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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
04/02/2014 |
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Data da última atualização: |
19/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, F. A. C.; PIROVANI, C. P.; MENEZES, S.; PUNGARTNIK, C.; SANTIAGO, A. S.; COSTA, M. G. C.; MICHELI, F.; GESTEIRA, A. da S. |
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Afiliação: |
F. A. C. SILVA, UESC; C. P. PIROVANI, UESC; S. MENEZES, UESC; C. PUNGARTNIK, UESC; A. S. SANTIAGO, UESC; M. G. C. COSTA, UESC; F. MICHELI, Agricultural Research for Development; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Proteomic response of Moniliophthora perniciosa exposed to pathogenesis-related protein-10 from Theobroma cacao. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.12, n. 4, p. 4855-4868, 2013. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
TcPR-10, a member of the pathogenesis-related protein 10 family, was identified in EST library of interactions between Theobroma cacao and Moniliophthora perniciosa. TcPR-10 has been shown to have antifungal and ribonuclease activities in vitro. This study aimed to identify proteins that are differentially expressed in M. perniciosa in response to TcPR-10 through a proteomic analysis. The fungal hyphae were subjected to one of four treatments: control treatment or 30-, 60- or 120-min treatment with the TcPR-10 protein. Two-dimensional maps revealed 191 differentially expressed proteins, 55 of which were identified by mass spectrometry. The proteins identified in all treatments were divided into the following classes: cell metabolism, stress response, zinc binding, phosphorylation mechanism, transport, autophagy, DNA repair, and oxidoreductases. The predominant class was stress-response proteins (29%), such as heat shock proteins; these proteins exhibited the highest expression levels relative to the control treatment and are known to trigger defense mechanisms against cytotoxic drugs as well as TcPR-10. Oxidoreductases (25%) were overexpressed in the control and in 30-min treatments but exhibited reduced expression at 120 min. These proteins are involved in the repair of damage caused by oxidative stress due to the contact with TcPR- 10. Consistent with the antifungal activity of TcPR-10, several proteins identified were related to detoxification, autophagy or were involved in mechanisms for maintaining fungal homeostasis, such as ergosterol biosynthesis. These results show that the sensitivity of the fungus to TcPR-10 involves several biochemical routes, clarifying the possible modes of action of this antifungal protein. MenosTcPR-10, a member of the pathogenesis-related protein 10 family, was identified in EST library of interactions between Theobroma cacao and Moniliophthora perniciosa. TcPR-10 has been shown to have antifungal and ribonuclease activities in vitro. This study aimed to identify proteins that are differentially expressed in M. perniciosa in response to TcPR-10 through a proteomic analysis. The fungal hyphae were subjected to one of four treatments: control treatment or 30-, 60- or 120-min treatment with the TcPR-10 protein. Two-dimensional maps revealed 191 differentially expressed proteins, 55 of which were identified by mass spectrometry. The proteins identified in all treatments were divided into the following classes: cell metabolism, stress response, zinc binding, phosphorylation mechanism, transport, autophagy, DNA repair, and oxidoreductases. The predominant class was stress-response proteins (29%), such as heat shock proteins; these proteins exhibited the highest expression levels relative to the control treatment and are known to trigger defense mechanisms against cytotoxic drugs as well as TcPR-10. Oxidoreductases (25%) were overexpressed in the control and in 30-min treatments but exhibited reduced expression at 120 min. These proteins are involved in the repair of damage caused by oxidative stress due to the contact with TcPR- 10. Consistent with the antifungal activity of TcPR-10, several proteins identified were related to detoxification, autophagy or were involved ... Mostrar Tudo |
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Palavras-Chave: |
Differentially expressed proteins; Stress-response proteins. |
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Thesagro: |
Cacau; Doença de planta; Theobroma Cacao. |
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Thesaurus Nal: |
Oxidoreductases; Pathogenesis-related proteins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02677naa a2200301 a 4500
001 1978540
005 2023-05-19
008 2013 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aSILVA, F. A. C.
