-phase culture system
700 1 $aDAVEY, M. R.
700 1 $aPOWER, J. B.
700 1 $aLOWE, K. C.
773 $tPlant Cell, Tissue and Organ Culture$gv.42, n.3, p.299-302, 1995.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 2 | |
| 1. |  | RECH, E. L.; OCHATT, S. J.; CHAND, P. K.; POWER, J. B.; DAVEY, M. R. Electroenhancement of division of plant protoplast-derived cells. Protoplasma, New York, v.141, n.2/3, p.169-176, 1987. p.169-176| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | RECH, E. L.; OCHATT, S. J.; CHAND, P. K.; DAVEY, M. R.; MULLIGAN, B. J.; POWER, J. B. Electroporation increases DNA synthesis in cultured plant protoplasts. Biotechnology, New York, v.6. n.9, p.1091-1093, 1988.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 2 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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