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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças; Embrapa Instrumentação. |
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Data corrente: |
18/11/2021 |
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Data da última atualização: |
23/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PETRÔNIO, M. S.; BARROS-ALEXANDRINO, T. T.; LIMA, A. M. F.; ASSIS, O. B. G. de; INOUE-NAGATA, A. K.; NAKASU, E. Y. T.; TIERA, M. J.; PILON, L. |
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Afiliação: |
ODILIO BENEDITO GARRIDO DE ASSIS, CNPDIA; ALICE KAZUKO INOUE NAGATA, CNPH; ERICH YUKIO TEMPEL NAKASU, CNPH; LUCIMEIRE PILON, CNPH. |
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Título: |
Physicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Biointerface Research in Applied Chemistry, vol. 12, n. 4, 2022. |
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Páginas: |
5266 - 5279 |
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DOI: |
https://doi.org/10.33263/BRIAC124.52665279 |
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Idioma: |
Inglês |
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Conteúdo: |
Technologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection. |
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Palavras-Chave: |
Chitosan nanocarrier; DsRNA phytotoxicity assay; Tomato mosaic virus (ToMV). |
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Thesaurus Nal: |
Double-stranded RNA; Lettuce; RNA interference; Tomato mosaic virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/232647/1/P-Physicochemical-and-Toxicity-Investigation-of-Chitosanbased-dsRNA-Nanocarrier-Formation.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227886/1/20695837124.526652791.pdf
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Marc: |
LEADER 02428naa a2200313 a 4500
001 2141015
005 2024-01-23
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.33263/BRIAC124.52665279$2DOI
100 1 $aPETRÔNIO, M. S.
245 $aPhysicochemical and toxicity investigation of chitosan-based dsRNA nanocarrier formation.$h[electronic resource]
260 $c2022
300 $a5266 - 5279
520 $aTechnologies involving the use of double-stranded RNA (dsRNA) to elicit RNA interference (RNAi) in pest control have emerged as an alternative to traditional pesticides. RNAi can mediate natural cell protection being a promising tool to provide prompt responses in plant defense against pathogens. The present study is focused on the physicochemical characterization of formed dsRNAloaded nanoparticles as a result of chitosan-dsRNA ionic interactions. Additionally, a preliminary investigation was conducted of the in-vitro toxicity of loaded nanoparticles in lettuce and human red blood cells. dsRNA molecules, homologous to partial phytopathogenic tomato mosaic virus (ToMV) sequence, were used as a model. The main groups involved in the chitosan-dsRNA ionic coupling were identified by Fourier-transform infrared spectroscopy, and the stability of formed nanoparticles was accessed by dynamic light scattering, electrophoresis, and thermal analyses. The chitosan showed a higher ability to bind to dsRNA at low charge ratios (N/P = 1), ruled by positively charged chitosan methyl groups and negatively charged phosphate groups from the RNA backbone, resulting in small nanoparticles (73.25 nm size) at low polydispersity (0.25). The toxic assays of these particles, on lettuce seeds and in human erythrocytes, revealed very low toxicity demonstrating their safety as a platform, thereby holding potential use as biodefensive for crop protection.
650 $aDouble-stranded RNA
650 $aLettuce
650 $aRNA interference
650 $aTomato mosaic virus
653 $aChitosan nanocarrier
653 $aDsRNA phytotoxicity assay
653 $aTomato mosaic virus (ToMV)
700 1 $aBARROS-ALEXANDRINO, T. T.
700 1 $aLIMA, A. M. F.
700 1 $aASSIS, O. B. G. de
700 1 $aINOUE-NAGATA, A. K.
700 1 $aNAKASU, E. Y. T.
700 1 $aTIERA, M. J.
700 1 $aPILON, L.
773 $tBiointerface Research in Applied Chemistry, vol. 12$gn. 4, 2022.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/02/2009 |
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Data da última atualização: |
09/05/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RAMOS, V. R.; GALHARDO, I. C.; CASTRO, R. C. S.; GUIMARÃES, P. M.; BERTIOLI, D. J.; MORETZSOHN, M. C.; LEAL BERTIOLI, S. C. M. |
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Afiliação: |
VALÉRIA ROCHA RAMOS; I. C. GALHARDO, UNB; RODRIGO CESAR DA SILVA CASTRO; PATRÍCIA MESSEMBERG GUIMARÃES, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; DAVID J. BERTIOLI, UCB; MÁRCIO DE CARVALHO MORETZSOHN, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA; SORAYA CRISTINA DE MACEDO LEAL BERTIOLI, EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA. |
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Título: |
Fenotipagem para resistência a ferrugem em uma prpulação F3 segregante de Arachis hypogaea e um anfidiplóide sintético, visando à identificação de QTLs. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. |
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Páginas: |
p. 82. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Palavras-Chave: |
Espécie silvestre; Pré-melhoramento. |
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Thesagro: |
Amendoim; Ferrugem. |
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Thesaurus NAL: |
Arachis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00817nam a2200241 a 4500
001 1190686
005 2024-05-09
008 2008 bl uuuu u01u1 u #d
100 1 $aRAMOS, V. R.
245 $aFenotipagem para resistência a ferrugem em uma prpulação F3 segregante de Arachis hypogaea e um anfidiplóide sintético, visando à identificação de QTLs.
260 $aIn: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG$c2008
300 $ap. 82.$c1 CD-ROM.
650 $aArachis
650 $aAmendoim
650 $aFerrugem
653 $aEspécie silvestre
653 $aPré-melhoramento
700 1 $aGALHARDO, I. C.
700 1 $aCASTRO, R. C. S.
700 1 $aGUIMARÃES, P. M.
700 1 $aBERTIOLI, D. J.
700 1 $aMORETZSOHN, M. C.
700 1 $aLEAL BERTIOLI, S. C. M.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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