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Registro Completo |
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
30/03/2016 |
Data da última atualização: |
30/03/2016 |
Autoria: |
SANTOS, S. B.; PINHEIRO-JÚNIOR, J. W.; MOTA, A. R.; SANTOS, A. S.; ALVES, B. H. L. S.; OLIVEIRA, J. M. B.; SILVA, L. B. G.; MOTA, R. A. |
Afiliação: |
SANDRA B. SANTOS, UFRPE; JOSÉ W. PINHEIRO-JUNIOR, UFRPE; ANDRÉ R. MOTA, UFRPE; ANDRÉ S. SANTOS, UFRPE; BRUNO H. L. S. ALVES, UFRPE; JÚNIOR M. B. OLIVEIRA, UFRPE; LEONILDO B. G. SILVA, UFRPE; RINALDO A. MOTA, UFRPE. |
Título: |
Recovery of Molicutes from the reproductive tract of dairy cattle in the state of Pernambuco, Brazil. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 6, p. 491-496, jun. 2015. |
Idioma: |
Inglês |
Conteúdo: |
The aim of the present study was to report the occurrence of members of the Mollicutes class in the reproductive system of dairy cattle in Brazil. Five farms containing dairy cattle were visited in January of 2012. In total, 100 cows of different ages, breeds and stages of lactation were examined in the present study. The cows were part of intensive or semi-intensive management systems and were submitted to mechanical milking or hand milking. The samples were collected after washing the vulvar region with water and soap, and then drying it with paper towels and disinfecting the area with alcohol (70°GL). Vaginal mucous was collected using a sterile alginate cotton swab, which was rubbed on the vagina, as well as the lateral and internal walls. Vulvovaginal mucous samples were cultured in both liquid and solid modified Hayflick´s medium, for mycoplasmas, and UB medium, for ureaplasmas. The PCR assays for Mollicutes and Ureaplasma spp. were performed according to the standard protocols described in the current literature. During isolation, the frequency of Mycoplasma spp. was of 13.0% (13/100) and for Ureaplasma spp. was of 6.0% (6/100). In the PCR assays the frequency of Mollicutes was of 26.0% (26/100) and for Ureaplasma spp. was of 13.0% (13/100) in the dairy cattle studied. This is the first report of these agents in reproductive system of bovine of the Pernambuco state. Further studies are necessary to determine the pathogenic potential and species of these field isolates. |
Palavras-Chave: |
Muco vaginal; Reproduction diseases; Ureaplasma spp; Vaginal mucous. |
Thesagro: |
Micoplasmose; Vaca leiteira. |
Thesaurus Nal: |
Dairy cows; Mycoplasmosis. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141803/1/Recover-of-mollicutes.pdf
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Marc: |
LEADER 02384naa a2200301 a 4500 001 2042162 005 2016-03-30 008 2015 bl uuuu u00u1 u #d 100 1 $aSANTOS, S. B. 245 $aRecovery of Molicutes from the reproductive tract of dairy cattle in the state of Pernambuco, Brazil. 260 $c2015 520 $aThe aim of the present study was to report the occurrence of members of the Mollicutes class in the reproductive system of dairy cattle in Brazil. Five farms containing dairy cattle were visited in January of 2012. In total, 100 cows of different ages, breeds and stages of lactation were examined in the present study. The cows were part of intensive or semi-intensive management systems and were submitted to mechanical milking or hand milking. The samples were collected after washing the vulvar region with water and soap, and then drying it with paper towels and disinfecting the area with alcohol (70°GL). Vaginal mucous was collected using a sterile alginate cotton swab, which was rubbed on the vagina, as well as the lateral and internal walls. Vulvovaginal mucous samples were cultured in both liquid and solid modified Hayflick´s medium, for mycoplasmas, and UB medium, for ureaplasmas. The PCR assays for Mollicutes and Ureaplasma spp. were performed according to the standard protocols described in the current literature. During isolation, the frequency of Mycoplasma spp. was of 13.0% (13/100) and for Ureaplasma spp. was of 6.0% (6/100). In the PCR assays the frequency of Mollicutes was of 26.0% (26/100) and for Ureaplasma spp. was of 13.0% (13/100) in the dairy cattle studied. This is the first report of these agents in reproductive system of bovine of the Pernambuco state. Further studies are necessary to determine the pathogenic potential and species of these field isolates. 650 $aDairy cows 650 $aMycoplasmosis 650 $aMicoplasmose 650 $aVaca leiteira 653 $aMuco vaginal 653 $aReproduction diseases 653 $aUreaplasma spp 653 $aVaginal mucous 700 1 $aPINHEIRO-JÚNIOR, J. W. 700 1 $aMOTA, A. R. 700 1 $aSANTOS, A. S. 700 1 $aALVES, B. H. L. S. 700 1 $aOLIVEIRA, J. M. B. 700 1 $aSILVA, L. B. G. 700 1 $aMOTA, R. A. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 6, p. 491-496, jun. 2015.
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Embrapa Unidades Centrais (AI-SEDE) |
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Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
28/09/2012 |
Data da última atualização: |
12/06/2015 |
Tipo da produção científica: |
Documentos |
Autoria: |
ZANELA, M. B.; RIBEIRO, M. E. R.; KOLLING, G. J. |
Afiliação: |
MAIRA BALBINOTTI ZANELA, CPACT; MARIA EDI ROCHA RIBEIRO, CPACT; Giovani Jacob Kolling, Médico-veterinário, Mestrando, Estagiário da Embrapa Clima Temperado. |
Título: |
Manejo de ordenha. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Pelotas: Embrapa Clima Temperado, 2011. |
Páginas: |
22 p. |
Série: |
(Embrapa Clima Temperado. Documentos, 342.) |
ISBN: |
1516-8840 |
Idioma: |
Português |
Palavras-Chave: |
Leite - Produção; Manejo Animal. |
Thesagro: |
Ordenha. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67059/1/Documento-342.pdf
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Marc: |
LEADER 00504nam a2200193 a 4500 001 1934943 005 2015-06-12 008 2011 bl uuuu u0uu1 u #d 022 $a1516-8840 100 1 $aZANELA, M. B. 245 $aManejo de ordenha. 260 $aPelotas: Embrapa Clima Temperado$c2011 300 $a22 p. 490 $a(Embrapa Clima Temperado. Documentos, 342.) 650 $aOrdenha 653 $aLeite - Produção 653 $aManejo Animal 700 1 $aRIBEIRO, M. E. R. 700 1 $aKOLLING, G. J.
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