Data/hora: 22/09/2024 12:07:54

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	02/10/2013	
Data da última atualização: 	02/10/2013	
Autoria: 	PETTA, T.; RAICHARDT, L.; MELO, I. S. de; MORAES, L. A. B.	
Afiliação: 	TÂNIA PETTA, FFCLRP-USP; LEANDRO RAICHARDT, FFCLRP-USP; ITAMAR SOARES DE MELO, CNPMA; LUIZ A. B. MORAES, FFCLRP-USP.	
Título: 	Bioassay-guided isolation of a low molecular weight PHB from Burkholderia sp. with phytotoxic activity.	
Ano de publicação: 	2013	
Fonte/Imprenta: 	Applied Biochemistry and Biotechnology, Totowa, v. 170, n. 7, p. 1689-1701, 2013.	
Idioma: 	Inglês	
Conteúdo: 	This work reports on the bioassay-guided isolation and identification of themacrocyclic pentolide 1, a cyclic polyhydroxybutyrate (PHB) with low molecularweight. This metabolite is produced by Burkholderia sp. and it exhibited phytotoxicactivity in a Lemna minor bioassay. Its structure was determined by 1H and 13CNMR, heteronuclear multiple quantum correlation, heteronuclear multiple bond correlation, IR, and electrospray ionization tandem mass spectrometry analyses. The period for maximum production of the pentolide was optimized and determined on the basis of multiple reaction monitoring experiments at 15 days. The potential of Burkholderia sp. as a producer of higher biopolymers of PHB was also investigated. The methodology employed here accelerated the isolation and characterization of a phytotoxicmetabolite whose structure can serve as a model for the synthesis of new classes of herbicides.	
Palavras-Chave: 	Bioassay; Burkholderia sp; LC-MS; Liquid chromatography-mass spectrometry; Polihidroxibutirato; Polímero.	
Thesagro: 	Bactéria; Herbicida; Toxidez.	
Thesaurus Nal: 	Lemna minor; phytotoxicity; polyhydroxybutyrate.	
Categoria do assunto: 	S Ciências Biológicas	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (12798-1) - UPC - AP - DD -  -  -  - DIS