245 $aProteomic response of Moniliophthora perniciosa exposed to pathogenesis-related protein-10 from Theobroma cacao.$h[electronic resource]
260 $c2013
520 $aTcPR-10, a member of the pathogenesis-related protein 10 family, was identified in EST library of interactions between Theobroma cacao and Moniliophthora perniciosa. TcPR-10 has been shown to have antifungal and ribonuclease activities in vitro. This study aimed to identify proteins that are differentially expressed in M. perniciosa in response to TcPR-10 through a proteomic analysis. The fungal hyphae were subjected to one of four treatments: control treatment or 30-, 60- or 120-min treatment with the TcPR-10 protein. Two-dimensional maps revealed 191 differentially expressed proteins, 55 of which were identified by mass spectrometry. The proteins identified in all treatments were divided into the following classes: cell metabolism, stress response, zinc binding, phosphorylation mechanism, transport, autophagy, DNA repair, and oxidoreductases. The predominant class was stress-response proteins (29%), such as heat shock proteins; these proteins exhibited the highest expression levels relative to the control treatment and are known to trigger defense mechanisms against cytotoxic drugs as well as TcPR-10. Oxidoreductases (25%) were overexpressed in the control and in 30-min treatments but exhibited reduced expression at 120 min. These proteins are involved in the repair of damage caused by oxidative stress due to the contact with TcPR- 10. Consistent with the antifungal activity of TcPR-10, several proteins identified were related to detoxification, autophagy or were involved in mechanisms for maintaining fungal homeostasis, such as ergosterol biosynthesis. These results show that the sensitivity of the fungus to TcPR-10 involves several biochemical routes, clarifying the possible modes of action of this antifungal protein.
650 $aOxidoreductases
650 $aPathogenesis-related proteins
650 $aCacau
650 $aDoença de planta
650 $aTheobroma Cacao
653 $aDifferentially expressed proteins
653 $aStress-response proteins
700 1 $aPIROVANI, C. P.
700 1 $aMENEZES, S.
700 1 $aPUNGARTNIK, C.
700 1 $aSANTIAGO, A. S.
700 1 $aCOSTA, M. G. C.
700 1 $aMICHELI, F.
700 1 $aGESTEIRA, A. da S.
773 $tGenetics and Molecular Research$gv.12, n. 4, p. 4855-4868, 2013.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Florestas; Embrapa Gado de Leite. |
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Data corrente: |
25/10/2023 |
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Data da última atualização: |
07/11/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MADARI, B. E.; MATOS, P. S.; OLIVEIRA, J. M.; CARVALHO, M. T. de M.; SILVEIRA, A. L. R.; MACHADO, P. L. O. de A.; ARAUJO, W. A.; SIQUEIRA, M. M. B.; FERRARESI, T. M.; FREITAS, F. M. C.; RIZZO, P. V.; SILVA, R. R.; PACHECO, A. |
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Afiliação: |
BEATA EMOKE MADARI, CNPAF; PRISCILA SILVA MATOS, bolsista CNPAF; JANAINA MOURA OLIVEIRA, FACULDADE METROPOLITANA DE ANÁPOLIS; MARCIA THAIS DE MELO CARVALHO, CNPAF; ANDRE LUIZ RODRIGUES SILVEIRA; PEDRO LUIZ OLIVEIRA DE A MACHADO, CNPAF; WILKER ALVES DE ARAUJO, bolsista CNPAF; MATHEUS MENTONE DE BRITTO SIQUEIRA, bolsista CNPAF; TATIANA MARIS FERRARESI, CNPAF; FERNANDA MARA CUNHA FREITAS, CNPGL; PRICILA VETRANO RIZZO, CNPGL; RYAN RODRIGUES DA SILVA, bolsista CNPAF; ABILIO RODRIGUES PACHECO, CNPF. |
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Título: |
