Data/hora: 25/06/2024 15:05:16

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 21/02/2014
Data da última atualização: | 05/02/2024
Autoria: | POLISSENI, J.; GRÁZIB, J. G. V. de; GUERRA, M. DE O.; MILEN, L. C.; CAMARGO, L. S. de A.; PETERS, V. M.
Afiliação: | JULIANA POLISSENI, UFJF; JOÃO GABRIEL VIANA DE GRÁZIB, UFJF; MARTHA DE OLIVEIRA GUERRA, UFJF; LARISSA CABRAL MILEN, UFJF; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; VERA MARIA PETERS, UFJF.
Título: | Impact of the use of gonadotropins on embryonic development - a rat model.
Ano de publicação: | 2013
Fonte/Imprenta: | JBRA Assisted Reproduction, v. 17, n. 1, p. 32-36, 2013.
Idioma: | Inglês
Conteúdo: | Objective: To evaluate the impact of in vivo superovulation on early embryonic development in a rat model. Methods: Experiment 1: Female Wistar rats were superovulated with injection of PMSG followed by hCG 48 hours later. Embryos were collected 24 h after hCG administration. Experiment 2: Female Wistar rats (n=16) were distrbuted to a control or superovulated group. Embryos were collected 48 and 72 h after hCG administration. The total number of embryonic structures and the embryo quality grades were analyzed by Chi-squared test. Between-group differences in ovarian weight were assessed by Student’s t-test. Results: Experiment 1: 931 embryos were collected from 66 animals, with a mean of 16.3±9.5 structures per animal. The superovulation was successful in 89.4% of the animals, and 98.2% of the embryos collected from superovulated animals were viable. Experiment 2: The superovulated group had heavier ovaries and produced more embryos than the control group (P < 0.001); however, these embryos contained fewer cells than controls at 72 hours after hCG administration (P<0.001). A greater percentage of embryos from the superovulated group were classified as grade I or grade II relative to embryos from the control group (P < 0.001 and P = 0.008, respectively). Conclusion: Superovulation was successful in the Wistar rat model and resulted in a greater number of embryos collected per animal. However, embryos produced as a result of superovulation developed more slowly than control embryos.
Palavras-Chave: | Animal model; Embryo development; Embryo grade.
Thesaurus Nal: | superovulation.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (21117-1) - UPC - AP - DD -  -  -  - DIS