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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DÓRIA, M. S.; SOUSA, A. O. de; BARBOSA, C. de J.; COSTA, M. G. C.; GESTEIRA, A. da S.; SOUZA, R. M.; FREITAS, A. C. O.; PIROVANI, C. P. |
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Afiliação: |
MILENA SANTOS DÓRIA, UESC; AURIZÂNGELA OLIVEIRA DE SOUSA, UESC; CRISTIANE DE JESUS BARBOSA, CNPMF; MÁRCIO GILBERTO CARDOSO COSTA, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; REGINA MARTINS SOUZA; ANA CAMILA OLIVEIRA FREITAS, UESC; CARLOS PRIMINHO PIROVANI, UESC. |
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Título: |
Citrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin". |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, July, 2015. |
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ISSN: |
1932-6203 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus. MenosCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTq... Mostrar Tudo |
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Palavras-Chave: |
Plant disease. |
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Thesagro: |
Doença de planta; Fruta cítrica; Vírus. |
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Thesaurus Nal: |
Citrus. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02591naa a2200277 a 4500
001 2027385
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aDÓRIA, M. S.
245 $aCitrus tristeza virus (CTV) causing proteomic and enzymatic changes in sweet orange variety "Westin".$h[electronic resource]
260 $c2015
520 $aCitrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as in the activity and gene expression of antioxidant enzymes. The CTV-infected sweet orange displayed mild symptoms, which were characterized by the presence of sparse stem pitting throughout their stems. The presence of virus was confirmed by RT-PCR. Proteomic analysis by 2DE-PAGE-MS / MS revealed the identity of 40 proteins differentially expressed between CTV- infected and -non-infected samples. Of these, 33 were up-regulated and 7 were down-regulated in CTV-infected samples. Among the proteins identified stands out a specific from the virus, the coat protein. Other proteins identified are involved with oxidative stress and for this their enzymatic activity was measured. The activity of superoxide dismutase (SOD) was higher in CTV-infected samples, as catalase (CAT) showed higher activity in uninfected samples. The activity of guaiacol peroxidase (GPX) did not vary significantly between samples. However, ascorbate peroxidase (APX) was more active in the infected samples. The relative expression of the genes encoding CAT, SOD, APX and GPX was analyzed by quantitative real time PCR (RTqPCR). The CTV-infected samples showed greater accumulation of transcripts, except for the CAT gene. This gene showed higher expression in the uninfected samples. Taken together, it can be concluded that the CTV affects the protein profile and activity and gene expression of antioxidant enzymes in plants infected by this virus.
650 $aCitrus
650 $aDoença de planta
650 $aFruta cítrica
650 $aVírus
653 $aPlant disease
700 1 $aSOUSA, A. O. de
700 1 $aBARBOSA, C. de J.
700 1 $aCOSTA, M. G. C.
700 1 $aGESTEIRA, A. da S.
700 1 $aSOUZA, R. M.
700 1 $aFREITAS, A. C. O.
700 1 $aPIROVANI, C. P.
773 $tPlos One, July, 2015.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com cnph.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
12/11/2009 |
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Data da última atualização: |
24/02/2010 |
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Tipo da produção científica: |
Artigo de Divulgação na Mídia |
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Autoria: |
NASCIMENTO, W. M. |
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Afiliação: |
Warley Marcos Nascimento, Embrapa Hortaliças. |
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Título: |
Comece pelas mudas. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Revista Campo & Negócios HF, Uberlândia, ano 5, n. 51, p. 26-28, ago. 2009. |
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Idioma: |
Português |
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Palavras-Chave: |
Ambiente controlado; Bandeja; Transplantio. |
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Thesagro: |
Hortaliça; Muda; Qualidade; Semente; Tecnologia; Temperatura; Tratamento. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00576naa a2200229 a 4500
001 1782816
005 2010-02-24
008 2009 bl uuuu u00u1 u #d
100 1 $aNASCIMENTO, W. M.
245 $aComece pelas mudas.
260 $c2009
650 $aHortaliça
650 $aMuda
650 $aQualidade
650 $aSemente
650 $aTecnologia
650 $aTemperatura
650 $aTratamento
653 $aAmbiente controlado
653 $aBandeja
653 $aTransplantio
773 $tRevista Campo & Negócios HF, Uberlândia, ano 5$gn. 51, p. 26-28, ago. 2009.
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