Data/hora: 27/05/2024 17:10:11

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	08/09/2014	
Data da última atualização: 	25/05/2023	
Autoria: 	LABATE, M. T. V.; Ko, KENTON; Ko, Z. W.; PINTO, L. S. R. C.; REAL, M. J. U. D.; ROMANO, M. R.; BARJA, P. R.; GRANELL, A.; FRISO, G.; WIJK, K. J. V.; BRUGNOLI, E.; LABATE, C. A.	
Afiliação: 	MÔNICA T. V. LABATE, ESALQ; KENTON KO, Queen’s University; ZDENKA W. Ko, Queen’s University; LUCIANA S. R. COSTA PINTO, ESALQ; MARIA J. U. D. REAL, ESALQ; MARCELO RIBEIRO ROMANO, CNPMF; PAULO ROXO BARJA, ESALQ; ANTONIO GRANELL, Universidad Politecnica de Valencia; GIULIA FRISO, Cornell University; KLAAS J. VAN WIJK, Cornell University; ENRICO BRUGNOLI, Instituto di Biologia Agroambientale; CARLOS A. LABATE, ESALQ.	
Título: 	Constitutive expression of pea Lhcb1-2 in tobacco affects plant development, morphology and photosynthetic capacity.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Plant Molecular Biology, v. 55, p. 701-714, 2014.	
ISSN: 	0167-4412	
Idioma: 	Inglês	
Conteúdo: 	Lhcb1-2 from pea was constitutively expressed in transgenic tobacco plants and assessed for functional impact. The successful assembly of the encoded proteins into LHCII trimers was confirmed by electrospray tandem mass spectrometry. Constitutive production of LHCb1-2 led to increased number of thylakoid membranes per chloroplast, increased grana stacking, higher chloroplast numbers per palisade cell and increased photosynthetic capacity at low irradiance, both on a chlorophyll and leaf area basis. The transgenic plants also displayed increased cell volume, larger leaves, higher leaf number per plant at flowering, increased biomass and increased seed weight, when grown under low irradiance levels. Under high irradiance, both transgenic and wild type plants displayed similar photosynthetic rates when tested at 25 C; however, the non-photochemical quenching (NPQ) and qE values increased in the transgenic plants. The exposure of transgenic plants to a photoinhibitory treatment (4 C for 4 h, under continuous illumination) resulted in more detrimental impairment of photosynthesis, since recovery was slower than the nontransgenic plants. These data indicate that constitutive expression of additional Lhcb1-2 transgenes led to a series of changes at all levels of the plant (cellular, leaf and whole organism), and a delay in flowering and senescence. The additional production of the pea protein appears to be accommodated by increasing cellular structures such as the number of thylakoids per chloroplast, organelle volume, organelles per cell, and leaf expansion. The presence of the trimeric pea protein in the tobacco LHCII, however, caused a possible change in the organization of the associated super-complex, that in turn limited photosynthesis at low temperature	
Palavras-Chave: 	Tabaco.	
Thesagro: 	Clorofila; Ervilha; Fumo.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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