Carbono orgánico del suelo en cultivos-ganadería-silvicultura en un estudio de caso de la región centro-oeste de Brasil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONGRESO INTERNACIONAL DE SISTEMAS SILVOPASTORILES, 12.; CONGRESO DE LA RED GLOBAL DE SISTEMAS SILVOPASTORILES, 2.; IV SEMINARIO NACIONAL DE SISTEMAS SILVOPASTORILES, 4., 2023, Montevideo; CONGRESO NACIONAL SISTEMAS SILVOPASTORILES, 5., 2023, Buenos Aires. Sistemas silvopastoriles: hacia una diversificación sostenible. Cali: CIPAV, 2023. |
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Páginas: |
p. 547-560. |
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ISBN: |
978-628-95190-5-1 |
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Idioma: |
Espanhol |
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Conteúdo: |
ABSTRACT- Crop-livestock-forestry (CLF) has been recommended as a strategy for soil carbon sequestration, reducing greenhouse gas (GHG) emissions of livestock as well as for sustainable soil management for improving soil organic carbon (SOC) and soil health. In this study we quantified SOC accruals in a very clayey Rhodic Ferralsol during the evolution of a 11-year-old crop-livestock-forestry system in the municipality of Cachoeira Dourada, Central West region of Brazil. CLF was implemented in 2009 in a conventional Pasture area, and soil samples were collected in 2012 and 2020 under two land uses, CLF, and the conventional Pasture as time-zero reference. The soil in CLF was evaluated within the tree lines (CLF-WL) and in the alley pasture between the tree lines (CLF-BL) having palisade grass. SOC dynamics were different in the upper 30 cm and in the underlying 70 cm layer. In the 0.0-0.3 m layer, after an initial decline in SOC stocks due to soil preparation for the implementation of CLF, SOC accumulation rates were positive in CLF (0.46-0.28 Mg ha-1 yr-1 ), but negative in the reference Pasture (-0.26). At 0.3-1.0 m SOC stocks were higher (both in 2012 and 2020) in the CLF-WL (86.70 and 83.74) than in the Pasture (71.81 and 74.96), however, CLF SOC was declining. Total soil N under CLF, compared to the reference Pasture, was lower and declining. These findings confirm that in general CLF is a valid strategy to increase SOC stocks compared to conventional Pasture, but adequate soil fertility management is necessary to potentialize SOC.
RESUMEN- Se ha recomendado a la agricultura-ganadería-silvicultura (AGS) como estrategia para el secuestro de carbono del suelo, la reducción de las emisiones de gases de efecto invernadero (GEI) del ganado, así como para el manejo sostenible del suelo para mejorar el carbono orgánico del suelo (COS) y la salud del suelo. En este estudio cuantificamos la acumulación de COS en un Ferralsol Ródico muy arcilloso durante la evolución de un sistema agrícolaganadero-forestal de 11 años de antigüedad en el municipio de Cachoeira Dourada, región Centro Oeste de Brasil. AGS se implementó en 2009 en un área de pastos convencionales, y se recolectaron muestras de suelo en 2012 y 2020 bajo dos usos de tierra, AGS y pastos convencionales como referencia de tiempo cero. El suelo en AGS se evaluó dentro de las líneas de árboles (AGS-DL) y en el pasto en callejón entre las líneas de árboles (AGS-EL) que tenía pasto en empalizada. La dinámica del COS fue diferente en los 30 cm superiores y en la capa subyacente de 70 cm. En la capa de 0,0-0,3 m, después de una disminución inicial