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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/03/2011 |
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Data da última atualização: |
15/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLISSENI, J.; SÁ, W. F. de; GUERRA, M. O.; MACHADO, M. A.; SERAPIÃO, R. V.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; PETERS, V. M. |
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Afiliação: |
JULIANA POLISSENI, UFJF; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; MARTHA DE OLIVEIRA GUERRA, UFJF; MARCO ANTONIO MACHADO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFJF; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF. |
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Título: |
Post biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Fertility and Sterility, v. 93. n. 3, p. 783-788, 2010. |
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DOI: |
https://doi.org/10.1016/j.fertnstert.2008.10.023 |
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Idioma: |
Inglês |
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Conteúdo: |
Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres. MenosObjective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell t... Mostrar Tudo |
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Palavras-Chave: |
Embryo quality; Hatching rate; PGD; Sexing. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03686naa a2200265 a 4500
001 1881353
005 2024-02-15
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fertnstert.2008.10.023$2DOI
100 1 $aPOLISSENI, J.
245 $aPost biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis.$h[electronic resource]
260 $c2010
520 $aObjective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.
653 $aEmbryo quality
653 $aHatching rate
653 $aPGD
653 $aSexing
700 1 $aSÁ, W. F. de
700 1 $aGUERRA, M. O.
700 1 $aMACHADO, M. A.
700 1 $aSERAPIÃO, R. V.
700 1 $aCARVALHO, B. C. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aPETERS, V. M.
773 $tFertility and Sterility$gv. 93. n. 3, p. 783-788, 2010.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 37 | |
| 3. |  | DIAS, N. B.; CAMARGO, L. S. A.; SERAPIÃO, R. V.; FOLHADELLA, I. M.; RAMOS, A. A.; POLISSENI, J.; VIANA, J. H. M.; SÁ, W. F.; FERREIRA, A. M. Association of bovine sperm motility and viability with in vitro embryo production. Acta Scientiae Veterinariae, v. 34, s437, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M. Impact of the use of gonadotropins on embryonic development - a rat model. JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | RAMOS, A. de A.; FERREIRA, A. de M.; SÁ, W. F. de; VIANA, J. H. M.; CAMARGO, L. S. de A.; POLISSENI, J.; HENRY, M. Efeito da somatotropina na população folicular, recuperação de oócitos e produção in vitro de embriões em vacas Gir. Revista Brasileira de Zootecnia, Viçosa, MG, v. 36, n. 2, p. 380-386, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | POLISSENI, J.; SÁ, W. F. de; GUERRA, M. O.; MACHADO, M. A.; SERAPIÃO, R. V.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; PETERS, V. M. Post biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis. Fertility and Sterility, v. 93. n. 3, p. 783-788, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | SERAPIÃO, R. V.; BOITÉ, M. C.; CAMARGO, L. S. A.; POLISSENI, J.; VIANA, J. H. M.; FOLHADELLA, I.; SÁ, W. F.; FONSECA, F. A. Effect of replacer of fetal calf serum in the development and gene expression in be embryos in vitro cultured. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 151| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | POLISSENI, J.; CARVALHO, B. C.; PEREIRA, M. M.; SERAPIÃO, R. V.; BATISTA, R. I. T. P.; SILVA, M. R.; BOITÉ, M. C.; CAMARGO, L. S. A.; VIANA, J. H. M.; SÁ, W. F.; IGUMA, L. T. Comparação entre diferentes métodos de separação de espermatozóides: swim up, Isolate ® e Percoll ® na vitalidade e produção in vitro de embriões bovinos. In: CONGRESSO INTERNACIONAL DO LEITE, 6., 2007, Resende. Anais... Juiz de Fora: Embrapa Gado de Leite, 2007. 1 CD.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | SERAPIÃO, R. V.; RAMOS, A. A.; POLISSENI, J.; FOLHADELLA, I. M.; DIAS, N. B.; CAMARGO, L. S. A.; VIANA, J. H. M.; FERREIRA, A. M.; SÁ, W. F.; FONSECA, F. A. Bovine embryos cultured in serum-free medium in different atmospheric conditions. Acta Scientiae Veterinariae, v. 34, s462, 2006. Supl. 1. Edição dos resumos da 20ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Araxá, 2006.