Data/hora: 22/06/2024 10:32:28

Biblioteca(s): 	Embrapa Gado de Leite; Embrapa Rondônia.	
Data corrente: 	03/04/2012	
Data da última atualização: 	10/11/2021	
Autoria: 	PFEIFER, L. F. M.; SIQUEIRA, L. G. B.; ADAMS, G. P.; PIERSON, R. A.; SINGH, J.	
Afiliação: 	LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; G.P. Adams, Department of Veterinary Biomedical Sciences, University of Saskatchewan; R.A. Pierson, Department of Obstetrics, Gynecology and Reproductive Sciences, University of Saskatchewan; J. Singh, Department of Veterinary Biomedical Sciences, University of Saskatchewan.	
Título: 	In vivo imaging of cumulus-oocyte-complexes and small ovarian follicles in cattle using ultrasonic biomicroscopy.	
Ano de publicação: 	2012	
Fonte/Imprenta: 	Animal Reproduction Science, Amsterdam, v. 131, p. 88-94, 2012.	
ISSN: 	0378-4320	
Idioma: 	Inglês	
Notas: 	doi:10.1016/j.anireprosci.2012.02.014	
Conteúdo: 	The objectives of this study were to: (1) evaluate the feasibility of ultrasonic biomicroscopy (UBM) for imaging ovarian follicles in vivo in cows and heifers, (2) compare the transvaginal to the transrectal approach, (3) compare the number of follicles detected by UBM to conventional ultrasonography (US), and (4) identify cumulus-oocyte-complexes (COC) within follicles by UBM. Mature cows (n = 5) and peri-pubertal heifers (n = 5) were examined once using conventional B-mode US (Aloka 900) with a 7.5 MHz transrectal, and a 5 MHz transvaginal transducers. A second series of examinations was performed using UBM (Visualsonics Vevo 660) equipped with a 30 MHz transrectal and a 40 MHz transvaginal transducer. A three- to four-fold increase in the number of small follicles (<3 mm) was detected using the transvaginal approach with UBM compared to conventional US in both heifers (32.4 ± 4.24 compared to 7.2 ± 1.4; P < 0.0001) and cows (35.0 ± 13.8 compared to 10.7 ± 7.5; P = 0.0013). However, fewer follicles (all sizes combined) were detected using the transrectal approach with UBM compared to conventional US in both heifers (8.6 ± 5.6 compared to 17.6 ± 4.9; P = 0.02) and cows (5.3 ± 6.1 compared to 20.3 ± 7; P = 0.04). In heifers, COC-like structures were identified in 39 of 202 (19.3%) follicles examined. In conclusion, UBM using a transvaginal approach is feasible and may be used for in vivo assessment of early antral follicles as small as 0.4 mm, and COC within follicles.	
Palavras-Chave: 	Biomicroscope; Biomicroscopia; Biomicroscopico; Foliculos ovarianos; Imagem; Imaging; Oocito; Oocyte; Ultrasonografia animal; Ultrassonografia.	
Thesaurus Nal: 	ovarian follicles; ultrasonography.	
Categoria do assunto: 	G Melhoramento Genético
L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Rondônia (CPAF-RO)	
Exemplares: 	CPAF-RO - (15815-1) - UPC - AP - DD -  -  -  - DIS
CNPGL - (19570-1) - UPC - AP - DD -  -  -  - DIS