Data/hora: 25/06/2024 14:26:58

Biblioteca(s): 	Embrapa Gado de Leite.	
Data corrente: 	17/03/2011	
Data da última atualização: 	15/02/2024	
Autoria: 	POLISSENI, J.; SÁ, W. F. de; GUERRA, M. O.; MACHADO, M. A.; SERAPIÃO, R. V.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; PETERS, V. M.	
Afiliação: 	JULIANA POLISSENI, UFJF; WANDERLEI FERREIRA DE SÁ, Pesquisador aposentado do CNPGL; MARTHA DE OLIVEIRA GUERRA, UFJF; MARCO ANTONIO MACHADO, CNPGL; RAQUEL VARELLA SERAPIÃO, UFJF; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF.	
Título: 	Post biopsy bovine embryo viability and whole genome amplification in preimplantation genetic diagnosis.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	Fertility and Sterility, v. 93. n. 3, p. 783-788, 2010.	
DOI: 	https://doi.org/10.1016/j.fertnstert.2008.10.023	
Idioma: 	Inglês	
Conteúdo: 	Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.Objective: To evaluate the effect of the biopsy of 8-cell to 16-cell bovine embryos on their subsequent development and the effect of whole genome amplification (WGA) on removed blastomeres. Design: Randomized study. Setting: Molecular genetics and animal reproduction laboratories. Patient(s): Cow ovaries obtained from slaughterhouses. Intervention(s): The ovaries were punctured, and the oocytes were matured and fertilized in vitro. On the fourth day after fertilization, 8-cell to 16-cell bovine embryos were biopsied, one quarter of each embryo being removed. The blastomeres were submitted to WGA followed by polymerase chain reaction (PCR). The embryos were returned to culture for evaluation of their development. Main outcome measure(s): Subsequent rate of blastocyst development, embryo cell number, WGA efficiency, and sex determination. Result(s): A total of 92 embryos were submitted to biopsy. The blastocyst production was 53.3%, with 44.9% of hatching rate. These results were similar to those of the control group (66.0% and 42.6%) of 103 embryos. Overall, no impact was detected on embryo quality in blastocyst cell number between the two groups. Removed blastomeres were submitted to WGA, resulting in 98.2% of efficiency. However, only 59% of the samples were sexed by PCR. Conclusion(s): Biopsy of 8-cell to 16-cell bovine embryos did not affect their subsequent development. WGA was successful in removed blastomeres.	
Palavras-Chave: 	Embryo quality; Hatching rate; PGD; Sexing.	
Categoria do assunto: 	L Ciência Animal e Produtos de Origem Animal	
Registro original: 	Embrapa Gado de Leite (CNPGL)	
Exemplares: 	CNPGL - (18004-1) - UPC - AP - DD -  -  -  - DIS