en las existencias de COS debido a la preparación del suelo para la implementación de AGS, las tasas de acumulación de COS fueron positivas en AGS (0,46-0,28 Mg ha-1 año-1), pero negativas en la capa de referencia. Pasto (-0,26). A 0,3-1,0 m, las existencias de COS fueron mayores (tanto en 2012 como 2020) en AGS-DL (86,70 y 83,74) que en Pastizales (71,81 y 74,96), sin embargo, AGS SOC estaba disminuyendo. El N total del suelo bajo AGS, en comparación con el pasto de referencia, fue menor y disminuyó. Estos hallazgos confirman que, en general, la AGS es una estrategia válida para aumentar las reservas de COS en comparación con los pastos convencionales, pero es necesario un manejo adecuado de la fertilidad del suelo para potencializar el COS. MenosABSTRACT- Crop-livestock-forestry (CLF) has been recommended as a strategy for soil carbon sequestration, reducing greenhouse gas (GHG) emissions of livestock as well as for sustainable soil management for improving soil organic carbon (SOC) and soil health. In this study we quantified SOC accruals in a very clayey Rhodic Ferralsol during the evolution of a 11-year-old crop-livestock-forestry system in the municipality of Cachoeira Dourada, Central West region of Brazil. CLF was implemented in 2009 in a conventional Pasture area, and soil samples were collected in 2012 and 2020 under two land uses, CLF, and the conventional Pasture as time-zero reference. The soil in CLF was evaluated within the tree lines (CLF-WL) and in the alley pasture between the tree lines (CLF-BL) having palisade grass. SOC dynamics were different in the upper 30 cm and in the underlying 70 cm layer. In the 0.0-0.3 m layer, after an initial decline in SOC stocks due to soil preparation for the implementation of CLF, SOC accumulation rates were positive in CLF (0.46-0.28 Mg ha-1 yr-1 ), but negative in the reference Pasture (-0.26). At 0.3-1.0 m SOC stocks were higher (both in 2012 and 2020) in the CLF-WL (86.70 and 83.74) than in the Pasture (71.81 and 74.96), however, CLF SOC was declining. Total soil N under CLF, compared to the reference Pasture, was lower and declining. These findings confirm that in general CLF is a valid strategy to increase SOC stocks compared to conventional Pasture, but adeq... Mostrar Tudo |
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Palavras-Chave: |
Carbono orgânico; Lavoura-pecuária-silvicultura; Sequestro de carbono. |
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Thesagro: |
Carbono; Nitrogênio; Solo. |
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Thesaurus NAL: |
Carbon sequestration; Savannas; Soil; Soil organic nitrogen. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/257196/1/CongressoInternacional-Sistemas-Silvopastoriles-2023.pdf
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Marc: |
LEADER 04886nam a2200397 a 4500
001 2157536
005 2023-11-07
008 2023 bl uuuu u00u1 u #d
020 $a978-628-95190-5-1
100 1 $aMADARI, B. E.
245 $aCarbono orgánico del suelo en cultivos-ganadería-silvicultura en un estudio de caso de la región centro-oeste de Brasil.$h[electronic resource]
260 $aIn: CONGRESO INTERNACIONAL DE SISTEMAS SILVOPASTORILES, 12.; CONGRESO DE LA RED GLOBAL DE SISTEMAS SILVOPASTORILES, 2.; IV SEMINARIO NACIONAL DE SISTEMAS SILVOPASTORILES, 4., 2023, Montevideo; CONGRESO NACIONAL SISTEMAS SILVOPASTORILES, 5., 2023, Buenos Aires. Sistemas silvopastoriles: hacia una diversificación sostenible. Cali: CIPAV$c2023
300 $ap. 547-560.
