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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/10/2012 |
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Data da última atualização: |
31/10/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CALEGARIO, R. F.; LABATE, M. T. V.; PERONI, LUÍS A.; STACH-MACHADO, D. R.; ASTUA, J. de F.; LABATE, C. A.; MACHADO, M. A.; KITAJIMA, E. W. |
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Afiliação: |
RENATA FAIER CALEGARIO, ESALQ; MÔNICA TERESA VENEZIANO LABATE, ESALQ; LUÍS ANTÔNIO PERONI, UNICAMP; DAGMAR RUTH STACH-MACHADO, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; CARLOS ALBERTO LABATE, ESALQ; MARCOS ANTÔNIO MACHADO, Centro APTA Citros Sylvio Moreira; ELLIOT WATANABE KITAJIMA, ESALQ. |
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Título: |
In vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C). |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Tropical Plant Pathology, n. 2, v. 37, p. 255-265, March-April, 2012. |
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ISSN: |
1982-5676 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter. Key words: Brevipalpus phoenicis, Cilevirus, Citrus sinensis, polyclonal antibody, serology. |
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Palavras-Chave: |
Polyclonal antibody. |
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Thesagro: |
Brevipalpus Phoenicis; Citrus Sinensis. |
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Thesaurus Nal: |
Cilevirus; Citrus; Citrus leprosis virus C; serology. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68741/1/in-vitro-expression.pdf
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Marc: |
LEADER 02278naa a2200301 a 4500
001 1937928
005 2012-10-31
008 2012 bl uuuu u00u1 u #d
022 $a1982-5676
100 1 $aCALEGARIO, R. F.
245 $aIn vitro expression and antiserum production against the movement protein of Citrus leprosis virus C (CiLV-C).
260 $c2012
520 $aCitrus leprosis, caused by Citrus leprosis virus C (CiLV-C), is currently considered the most important viral disease in the Brazilian citrus industry due to the high costs required for the chemical control of its vector, the mite Brevipalpus phoenicis. The pathogen induces a non-systemic infection and the disease is characterized by the appearance of localized lesions on citrus leaves, stems and fruits, premature fruit and leaf drop and dieback of stems. Attempts were made to promote in vitro expression of the putative cell-to-cell movement protein of CiLV-C in Escherichia coli and to produce a specific polyclonal antibody against this protein as a tool to investigate the virus-plantvector relationship. The antibody reacted strongly with the homologous protein expressed in vitro by ELISA, but poorly with the native protein present in leaf lesion extracts from sweet orange caused by CiLV-C. Reactions from old lesions were more intense than those from young lesions. Western blot and in situ immunolocalization assays failed to detect the native protein. These results suggest low expression of the movement protein (MP) in host tissues. Moreover, it is possible that the conformation of the protein expressed in vitro and used to produce the antibody differs from that of the native MP, hindering a full recognition of the latter. Key words: Brevipalpus phoenicis, Cilevirus, Citrus sinensis, polyclonal antibody, serology.
650 $aCilevirus
650 $aCitrus
650 $aCitrus leprosis virus C
650 $aserology
650 $aBrevipalpus Phoenicis
650 $aCitrus Sinensis
653 $aPolyclonal antibody
700 1 $aLABATE, M. T. V.
700 1 $aPERONI, LUÍS A.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aASTUA, J. de F.
700 1 $aLABATE, C. A.
700 1 $aMACHADO, M. A.
700 1 $aKITAJIMA, E. W.
773 $tTropical Plant Pathology$gn. 2
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
10/01/2018 |
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Data da última atualização: |
10/01/2018 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
AQUINO, S.; MARRACCINI, P.; MARIAC, C.; ANDRADE, A. C.; CROUZILAT, D.; KIWUKA, C.; ANTEN, N.; KOCHKO, A.; PONCET, V. |
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Afiliação: |
Sinara Aquino; Pierre Marraccini; Cédric Mariac; ALAN CARVALHO ANDRADE, SAPC; Dominique Crouzilat; Catherine Kiwuka; Niels Anten; Alexandre Kochko; Valérie Poncet. |
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Título: |
Targeting DREB subfamily genes AS candidates genes for drought tolerance polyformism in natural population of Coffea canephora. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: EUCARPIA GENETIC RESOURCES, 2017, Montpellier, France. Abstracts book... Montpellier, France: INRA, 2017. |
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Idioma: |
Inglês |
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Conteúdo: |
Coffea canephora (Robusta) provides 33% worldwide coffee production, 80% and 22% of Ugandan and Brazilian coffee production, respectively, Abiotic stress such as temperature variations of drought periods, aggravated bu climate changes, are factors that affect this production. |
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Palavras-Chave: |
Genetic diversity; Genetic resource; SNPs (single nucleotide polymorphism). |
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Thesagro: |
Coffea Canephora. |
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Thesaurus NAL: |
Abiotic stress; Climate change. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/170788/1/Targeting-DRE-subfamily.pdf
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Marc: |
LEADER 01176nam a2200277 a 4500
001 2084947
005 2018-01-10
008 2017 bl uuuu u00u1 u #d
100 1 $aAQUINO, S.
245 $aTargeting DREB subfamily genes AS candidates genes for drought tolerance polyformism in natural population of Coffea canephora.$h[electronic resource]
260 $aIn: EUCARPIA GENETIC RESOURCES, 2017, Montpellier, France. Abstracts book... Montpellier, France: INRA$c2017
520 $aCoffea canephora (Robusta) provides 33% worldwide coffee production, 80% and 22% of Ugandan and Brazilian coffee production, respectively, Abiotic stress such as temperature variations of drought periods, aggravated bu climate changes, are factors that affect this production.
650 $aAbiotic stress
650 $aClimate change
650 $aCoffea Canephora
653 $aGenetic diversity
653 $aGenetic resource
653 $aSNPs (single nucleotide polymorphism)
700 1 $aMARRACCINI, P.
700 1 $aMARIAC, C.
700 1 $aANDRADE, A. C.
700 1 $aCROUZILAT, D.
700 1 $aKIWUKA, C.
700 1 $aANTEN, N.
700 1 $aKOCHKO, A.
700 1 $aPONCET, V.
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Registro original: |
Embrapa Café (CNPCa) |
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