Data/hora: 20/06/2024 11:40:08

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 07/11/2022
Data da última atualização: | 07/11/2022
Autoria: | PINHEIRO, P. V.; WILSON, J. R.; XU, Y.; ZHENG, Y.; REBELO, A. R.; FATTAH-HOSSEINI, S.; KRUSE, A.; SILVA, R. S. dos; XU, Y.; KRAMER, M.; GIOVANNONI, A.; FEI, Z.; GRAY, S.; HECK, M.
Afiliação: | PATRICIA VALLE PINHEIRO, CNPAF; JENNIFER R. WILSON, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; YI XU, CORNELL UNIVERSITY; YI ZHENG, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; ANA RITA REBELO, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; SOMAYEH FATTAH-HOSSEINI, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; ANGELA KRUSE, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; ROGERIO SANTOS DOS SILVA, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; YIMIN XU, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; MATTHEW KRAMER, USDA; AMES GIOVANNONI, CORNELL UNIVERSITY; ZHANGJUN FEI, BOYCE THOMPSON INSTITUTE FOR PLANT RESEARCH; STEWART GRAY, CORNELL UNIVERSITY; MICHELLE HECK, CORNELL UNIVERSITY.
Título: | Plant viruses transmitted in two different modes produce differing effects on small RNA-Mediated processes in their aphid vector.
Ano de publicação: | 2019
Fonte/Imprenta: | Phytobiomes Journal, v. 3, n. 1, p. 71-81, 2019.
ISSN: | 2471-2906
DOI: | https://doi.org/10.1094/PBIOMES-10-18-0045-R
Idioma: | Inglês
Conteúdo: | Transmission of plant viruses by aphids involves multitrophic interactions among host plants, aphid vectors, and plant viruses. Here, we used small RNA (sRNA) sequencing to visualize the sRNA response of Myzus persicae to two plant viruses that M. persicae transmits in different modes: the nonpersistent Potato virus Y (PVY) versus the persistent Potato leafroll virus (PLRV). Aphids exposed to PLRV produced significantly less 22 mers aligned to the aphid genome, and an abundance of 26 to 27 mers, many of which were predicted to be piRNA. Additionally, expression of Buchnera aphidicola tRNA-derived sRNAs was influenced by PLRV and, to a lesser extent, PVY, suggesting that plant viruses alter the aphid-endosymbiont relationship. Finally, aphids exposed to PLRV-infected plants generated an abundance of unusually long sRNAs and a reduced number of 22 mers against an aphid virus, Myzus persicae densovirus (MpDNV) and had higher MpDNV titer. Expression of the PLRV silencing suppressor P0 in plants recapitulated the increase in MpDNV titer in the absence of PLRV infection. Our results show that plant viruses transmitted in two different modes cause distinct effects on their vector with regards to post-transcriptional gene regulation, symbiosis with Buchnera, and the antiviral immune response of aphids to an aphid-infecting densovirus.
Palavras-Chave: | Aphid vector; RNA-mediated.
Thesagro: | Myzus Persicae; RNA; Vírus.
Thesaurus Nal: | Buchnera aphidicola; Plant viruses; Potato leafroll virus.
Categoria do assunto: | O Insetos e Entomologia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (36545-1) - UPC - AP - DD - 2019 -  - 2019 - DIS