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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
16/11/2018 |
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Data da última atualização: |
11/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. |
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Afiliação: |
ANNA M. ALESSI, UNIVERSITY OF YORK; SUSANNAH M. BIRD, UNIVERSITY OF YORK; NICOLA C. OATES, UNIVERSITY OF YORK; YI LI, UNIVERSITY OF YORK; ADAM A. DOWLE, UNIVERSITY OF YORK; ETELVINO HENRIQUE NOVOTNY, CNPS; EDUARDO R. DE AZEVEDO, USP; JOSEPH P. BENNETT, UNIVERSITY OF YORK; IGOR POLIKARPOV, USP; J. PETER W. YOUNG, UNIVERSITY OF YORK; SIMON J. MCQUEEN-MASON, UNIVERSITY OF YORK; NEIL C. BRUCE, UNIVERSITY OF YORK. |
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Título: |
Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Biotechnology for Biofuels, v. 11, article 166, 2018. |
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DOI: |
https://doi.org/10.1186/s13068-018-1164-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation. MenosBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacau... Mostrar Tudo |
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Palavras-Chave: |
CAZy; Metasecretome. |
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Thesaurus Nal: |
Lignocellulose. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/186133/1/2018-044.pdf
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Marc: |
LEADER 03644naa a2200301 a 4500
001 2099501
005 2021-11-11
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s13068-018-1164-2$2DOI
100 1 $aALESSI, A. M.
245 $aDefining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.$h[electronic resource]
260 $c2018
520 $aBackground: Lignocellulose is one of the most abundant forms of fixed carbon in the biosphere. Current industrial approaches to the degradation of lignocellulose employ enzyme mixtures, usually from a single fungal species, which are only effective in hydrolyzing polysaccharides following biomass pre-treatments. While the enzymatic mechanisms of lignocellulose degradation have been characterized in detail in individual microbial species, the microbial communities that efficiently breakdown plant materials in nature are species rich and secrete a myriad of enzymes to perform "community-level" metabolism of lignocellulose. Single-species approaches are, therefore, likely to miss important aspects of lignocellulose degradation that will be central to optimizing commercial processes. Results: Here, we investigated the microbial degradation of wheat straw in liquid cultures that had been inoculated with wheat straw compost. Samples taken at selected time points were subjected to multi-omics analysis with the aim of identifying new microbial mechanisms for lignocellulose degradation that could be applied in industrial pretreatment of feedstocks. Phylogenetic composition of the community, based on sequenced bacterial and eukaryotic ribosomal genes, showed a gradual decrease in complexity and diversity over time due to microbial enrichment. Taxonomic affiliation of bacterial species showed dominance of Bacteroidetes and Proteobacteria and high relative abundance of genera Asticcacaulis, Leadbetterella and Truepera. The eukaryotic members of the community were enriched in peritrich ciliates from genus Telotrochidium that thrived in the liquid cultures compared to fungal species that were present in low abundance. A targeted metasecretome approach combined with metatranscriptomics analysis, identified 1127 proteins and showed the presence of numerous carbohydrate-active enzymes extracted from the biomassbound fractions and from the culture supernatant. This revealed a wide array of hydrolytic cellulases, hemicellulases and carbohydrate-binding modules involved in lignocellulose degradation. The expression of these activities correlated to the changes in the biomass composition observed by FTIR and ssNMR measurements. Conclusions: A combination of mass spectrometry-based proteomics coupled with metatranscriptomics has enabled the identification of a large number of lignocellulose degrading enzymes that can now be further explored for the development of improved enzyme cocktails for the treatment of plant-based feedstocks. In addition to the expected carbohydrate-active enzymes, our studies reveal a large number of unknown proteins, some of which may play a crucial role in community-based lignocellulose degradation.
650 $aLignocellulose
653 $aCAZy
653 $aMetasecretome
700 1 $aBIRD, S. M.
700 1 $aOATES, N. C.
700 1 $aLI, Y.
700 1 $aDOWLE, A. A.
700 1 $aNOVOTNY, E. H.
700 1 $aAZEVEDO, E. R. de
700 1 $aBENNETT, J. P.
700 1 $aPOLIKARPOV, I.
700 1 $aYOUNG, J. P. W.
700 1 $aMCQUEEN-MASON, S. J.
700 1 $aBRUCE, N. C.
773 $tBiotechnology for Biofuels$gv. 11, article 166, 2018.