520 $aABSTRACT- Crop-livestock-forestry (CLF) has been recommended as a strategy for soil carbon sequestration, reducing greenhouse gas (GHG) emissions of livestock as well as for sustainable soil management for improving soil organic carbon (SOC) and soil health. In this study we quantified SOC accruals in a very clayey Rhodic Ferralsol during the evolution of a 11-year-old crop-livestock-forestry system in the municipality of Cachoeira Dourada, Central West region of Brazil. CLF was implemented in 2009 in a conventional Pasture area, and soil samples were collected in 2012 and 2020 under two land uses, CLF, and the conventional Pasture as time-zero reference. The soil in CLF was evaluated within the tree lines (CLF-WL) and in the alley pasture between the tree lines (CLF-BL) having palisade grass. SOC dynamics were different in the upper 30 cm and in the underlying 70 cm layer. In the 0.0-0.3 m layer, after an initial decline in SOC stocks due to soil preparation for the implementation of CLF, SOC accumulation rates were positive in CLF (0.46-0.28 Mg ha-1 yr-1 ), but negative in the reference Pasture (-0.26). At 0.3-1.0 m SOC stocks were higher (both in 2012 and 2020) in the CLF-WL (86.70 and 83.74) than in the Pasture (71.81 and 74.96), however, CLF SOC was declining. Total soil N under CLF, compared to the reference Pasture, was lower and declining. These findings confirm that in general CLF is a valid strategy to increase SOC stocks compared to conventional Pasture, but adequate soil fertility management is necessary to potentialize SOC. RESUMEN- Se ha recomendado a la agricultura-ganadería-silvicultura (AGS) como estrategia para el secuestro de carbono del suelo, la reducción de las emisiones de gases de efecto invernadero (GEI) del ganado, así como para el manejo sostenible del suelo para mejorar el carbono orgánico del suelo (COS) y la salud del suelo. En este estudio cuantificamos la acumulación de COS en un Ferralsol Ródico muy arcilloso durante la evolución de un sistema agrícolaganadero-forestal de 11 años de antigüedad en el municipio de Cachoeira Dourada, región Centro Oeste de Brasil. AGS se implementó en 2009 en un área de pastos convencionales, y se recolectaron muestras de suelo en 2012 y 2020 bajo dos usos de tierra, AGS y pastos convencionales como referencia de tiempo cero. El suelo en AGS se evaluó dentro de las líneas de árboles (AGS-DL) y en el pasto en callejón entre las líneas de árboles (AGS-EL) que tenía pasto en empalizada. La dinámica del COS fue diferente en los 30 cm superiores y en la capa subyacente de 70 cm. En la capa de 0,0-0,3 m, después de una disminución inicial en las existencias de COS debido a la preparación del suelo para la implementación de AGS, las tasas de acumulación de COS fueron positivas en AGS (0,46-0,28 Mg ha-1 año-1), pero negativas en la capa de referencia. Pasto (-0,26). A 0,3-1,0 m, las existencias de COS fueron mayores (tanto en 2012 como 2020) en AGS-DL (86,70 y 83,74) que en Pastizales (71,81 y 74,96), sin embargo, AGS SOC estaba disminuyendo. El N total del suelo bajo AGS, en comparación con el pasto de referencia, fue menor y disminuyó. Estos hallazgos confirman que, en general, la AGS es una estrategia válida para aumentar las reservas de COS en comparación con los pastos convencionales, pero es necesario un manejo adecuado de la fertilidad del suelo para potencializar el COS.
650 $aCarbon sequestration
650 $aSavannas
650 $aSoil
650 $aSoil organic nitrogen
650 $aCarbono
650 $aNitrogênio
650 $aSolo
653 $aCarbono orgânico
653 $aLavoura-pecuária-silvicultura
653 $aSequestro de carbono
700 1 $aMATOS, P. S.
700 1 $aOLIVEIRA, J. M.
700 1 $aCARVALHO, M. T. de M.
700 1 $aSILVEIRA, A. L. R.
700 1 $aMACHADO, P. L. O. de A.
700 1 $aARAUJO, W. A.
700 1 $aSIQUEIRA, M. M. B.
700 1 $aFERRARESI, T. M.
700 1 $aFREITAS, F. M. C.
700 1 $aRIZZO, P. V.
700 1 $aSILVA, R. R.
700 1 $aPACHECO, A.
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