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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
24/09/2003 |
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Data da última atualização: |
30/03/2023 |
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Autoria: |
FREITAS-ASTUA, J.; ASTUA-MONGE, G.; POLSTON, J. E.; HIEBERT, E. |
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Afiliação: |
Juliana Freitas-Astua, CNPMS; Gustavo Astua-Monge, Instituto Agronômico/Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira; Jane Elisabeth Polston, Universidade Fluminense - UF/Gulf Coast Research & Education Center; Ernest Hiebert, Universidade Fluminense - UF/Plant Pathology. |
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Título: |
A simple and reliable method for the screening of transgenic tobacco plants. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 7, p. 893-896, jul. 2003 |
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Idioma: |
Inglês |
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Notas: |
Notas Científicas.
Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas. |
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Conteúdo: |
Even though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still time-consuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the polymerase chain reaction (PCR) amplification of the transgene using specific primers. |
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Palavras-Chave: |
canamicina; organismo geneticamente modificado; teste. |
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Thesagro: |
Planta Transgênica. |
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Thesaurus Nal: |
genetically modified organisms; kanamycin; testing; transgenic plants. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/24881/1/v38n7a15.pdf
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Marc: |
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100 1 $aFREITAS-ASTUA, J.
245 $aA simple and reliable method for the screening of transgenic tobacco plants.$h[electronic resource]
260 $c2003
500 $aNotas Científicas. Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas.
520 $aEven though much improvement has been made in plant transformation methods, the screening of transgenic plants is often a laborious work. Most approaches for detecting the transgene in transformed plants are still time-consuming, and can be quite expensive. The objective of this study was to search for a simpler method to screen for transgenic plants. The infiltration of kanamycin (100 mg/mL) into tobacco leaves resulted in conspicuous chlorotic spots on the non-transgenic plant leaves, while no spots were seen on the leaves of transformed plants. This reaction occurred regardless of age of the tested plants, and the method has proven to be simple, fast, non-destructive, relatively cheap, and reliable. These results were comparable to those obtained by the polymerase chain reaction (PCR) amplification of the transgene using specific primers.
650 $agenetically modified organisms
650 $akanamycin
650 $atesting
650 $atransgenic plants
650 $aPlanta Transgênica
653 $acanamicina
653 $aorganismo geneticamente modificado
653 $ateste
700 1 $aASTUA-MONGE, G.
700 1 $aPOLSTON, J. E.
700 1 $aHIEBERT, E.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 38, n. 7, p. 893-896, jul. 2003
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Embrapa Unidades Centrais (AI-SEDE) |
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| 1. |  | FREITAS-ASTUA, J.; ASTUA-MONGE, G.; POLSTON, J. E.; HIEBERT, E. A simple and reliable method for the screening of transgenic tobacco plants. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 7, p. 893-896, jul. 2003 Notas Científicas.
Título em português: Método simples e confiável para avaliação de plantas de fumo transgênicas.| Biblioteca(s): Embrapa Unidades Centrais. |
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