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Registro Completo |
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
28/09/2020 |
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Data da última atualização: |
07/12/2020 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
CARNEIRO, M. T.; PEREZ, D. V.; FEITOSA, R. C.; WASSERMAN, J. C. |
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Afiliação: |
MARCOS TAVARES CARNEIRO, UFF/FIOCRUZ; DANIEL VIDAL PEREZ, CNPS; RENATO CASTIGLIA FEITOSA, FIOCRUZ; JULIO CESAR WASSERMAN, UFF. |
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Título: |
Separation of Escherichia coli from natural samples for identification of sources and microcosm inoculation. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal Of Microbiology, v. 51, p. 2015-2020, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00374-2 |
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Idioma: |
Inglês |
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Conteúdo: |
Obtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and 1.10% of Escherichia coli were recovered from original samples. Although enduring unfavorable temperatures, salinities, and nutritional conditions, the inoculated microcosm populations grew approximately 310 times after 24 h. The final leachate contained cultivable cells in appropriate physiological states and quantities for inoculum in microcosm sets. The bacteria obtained from the leachate were also appropriate for surveys of microbial source tracking, because, in the developed procedure, organisms were separated from contaminants, while cell concentrations were sufficient for inocula. |
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Thesagro: |
Águas Residuais; Bactéria; Escherichia Coli; Rastreamento. |
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Thesaurus Nal: |
Strains; Wastewater. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218815/1/Separation-of-Escherichia-coli-from-natural-samples-2020.pdf
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Marc: |
LEADER 02106naa a2200241 a 4500
001 2125143
005 2020-12-07
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00374-2$2DOI
100 1 $aCARNEIRO, M. T.
245 $aSeparation of Escherichia coli from natural samples for identification of sources and microcosm inoculation.$h[electronic resource]
260 $c2020
520 $aObtaining uncultured Escherichia coli from natural waters is an important step in the study of microbes in the environment, which are critical for bacterial decay and microbial source tracking. The quality of the samples used can influence the assays, because high contaminant concentrations, differing cell ages, and physiologic states can impair results. The proposed separation is based on a three-step filtration method applied to replicates of seven samples from a sewage plant affluent, collected in different periods. Aliquots of the leachate were inoculated into microcosms, aiming to observe the cultivability of the cells. The assay resulted in colimetry values ranging between 104 and 105 cells. In the leachate, averages of 1.05% of total coliforms and 1.10% of Escherichia coli were recovered from original samples. Although enduring unfavorable temperatures, salinities, and nutritional conditions, the inoculated microcosm populations grew approximately 310 times after 24 h. The final leachate contained cultivable cells in appropriate physiological states and quantities for inoculum in microcosm sets. The bacteria obtained from the leachate were also appropriate for surveys of microbial source tracking, because, in the developed procedure, organisms were separated from contaminants, while cell concentrations were sufficient for inocula.
650 $aStrains
650 $aWastewater
650 $aÁguas Residuais
650 $aBactéria
650 $aEscherichia Coli
650 $aRastreamento
700 1 $aPEREZ, D. V.
700 1 $aFEITOSA, R. C.
700 1 $aWASSERMAN, J. C.
773 $tBrazilian Journal Of Microbiology$gv. 51, p. 2015-2020, 2020.
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Registro original: |
Embrapa Solos (CNPS) |
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| Registros recuperados : 299 | |
| 122. |  | TEIXEIRA, S. T.; LEAL, I.; SILVA, L. M. da; PEREZ, D. V.; WADT, P. G. S. Reciclagem de lodo de curtume em solos agrícolas. In: SEMINÁRIO ANUAL DE COOPERAÇÃO UFAC/UF., 2009, Rio Branco, AC. Parcerias em pesquisa e ação para a conservação e desenvolvimento sustentável: anais. Rio Branco, AC: UFAC, 2009. 4 p. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 123. | | PEREZ, D. V.; CAMPOS, D. V. B. de; TEIXEIRA, P. C. Ponto de carga zero (PCZ). In: TEIXEIRA, P. C.; DONAGEMMA, G. K.; FONTANA, A.; TEIXEIRA, W. G. (Ed.). Manual de métodos de análise de solo. 3. ed. rev. e ampl. Brasília, DF: Embrapa, 2017. pt. 2, cap. 9, p. 249-254.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
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| 126. | | FRANCO, M. G.; CORREA, S. M.; MARQUES, M.; PEREZ, D. V. Emission of volatile organic compounds and greenhouse gases from the aerobic bioremediation of soils contaminated with diesel. Water, Air, & Soil Pollution, v. 226, n. 3, 50, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 127. | | FRANCO, M. G.; CORREA, S. M.; MARQUES, M.; PEREZ, D. V. Emission of volatile organic compounds and greenhouse gases from the anaerobic bioremediation of soils contaminated with diesel. Water, Air, & Soil Pollution, v. 225, n. 2, 1879, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 128. | | SILVA, M. F. da; ANJOS, L. H. C. dos; PEREZ, D. V.; PEREIRA, M. G. Levantamento dos níveis de acidez e de alguns macronutrientes a partir da análise química de solos do Estado do Rio de Janeiro. In: CONGRESSO LATINO AMERICANO DE CIÊNCIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 1.; SIMPÓSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 4.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 6.; REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 11., 1996, Águas de Lindóia. Solo suelo 96. Campinas: Sociedade Brasileira de Ciência do Solo, 1996. 1 CD-ROM.| Biblioteca(s): Embrapa Solos. |
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| 129. | | CAMPOS, D. V. B. de; TEIXEIRA, P. C.; PEREZ, D. V.; SALDANHA, M. F. C. Hidrogênio extraível. In: TEIXEIRA, P. C.; DONAGEMMA, G. K.; FONTANA, A.; TEIXEIRA, W. G. (Ed.). Manual de métodos de análise de solo. 3. ed. rev. e ampl. Brasília, DF: Embrapa, 2017. pt. 2, cap. 5, p. 238-239.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Solos. |
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| Registros recuperados : 299 | |
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