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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/05/1996 |
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Data da última atualização: |
31/05/1996 |
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Autoria: |
PINTO, A. C. de Q. |
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Título: |
In vivo and in vitro growth of immature embryos of peach (Prunus persica (L.) Batsch). |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
[College Station] : Texas A & M University, 1992. |
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Páginas: |
95p. |
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Idioma: |
Inglês |
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Notas: |
PhD. Thesis. |
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Conteúdo: |
Media and novel in vitro manipulation and techniques may improve growth of immature (< 10 mm ) peach embryos. The objectives of thisw study were to evaluate a) in vivo growth of fruts and seed parts and nutriente contents of the developing peach embryos, b) in ovelo growth and quality of immature embryos ( 8.7 mm) as influenced by media (MSM or SHM), sucrose levels (6% or 10%), ovule support systems (vermiculite, VS; saolid Gelrite, GS; filter paper, FPS), and c) in ovulo growth and nutrient contents of small embryos (<5 mm ) as influenced by cultural techniques (intact and perforated oveles, or embryo nurse), ovule support system (VS or semi- solid agar, SSA), media (SHM or Monnier), Hormones (0, 0.25, 050 mg.l-1 LAA plus 0.01 mg.l-1 kinetin), and L-glutamine (none or 400 mg. l-1). In vivo growth trends among cultivars varied in response to the temperature and fruiting set differences between the years. Higher mean temperatura and low fruit set promoted faster embryo growth. Fruit fresh weight, ovele length, and ovule fresh wieght showed low and inconsistent correlations with embryo length and embryo dry weight; they could not be used as a nondestructive method for embryo selection in the field. In vivo embryos had higher macronutrient concentrations than in vitro embryos. However, in vitro embryos showed higher dry matter (24%) as compared to in vivo embryos (12%) which may express a batter quality embryos. Embryos from ovule perforation treatments showed lower nutrient concentrations than those from intact ovules, although their growth was higher in length and dry weight. The ovule support system influenced embruo (8.7 mm) growth more then media or sucrose. The VS system promoted two to four-fold higher embryo dry weight FPS or GS, basides being easier to set up and handle than FPS or GS suystem. The effect of sucrose varied with the medium used; maxsimum embryo growth occurred winth 10% sucrose (w/v) on either SHM or MSM medimum, although SHM at 6% sucrose promoted equal growth as SHM with 10% sucrose. The SHM medium was as effective as Monnier medium on growth of small (<5 mm) embryos, but their effectiveness depended on the technique and ovule support system used, Neither SHM nor Monnier medium was effective on Goldprince embryo growteh (length) when the embryo nurse (EN) technique was used. THE ovule perfotion (OP) and VS system cvonsistently promoted 60 to 100% more embryo growth the conventional intact ovules and the EN techniques. Monnier medium, 12%sucrose, with OP/VS conbination promoted four to two-fold higher embryo dry weight of P51-2 and Goldcrest peaches than OP plus semi-solid agar support (SSA). The monnier medium with 12% sucrose, and OP/VS increased Sprigcrestembryos (2.3 mm) up to 9.6 mm in 30 days (1mm of growth per each 5 days in culture). The effect amino acid L-glutamine varied with genotypes, embryo age, and hormones. A negative effect on growth of Golgprince embryoa (4 mm) occurred whed L-glutamine was used witeh SHM but for Ear ligrandes 1.5 mm embryos a growth effect was found. Without hormones, the SHM medium + L glutamine promoted greater ovule and embryo growth than SHM plus homones.Finally, for an avarege of 35% seedling recovery rate the following sbase line growth criterion is needed: FDP > 75 days, B) ovule dry weight 38 to 96 mg (mean of 51 mg), c) PDW of ovule between 10% and 28% (mean 17%), d) embryo length > 10 mm, and e) PF1 >60%. MenosMedia and novel in vitro manipulation and techniques may improve growth of immature (< 10 mm ) peach embryos. The objectives of thisw study were to evaluate a) in vivo growth of fruts and seed parts and nutriente contents of the developing peach embryos, b) in ovelo growth and quality of immature embryos ( 8.7 mm) as influenced by media (MSM or SHM), sucrose levels (6% or 10%), ovule support systems (vermiculite, VS; saolid Gelrite, GS; filter paper, FPS), and c) in ovulo growth and nutrient contents of small embryos (<5 mm ) as influenced by cultural techniques (intact and perforated oveles, or embryo nurse), ovule support system (VS or semi- solid agar, SSA), media (SHM or Monnier), Hormones (0, 0.25, 050 mg.l-1 LAA plus 0.01 mg.l-1 kinetin), and L-glutamine (none or 400 mg. l-1). In vivo growth trends among cultivars varied in response to the temperature and fruiting set differences between the years. Higher mean temperatura and low fruit set promoted faster embryo growth. Fruit fresh weight, ovele length, and ovule fresh wieght showed low and inconsistent correlations with embryo length and embryo dry weight; they could not be used as a nondestructive method for embryo selection in the field. In vivo embryos had higher macronutrient concentrations than in vitro embryos. However, in vitro embryos showed higher dry matter (24%) as compared to in vivo embryos (12%) which may express a batter quality embryos. Embryos from ovule perforation treatments showed lower nutrien... Mostrar Tudo |
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Palavras-Chave: |
Growth; Peach; Plant embryo; Seed. |
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Thesagro: |
Crescimento; Embrião; Germinação; Pêssego; Prunus Persica; Semente. |
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Thesaurus Nal: |
germination. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04132nam a2200265 a 4500
001 1556347
005 1996-05-31
008 1992 bl uuuu m 00u1 u #d
100 1 $aPINTO, A. C. de Q.
