| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
10/10/2013 |
|
Data da última atualização: |
09/03/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BEVITORI, R.; POPIELARSKA-KONIECZNA, M.; SANTOS, E. M. dos; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
|
Afiliação: |
ROSANGELA BEVITORI, CNPAF; M. POPIELARSKA-KONIECZNA, JAGIELLONIAN UNIVERSITY, CRACOW-POLAND; E. M. DOS SANTOS, UFG; MARIA FATIMA GROSSI DE SA, CENARGEN; S. PETROFEZA, UFG. |
|
Título: |
Morpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Protoplasma, New York, v. 251, n. 3, p. 545-554, May 2014. |
|
DOI: |
10.1007/s00709-013-0553-4 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation. |
|
Thesagro: |
Arroz; Embriogenese; Morfogenese; Oryza sativa. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179857/1/Bevitori2014-Article-Morpho-anatomicalCharacterizat.pdf
|
|
Marc: |
LEADER 02199naa a2200229 a 4500
001 1975223
005 2023-03-09
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s00709-013-0553-4$2DOI
100 1 $aBEVITORI, R.
245 $aMorpho-anatomical characterization of mature embryo-derived callus of rice (Oryza sativa L.) suitable for transformation.$h[electronic resource]
260 $c2014
520 $aThe objective of this study was to morphoanatomically characterize embryogenic rice calli during early induction of somatic embryogenesis of three Brazilian rice cultivars. Herein, we explored embryogenic units (EUs) from 2-week-old cut proliferated calli to verify whether they were suitable for Agrobacterium tumefasciens-mediated transformation. Histological analysis and scanning electron microscopy (SEM) were used to analyze these types of calli during early rice callogenesis in the cultivars BRS Primavera, BRS Bonança, and BRS Caiapó. The characteristics of the embryogenic cells were preserved in the EUs, which showed a globular, compact structure that contained tightly packed cells and thus rendered the cells suitable for transformation. The EUs of BRS Caiapó also maintained the characteristics of the nonembryogenic callus, such as an elongated morphology and a lack of cellular organization. In general, the observations of the histological sections corresponded with those of the SEM images. The histological analysis suggested that all cultivars used in these experiments have morphogenic potential. The EUs from proliferated 2-week-old cut calli maintained their embryogenic features. The EUs were subjected to Agrobacterium-mediated transformation, which exhibited a regeneration frequency of 58 % for transformed hygromycin-resistant cell lines. These results show that EUs from proliferated 2-week-old cut calli are suitable for plant transformation.
650 $aArroz
650 $aEmbriogenese
650 $aMorfogenese
650 $aOryza sativa
700 1 $aPOPIELARSKA-KONIECZNA, M.
700 1 $aSANTOS, E. M. dos
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tProtoplasma, New York$gv. 251, n. 3, p. 545-554, May 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agroenergia. |
|
Data corrente: |
06/12/2017 |
|
Data da última atualização: |
12/01/2018 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
STEFANINI, R.; PACHECO, T. F.; GONCALVES, S. B.; MACHADO, F. |
|
Afiliação: |
THALYTA FRAGA PACHECO, CNPAE; SILVIA BELEM GONCALVES, CNPAE. |
|
Título: |
M44: a factorial design analysis of growth kinetics of Pichia stipitis. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
In: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 39., 2017, São Francisco, CA. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology, 2017. |
|
Páginas: |
Não paginado. |
|
Idioma: |
Inglês |
|
Palavras-Chave: |
Biomassa de etanol; Etanol lignocelulósico; Hidrólise de celulose; Pichia stipites. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168340/1/Comprovacao-AINFO-Silvia-39th-Symposium-on-Biotechnology-for-Fuels-and-Chemicals.pdf
|
|
Marc: |
LEADER 00723nam a2200193 a 4500
001 2081678
005 2018-01-12
008 2017 bl uuuu u00u1 u #d
100 1 $aSTEFANINI, R.
245 $aM44$ba factorial design analysis of growth kinetics of Pichia stipitis.$h[electronic resource]
260 $aIn: SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS, 39., 2017, São Francisco, CA. [Proceedings ...]. Fairfax: Society for Industrial Microbiology and Biotechnology$c2017
300 $aNão paginado.
653 $aBiomassa de etanol
653 $aEtanol lignocelulósico
653 $aHidrólise de celulose
653 $aPichia stipites
700 1 $aPACHECO, T. F.
700 1 $aGONCALVES, S. B.
700 1 $aMACHADO, F.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