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | POLISSENI, J.; GUERRA, M. O.; SERAPIÃO, R. V.; PEREIRA, M. M.; FOLHADELLA, I. M.; SÁ, W. F.; CAMARGO, L. S. A.; VIANA, J. H. M.; IGUMA, L. T.; BOITÉ, M. C.; PETERS, V. M. Biopsy Technique in bovine embryos produced in vitro at early stages of development: evaluation of quality and development capacity. In: REPRODUCTION, FERTILITY AND DEVELOPMENT. 20. 2008, Anais. Colorado. Csiro, Academy of Science, 2008. 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 12. | | POLISSENI, J.; SERAPIÃO, R. V.; CAMPOS JR. P. H. A.; GRAZIA, J. G. V. DE; MILEN, L. C.; CAMARGO, L. S. de A.; GUERRA, M. DE O.; PETERS, V. M. Tecnica de TUNEL em embriões de ratas Wistar: avaliação da qualidade e da capacidade de desenvolvimento dos embriões. Revista Interdisciplinar de Estudos Experimentais, v. 1, n. 3, p. 110-113, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: C - 0 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | POLISSENI, J.; MACHADO, M. A.; AZEVEDO, A. L. S.; DOMINGUES, R.; GUERRA, M. O.; SERAPIÃO, R. V.; PEREIRA, M. M.; SÁ, W. F. DE; CARVALHO, B. C.; CAMARGO, L. S. de A.; VIANA, J. H. M.; PETERS, V. M. Whole genome amplification on blastomers of Post-Biopsy Bovine embryo. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY - REPRODUCTION, FERTILITY AND DEVELOPMENT, 21., 2009, San Diego. Proceedings... San Diego: IETS, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 14. | | POLISSENI, J.; GUERRA, M. de O.; SERAPIÃO, R. V.; PEREIRA, M. M.; FOLHADELLA, I. M.; SÁ, W. F.; CAMARGO, L. S. A.; VIANA, J. H. M.; WOHLRES-VIANA, S.; BOITÉ, M. C.; PETERS, V. M. Viabilidade da técnica de biopsia em embriões bovinos fecundados in vitro em estádios iniciais do desenvolvimento. Acta Scientiae Veterinariae, v. 35, p. s1192, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | SERAPIÃO, R. V.; VIREQUE, A. A.; FERREIRA, E. -M.; PEREIRA, M. M.; POLISSENI, J.; GUIMARÃES, M. F. M.; IGUMA, L. T.; CAMARGO, L. S. A.; VIANA, J. H. M.; SÁ, W. F. de; FONSECA, F. A. Expressão dos transcritos HSP70.1 e BAX em embriões bovinos cultivados em meio suplementado com soro fetal bovino ou Knockout tm SR. Acta Scientiae Veterinariae, v. 35, p. s952, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | BOITÉ, M. C.; NOGUEIRA, L. A. G.; PEREIRA, M. M.; BATISTA, R. I. T. P.; WOHLRES-VIANA, S.; POLISSENI, J.; SERAPIÃO, R. V.; SÁ, W. F.; IGUMA, L. T.; VIANA, J. H. M.; CAMARGO, L. S. A. Comportamento osmótico de embriões bovinos cultivados em meio SOFaa ou CR2aa. Acta Scientiae Veterinariae, v. 35, p. s997, 2007. Supl. 3. Edição dos anais da 21ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Salvador, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | POLISSENI, J.; CARVALHO, B. C.; PEREIRA, M. M.; SERAPIÃO, R. V.; BATISTA, R. I. T. P.; CAMARGO, L. S. A.; VIANA, J. H. M.; SÁ, W. F.; IGUMA, L. T. Comparison of diferent methods of bovine sperm separation: swim up, isolate and percoli on the viability and in vitro embryo production. In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 16., 2008, Budapest. Abstracts... Budapest, 2008. p. 59| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 18. | | POLISSENI, J.; CARVALHO, B. C.; CAMPOS JÚNIOR. P. H. A.; PEREIRA, M. M.; SERAPIÃO, R. V.; BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; VIANA, J. H. M.; SA, W. F. DE; IGUMA, L. T. Comparison of three differents methods of sperm separation: swin up, isolate and percoll. In: WORKSHOP ON MALE REPRODUCTIVE BIOLOGY, 2., 2009, São Paulo. Abstracts... São Paulo: FESBE, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 19. | | CARVALHO, B. C.; SERAPIÃO, R. V.; CAMPOS JR. P. H. A.; POLISSENI, J.; PEREIRA, M. M.; ALVES, B. R. C.; OLIVEIRA, A. P.; VIANA, J. H. M.; CAMARGO, L. S. A.; SÁ, W. F. Criotolerância de embriões bovinos produzidos in vitro cultivados em meio CR2 suplementado com knockout tm SR. Acta Scientiae Veterinariae, v. 36, p. s510, 2008. Supl. 2. s510 Edição dos anais da 22ª Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, 2008.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | SERAPIÃO, R. V.; QUINTAO, C. C. R.; PEREIRA, M. M.; IGUMA, L. T.; CARVALHO, B. C.; POLISSENI, J.; SALES, J. N.; CAMPOS JR. P. H.; OLIVEIRA, A. P.; VIANA, J. H. M.; CAMARGO, L. S. de A. Desenvolvimento de embriões bovinos produzidos pela transferencia nuclear com citoplasma de oocitos maturados in vitro ou in vivo. In: SEMANA DE BIOLOGIA, 32., 2009, Juiz de Fora. Anais... Juiz de Fora: UFJF, 2009.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 37 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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