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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
28/02/2008 |
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Data da última atualização: |
07/02/2020 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
PACHECO, L. R. F.; MARIN, F. R.; PILAU, F. G.; LOPES-ASSAD, M. L.; PINTO, H. S.; CONCEIÇÃO, M. A. F.; TONIETO, J.; MANDELLI, F. |
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Afiliação: |
LUDMILA R. F. PACHECO, Estagiária do CNPTIA; FÁBIO RICARDO MARIN, CNPTIA; FELIPE G. PILAU, CNPTIA; MARIA L. LOPES ASSAD, UFSCar; HILTON S. PINTO, Cepagri/Unicamp; MARCO ANTONIO FONSECA CONCEICAO, CNPUV; JORGE TONIETO, CNPUV; FRANCISCO MANDELLI, CNPUV. |
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Título: |
Potencial climático para produção de vinhos de qualidade no Estado de São Paulo. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 15., 2007, Aracaju. Efeito das mudanças climáticas na agricultura: anais. Aracajú: SBA: Embrapa Tabuleiros Costeiros, 2007. Não paginado. |
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Idioma: |
Português |
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Conteúdo: |
Considerando este contexto, este trabalho objetivou estudar o potencial climático da vitivinicultura de qualidade no estado de São Paulo, utilizando o sistema de classificação climática multicritério para a regionalização dos grupos climáticos predominantes no Estado de São Paulo. |
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Palavras-Chave: |
Geoprocessamento; Geoprocessing; Grape crop; Zoneamento. |
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Thesagro: |
Uva; Viticultura. |
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Thesaurus Nal: |
zoning. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/159594/1/PL-Potencial-Pacheco-CBA-2007.pdf
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Marc: |
LEADER 01203nam a2200277 a 4500
001 1004779
005 2020-02-07
008 2007 bl uuuu u00u1 u #d
100 1 $aPACHECO, L. R. F.
245 $aPotencial climático para produção de vinhos de qualidade no Estado de São Paulo.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 15., 2007, Aracaju. Efeito das mudanças climáticas na agricultura: anais. Aracajú: SBA: Embrapa Tabuleiros Costeiros, 2007. Não paginado.$c2007
520 $aConsiderando este contexto, este trabalho objetivou estudar o potencial climático da vitivinicultura de qualidade no estado de São Paulo, utilizando o sistema de classificação climática multicritério para a regionalização dos grupos climáticos predominantes no Estado de São Paulo.
650 $azoning
650 $aUva
650 $aViticultura
653 $aGeoprocessamento
653 $aGeoprocessing
653 $aGrape crop
653 $aZoneamento
700 1 $aMARIN, F. R.
700 1 $aPILAU, F. G.
700 1 $aLOPES-ASSAD, M. L.
700 1 $aPINTO, H. S.
700 1 $aCONCEIÇÃO, M. A. F.
700 1 $aTONIETO, J.
700 1 $aMANDELLI, F.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
29/05/2023 |
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Data da última atualização: |
23/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MOREIRA, V. J. V.; PINHEIRO, D. H.; LOURENCO, I. T.; BASSO, M. F.; LISEI-DE-SA, M. E.; SILVA, M. C. M. da; DANCHIN, E. G. J.; GUIMARAES, P. M.; GRYNBERG, P.; BRASILEIRO, A. C. M.; MACEDO, L. L. P. de; MORGANTE, C. V.; ENGLER, J. de A.; SA, M. F. G. de. |
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Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, Biotechnology Center, PPGBCM, UFRGS; DANIELE HELOÍSA PINHEIRO, National Institute of Science and Technology; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology; MARIA E. LISEI-DE-SA, National Institute of Science and Technology; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ETIENNE G. J. DANCHIN, National Institute of Science and Technology; PATRICIA MESSEMBERG GUIMARAES, Cenargen; PRISCILA GRYNBERG, Cenargen; ANA CRISTINA MIRANDA BRASILEIRO, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal of Pest Science, v. 97, p. 411-427, 2024. |
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DOI: |
https://doi.org/10.1007/s10340-023-01623-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Meloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. MenosMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disor... Mostrar Tudo |
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Palavras-Chave: |
DsRNA; Host-induced gene silencing; Interações planta-nematóide; Nematoide das galhas; Plant-nematode interactions; Root-knot nematode. |
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Thesagro: |
Meloidogyne Incognita; Nematóide; Parasito de Planta; Planta. |
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Thesaurus NAL: |
Nematode biology; Nematode control; Plant parasitic nematodes. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/264479/1/In-planta-RNAi-targeting-Meloidogyne-incognita-Minc16803-gene-perturbs-nematode-parasitism-and-reduces-plant-susceptibility.pdf
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Marc: |
LEADER 03269naa a2200445 a 4500
001 2154064
005 2024-05-23
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10340-023-01623-7$2DOI
100 1 $aMOREIRA, V. J. V.
245 $aIn planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility.$h[electronic resource]
260 $c2024
520 $aMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing.
650 $aNematode biology
650 $aNematode control
650 $aPlant parasitic nematodes
650 $aMeloidogyne Incognita
650 $aNematóide
650 $aParasito de Planta
650 $aPlanta
653 $aDsRNA
653 $aHost-induced gene silencing
653 $aInterações planta-nematóide
653 $aNematoide das galhas
653 $aPlant-nematode interactions
653 $aRoot-knot nematode
700 1 $aPINHEIRO, D. H.
700 1 $aLOURENCO, I. T.
700 1 $aBASSO, M. F.
700 1 $aLISEI-DE-SA, M. E.
700 1 $aSILVA, M. C. M. da
700 1 $aDANCHIN, E. G. J.
700 1 $aGUIMARAES, P. M.
700 1 $aGRYNBERG, P.
700 1 $aBRASILEIRO, A. C. M.
700 1 $aMACEDO, L. L. P. de
700 1 $aMORGANTE, C. V.
700 1 $aENGLER, J. de A.
700 1 $aSA, M. F. G. de
773 $tJournal of Pest Science$gv. 97, p. 411-427, 2024.
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Embrapa Semiárido (CPATSA) |
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