245 $aIn vivo and in vitro growth of immature embryos of peach (Prunus persica (L.) Batsch).
260 $a[College Station] : Texas A & M University$c1992
300 $a95p.
500 $aPhD. Thesis.
520 $aMedia and novel in vitro manipulation and techniques may improve growth of immature (< 10 mm ) peach embryos. The objectives of thisw study were to evaluate a) in vivo growth of fruts and seed parts and nutriente contents of the developing peach embryos, b) in ovelo growth and quality of immature embryos ( 8.7 mm) as influenced by media (MSM or SHM), sucrose levels (6% or 10%), ovule support systems (vermiculite, VS; saolid Gelrite, GS; filter paper, FPS), and c) in ovulo growth and nutrient contents of small embryos (<5 mm ) as influenced by cultural techniques (intact and perforated oveles, or embryo nurse), ovule support system (VS or semi- solid agar, SSA), media (SHM or Monnier), Hormones (0, 0.25, 050 mg.l-1 LAA plus 0.01 mg.l-1 kinetin), and L-glutamine (none or 400 mg. l-1). In vivo growth trends among cultivars varied in response to the temperature and fruiting set differences between the years. Higher mean temperatura and low fruit set promoted faster embryo growth. Fruit fresh weight, ovele length, and ovule fresh wieght showed low and inconsistent correlations with embryo length and embryo dry weight; they could not be used as a nondestructive method for embryo selection in the field. In vivo embryos had higher macronutrient concentrations than in vitro embryos. However, in vitro embryos showed higher dry matter (24%) as compared to in vivo embryos (12%) which may express a batter quality embryos. Embryos from ovule perforation treatments showed lower nutrient concentrations than those from intact ovules, although their growth was higher in length and dry weight. The ovule support system influenced embruo (8.7 mm) growth more then media or sucrose. The VS system promoted two to four-fold higher embryo dry weight FPS or GS, basides being easier to set up and handle than FPS or GS suystem. The effect of sucrose varied with the medium used; maxsimum embryo growth occurred winth 10% sucrose (w/v) on either SHM or MSM medimum, although SHM at 6% sucrose promoted equal growth as SHM with 10% sucrose. The SHM medium was as effective as Monnier medium on growth of small (<5 mm) embryos, but their effectiveness depended on the technique and ovule support system used, Neither SHM nor Monnier medium was effective on Goldprince embryo growteh (length) when the embryo nurse (EN) technique was used. THE ovule perfotion (OP) and VS system cvonsistently promoted 60 to 100% more embryo growth the conventional intact ovules and the EN techniques. Monnier medium, 12%sucrose, with OP/VS conbination promoted four to two-fold higher embryo dry weight of P51-2 and Goldcrest peaches than OP plus semi-solid agar support (SSA). The monnier medium with 12% sucrose, and OP/VS increased Sprigcrestembryos (2.3 mm) up to 9.6 mm in 30 days (1mm of growth per each 5 days in culture). The effect amino acid L-glutamine varied with genotypes, embryo age, and hormones. A negative effect on growth of Golgprince embryoa (4 mm) occurred whed L-glutamine was used witeh SHM but for Ear ligrandes 1.5 mm embryos a growth effect was found. Without hormones, the SHM medium + L glutamine promoted greater ovule and embryo growth than SHM plus homones.Finally, for an avarege of 35% seedling recovery rate the following sbase line growth criterion is needed: FDP > 75 days, B) ovule dry weight 38 to 96 mg (mean of 51 mg), c) PDW of ovule between 10% and 28% (mean 17%), d) embryo length > 10 mm, and e) PF1 >60%.
650 $agermination
650 $aCrescimento
650 $aEmbrião
650 $aGerminação
650 $aPêssego
650 $aPrunus Persica
650 $aSemente
653 $aGrowth
653 $aPeach
653 $aPlant embryo
653 $aSeed
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