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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/07/2004 |
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Data da última atualização: |
26/07/2017 |
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Autoria: |
PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M. |
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Título: |
Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Biological Control, v. 27, n. 3, p. 336-344, July 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Some baculovirus have been genetically modified for the inactivation of their ecdysteroid
glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type
AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A.
gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. |
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Palavras-Chave: |
Ecdysteroid glucosyltransferase; Velvetbean caterpillar. |
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Thesagro: |
Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética. |
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Categoria do assunto: |
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Marc: |
LEADER 02224naa a2200241 a 4500
001 1467192
005 2017-07-26
008 2003 bl --- 0-- u #d
100 1 $aPINEDO, F. J. R.
245 $aInactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host.
260 $c2003
520 $aSome baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV.
650 $aAnticarsia Gemmatalis
650 $aBaculovirus
650 $aControle Biológico
650 $aEngenharia Genética
653 $aEcdysteroid glucosyltransferase
653 $aVelvetbean caterpillar
700 1 $aMOSCARDI, F.
700 1 $aLUQUE, T.
700 1 $aOLSZEWSKI, J. A.
700 1 $aRIBEIRO, B. M.
773 $tBiological Control$gv. 27, n. 3, p. 336-344, July 2003.
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Embrapa Soja (CNPSO) |
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| 1. |  | POLIDORO, J. C.; MENDONÇA-SANTOS, M. de L.; LUMBRERAS, J. F.; COELHO, M. R.; CARVALHO FILHO, A. de; MOTTA, P. E. F. da; CARVALHO JUNIOR, W. de; ARAUJO FILHO, J. C. de; CURCIO, G. R.; CORREIA, J. R.; MARTINS, E. de S.; SPERA, S. T.; OLIVEIRA, S. R. de M.; BOLFE, E. L.; MANZATTO, C. V.; TOSTO, S. G.; VENTURIERI, A.; SA, I. B.; OLIVEIRA, V. A. de; SHINZATO, E.; ANJOS, L. H. C. dos; VALLADARES, G. S.; RIBEIRO, J. L.; MEDEIROS, P. S. C. de; MOREIRA, F. M. de S.; SILVA, L. S. L.; SEQUINATTO, L.; AGLIO, M. L. D.; DART, R. de O. Programa Nacional de Solos do Brasil (PronaSolos). Rio de Janeiro: Embrapa Solos, 2016. 53 p. (Embrapa Solos. Documentos, 183).| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Amazônia Oriental; Embrapa Cocais; Embrapa Florestas; Embrapa Meio Ambiente; Embrapa Semiárido; Embrapa Solos. |
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