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Registro original: |
Embrapa Solos (CNPS) |
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Biblioteca |
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| Registros recuperados : 16 | |
| 3. |  | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Comparison of fpase and xylanase production between fungi isolated from amazon region and aspergillus niger (F12). In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programas e resumos... [Brasília, DF]: Sociedade Brasileira de Biotecnologia, 2010. p. 327. res. 411.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | PIROTA, R. D. P. B.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Produção de carboximetilcelulase e poligalacturonase sólido pelo fungo Trichoderma polysporum por fermentação em estado sólido, utilizando bagaço de laranja com substrato. In: JORNADA CIENTÍFICA-EMBRAPA SÃO CARLOS, 2009, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação Agropecuária, 2009. p. 11 (Embrapa Pecuária Sudeste. Documentos, 90)| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 5. | | PIROTA, R. D. P. B.; TONELOTTO, M.; DELABONA, P. da S.; POLIKARPOV, I.; FARINAS, C. S. Produção enzimática por fungos isolados da Região Amazônica, visando aplicação na produção de etanol celulósico. In: SEMINÁRIO BRASILEIRO DE TECNOLOGIA ENZIMÁTICA - ENZITEC, 9., 2010, Rio de Janeiro. Versatilidade e eficiência na inovação sustentável: resumos. Rio de Janeiro: UFRJ, 2010. p. 106.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | LEITE, F. L.; OLIVEIRA NETO, M.; BALLESTERO, M. R. M.; POLIKARPOV, I.; MASCARENHAS, Y. P.; HERRMANN, P. S. P.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. Determination of conformation and fractal behavior on poly(0-ethoxyaniline) by atomic force microscopy and small-angle X-Ray scattering. In: MACRO 2006; WORLD POLYMER CONGRESS, Rio de Janeiro, Brazil; INTERNATIONAL SYMPOSIUM ON MACROMOLECULES, 41ST., 2006, Rio de Janeiro. Proceedings... [S. l.]: ABPol, 2006. 1 CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
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| 7. | | LEITE, F. L.; ALVES, W. F.; OLIVEIRA NETO, M.; POLIKARPOV, I.; HERRMANN, P. S. P.; MATTOSO, L. H. C.; OLIVEIRA, JUNIOR, O. N. Doping in poly (o-ethoxyaniline) nanostructured films studied with atomic force spectroscopy (AFS). Micron, New York, v. 39, p. 1119-1125, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Instrumentação. |
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| 8. | | TONELOTTO, M. PIROTA, R. D. P. B. DELABONA, P. S. BARROS, G. O. F.; GOLUBEV, A. M. POLIKARPOV, I.; FARINAS, C. S. Isolation and characterization of a ß-galactosidase from a now Amazon forest strain of Aspergillus niger as a potential accessory enzyme for biomass conversion. Biocatalysis and Biotransformation, [S. l.], v. 32, n. 1, p. 13-22, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 9. | | LIMA, M. A.; GOMEZ, L. D.; STEELI-KING, C. G.; SIMISTER, R.; BERNARDINELLI, O. D.; CARVALHO, M. A.; REZENDE, C. A.; LABATE, C. A.; AZEVEDO, E. R.; McQUEEN-MASON, S.; POLIKARPOV, I.; CARVALHO, M. A. Evaluating the composition and processing potential of novel sources of Brazilian biomass for sustainable biorenewables production. Biotechnology for Biofuels, v. 7, n. 10, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Cerrados. |
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| 10. | | ALESSI, A. M.; BIRD, S. M.; OATES, N. C.; LI, Y.; DOWLE, A. A.; NOVOTNY, E. H.; AZEVEDO, E. R. de; BENNETT, J. P.; POLIKARPOV, I.; YOUNG, J. P. W.; MCQUEEN-MASON, S. J.; BRUCE, N. C. Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies. Biotechnology for Biofuels, v. 11, article 166, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 11. | | LEITE, F. de L.; SIMÕES, M. L.; OLIVEIRA NETO, M. de; SAVEDRA, R. M. L.; HERRMANN JUNIOR, P. S. de P.; POLIKARPOV, I.; MARTIN NETO, L.; TRSIC, M.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. de. A Nanocarrier charge model for the transport in poly(o-alkoxyanilines) [PTu19]. In: INTERNATIONAL CONFERENCE ON SCIENCE AND TECHNOLOGY OF SYNTHETIC METALS, ICSM, 2008, Porto de Galinhas. Book of Abstracts... [S. l.: s. n.], 2008. p. 61.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | HERRMANN, P. S. de P.; MASCARENHAS, Y. P.; POLIKARPOV, I.; BALLESTERO, M. R. M.; OLIVEIRA NETO, M. de; LEITE, F. de L.; MATTOSO, L. H. C.; OLIVEIRA, O. N. de. Nanoscale conformational ordering on poly(alkoxyanilines): fractal behavior and thin films formation mechanism. In: ENCONTRO DA SBPMAT, 5.; BRAZILIAN MRS MEETING, 5TH., 2006, Florianópolis. Final program... Florianópolis: [s. n.], 2006. p. 111. 1CD-ROM.| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | LEITE, F. L.; OLIVEIRA NETO, M. de; PATERNO, L. G.; BALLESTERO, M. R. M.; POLIKARPOV, I.; MASCARENHAS, Y. P.; HERRMANN, P. S. P.; MATTOSO, L. H. C.; OLIVEIRA JUNIOR, O. N. Nanoscale conformational ordering in polyanilines investigated by SAXS and AFM Journal of Colloid and Interface Science, New York, v. 316, p. 376-387, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Instrumentação. |
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| 14. | | ESPÍRITO SANTO, M. C. do; CARDOSO, E. B.; GUIMARAES, F. E. G.; AZEVEDO, E. R. de; CUNHA, G. P. da; NOVOTNY, E. H.; PELLEGRINI, V. de O. A.; CHANDEL, A. K.; SILVEIRA, M. H. L.; POLIKARPOV, I. Multifaceted characterization of sugarcane bagasse under different steam explosion severity conditions leading to distinct enzymatic hydrolysis yields. Industrial Crops & Products, v. 139, 111542, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 15. | | COLUSSI, F.; SERPA, V.; DELABONA, P. da S.; MANZINE, L. R.; VOLTATÓDIO, M. L.; ALVES, R.; MELLO, B. L.; PEREIRA JUNIOR, N.; FARINAS, C. S.; GOLUBEV, A. M.; SANTOS, M. A. M.; POLIKARPOV, I. Purification, and biochemical and biophysical characterization of cellobiohydrolase I from trichoderma harzianum IOC 3844. Journal of microbiology and biotechnology, Kangnam-Gu, v. 21, n. 8, p. 808-817, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Instrumentação. |
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| 16. | | ESPIRITO SANTO, M. C. do; THEMA, F. T.; PELLEGRINI, V. de O. A.; KANE, A. O.; GUIMARAES, F. E. G.; FILGUEIRAS, J. G.; NOVOTNY, E. H.; AZEVEDO, E. R. de; POLIKARPOV, I. When the order matters: impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse. Industrial Crops and Products, v. 180, 114708, Jun. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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