| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Hortaliças; Embrapa Instrumentação. |
|
Data corrente: |
18/11/2021 |
|
Data da última atualização: |
23/01/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PETRÔNIO, M. S.; BARROS-ALEXANDRINO, T. T.; LIMA, A. M. F.; ASSIS, O. B. G. de; INOUE-NAGATA, A. K.; NAKASU, E. Y. T.; TIERA, M. J.; PILON, L. |
|
Afiliação: |
ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; ALICE KAZUKO INOUE NAGATA, CNPH; ERICH YUKIO TEMPEL NAKASU, CNPH; LUCIMEIRE PILON, CNPH. |
|
Título: |
Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022. |
|
Páginas: |
5266 - 5279 |
|
DOI: |
https://doi.org/10.33263/BRIAC124.52665279 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Technologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection. |
|
Palavras-Chave: |
Chitosan nanocarrier; DsRNA phytotoxicity assay; Tomato mosaic virus (ToMV). |
|
Thesaurus Nal: |
Double-stranded RNA; Lettuce; RNA interference; Tomato mosaic virus. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232647/1/P-Physicochemical-and-Toxicity-Investigation-of-Chitosanbased-dsRNA-Nanocarrier-Formation.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227886/1/20695837124.526652791.pdf
|
|
Marc: |
LEADER 02428naa a2200313 a 4500
001 2141015
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.33263/BRIAC124.52665279$2DOI
100 1 $aPETRÔNIO, M. S.
245 $aPhysicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation.$h[electronic resource]
260 $c2022
300 $a5266 - 5279
520 $aTechnologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection.
650 $aDouble-stranded RNA
650 $aLettuce
650 $aRNA interference
650 $aTomato mosaic virus
653 $aChitosan nanocarrier
653 $aDsRNA phytotoxicity assay
653 $aTomato mosaic virus (ToMV)
700 1 $aBARROS-ALEXANDRINO, T. T.
700 1 $aLIMA, A. M. F.
700 1 $aASSIS, O. B. G. de
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAKASU, E. Y. T.
700 1 $aTIERA, M. J.
700 1 $aPILON, L.
773 $tBiointerface Research in Applied Chemistry, vol. 12$gn. 4, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Instrumentação (CNPDIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 3 | |
| 1. |  | VARGAS, J. R.; SOUSA, B. M. C. de; SILVA, D. Y. M.; PETRONIO, M. S.; NAKASU, E. Y. T.; INOUE-NAGATA, A. K. Latest advances in the use of double stranded RNA as a tool for viral protection in tomato plants. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais 2023. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 670 Resumo.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
|    |
| 2. | | PETRÔNIO, M. S.; BARROS-ALEXANDRINO, T. T.; LIMA, A. M. F.; ASSIS, O. B. G. de; INOUE-NAGATA, A. K.; NAKASU, E. Y. T.; TIERA, M. J.; PILON, L. Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022. 5266 - 5279| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Instrumentação. |
| |
| 3. | | VARGAS, J. R.; SOUSA, B. M. C. de; SILVA, D. Y. M.; MARTINS, Y. F.; PEREIRA, W. de S.; PETRONIO, M. S.; NAKASU, E. Y. T.; INOUE-NAGATA, A. K. Últimos avanços no uso de RNA de fita dupla como ferramenta para proteção viral em plantas de tomate. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., Brasília, DF, 2023. Anais...Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 670.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Hortaliças. |
| |
| Registros recuperados : 3 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
