Data/hora: 08/06/2024 20:39:38

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 19/08/2005
Data da última atualização: | 06/11/2006
Autoria: | PICOLI, E. A. de T.; ALFENAS, A. C.; GONÇALVES, R. C.; DIAS, L. L. C.; NEVES, D. A.; OTONI, W. C.; ROMEIRO, R. da S.
Título: | Detection and antibiotic treatment of Herbaspirillum huttiense isolated from in vitro explants of Eucalyptus sp.
Ano de publicação: | 2005
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 5, n. 2, p. 191-198, June 2005.
Idioma: | Inglês
Conteúdo: | In vitro assays involving explant cultures aiming to establish a genetic transformation protocol for the Eucalyptus grandis vs. E. smithii hybrid were hampered by the contamination of the explants. One yeast and one bacterium were isolated. The bacterium was identified as Herbaspirillum huttiense, with which further analyses were carried out and its response to different antibiotics were evaluated. Amongst 34 in vitro tested antibiotics, 12 inhibited the bacterial growth. Sulfadiazine, streptomycin, kanamycin and penicillin were selected for determination of the minimal inhibitory concentration. Sulfadiazine, streptomycin and kanamycin presented inhibition halos at 256 and 512 mg l-1, while penicillin did not display bacteriostatic effects in concentrations up to 1024 mg l-1.
Palavras-Chave: | Cultura de tecidos vegetais; Endófita; Endophyte; Plant tissue culture.
Thesagro: | Contaminação Bacteriana; Eucalipto.
Thesaurus Nal: | bacterial contamination; Eucalyptus.
Categoria do assunto: | --
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (22906-1) - ADD - AP - -- - 631.05 - C - 2005.00379 - DIS