|
|
Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
05/05/2004 |
Data da última atualização: |
05/06/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
CRUZ, J. C.; PEREIRA FILHO, I. A.; PEREIRA, F. T. F.; ALVARENGA, R. C. |
Afiliação: |
JOSE CARLOS CRUZ, CNPMS; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; RAMON COSTA ALVARENGA, CNPMS. |
Título: |
Avaliação de variedades de milho em diferentes densidades de plantio em sistema orgânico de produção. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROECOLOGIA, 1.; SEMINÁRIO INTERNACIONAL SOBRE AGROECOLOGIA, 4.; SEMINÁRIO ESTADUAL SOBE AGROECOLOGIA, 5., 2003, Porto Alegre. Conquistando a soberania alimentar: anais... Pelotas: Embrapa Clima Temperado; Emater-RS, 2003. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Thesagro: |
Agricultura Orgânica; Análise Foliar; Zea Mays. |
Thesaurus Nal: |
Varieties. |
Categoria do assunto: |
A Sistemas de Cultivo |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/53008/1/Avaliacao-variedades.pdf
|
Marc: |
LEADER 00816nam a2200193 a 4500 001 1484406 005 2018-06-05 008 2003 bl uuuu u00u1 u #d 100 1 $aCRUZ, J. C. 245 $aAvaliação de variedades de milho em diferentes densidades de plantio em sistema orgânico de produção.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE AGROECOLOGIA, 1.; SEMINÁRIO INTERNACIONAL SOBRE AGROECOLOGIA, 4.; SEMINÁRIO ESTADUAL SOBE AGROECOLOGIA, 5., 2003, Porto Alegre. Conquistando a soberania alimentar: anais... Pelotas: Embrapa Clima Temperado; Emater-RS$c2003 300 $c1 CD-ROM. 650 $aVarieties 650 $aAgricultura Orgânica 650 $aAnálise Foliar 650 $aZea Mays 700 1 $aPEREIRA FILHO, I. A. 700 1 $aPEREIRA, F. T. F. 700 1 $aALVARENGA, R. C.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
Data corrente: |
02/10/2015 |
Data da última atualização: |
13/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
Páginas: |
16 p. |
DOI: |
10.1371/journal.pone.0139051 |
Idioma: |
Inglês |
Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
Palavras-Chave: |
Drought stress; Soybean. |
Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
Thesaurus NAL: |
Drought tolerance; Soybeans; Transcriptome. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
|
Marc: |
LEADER 02592naa a2200361 a 4500 001 2079599 005 2017-11-13 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1371/journal.pone.0139051$2DOI 100 1 $aMARCOLINO-GOMES, J. 245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource] 260 $c2015 300 $a16 p. 520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. 650 $aDrought tolerance 650 $aSoybeans 650 $aTranscriptome 650 $aEstiagem 650 $aResistência à seca 650 $aSeca 650 $aSoja 653 $aDrought stress 653 $aSoybean 700 1 $aRODRIGUES, F. A. 700 1 $aFUGANTI-PAGLIARINI, R. 700 1 $aNAKAYAMA, T. J. 700 1 $aREIS, R. R. 700 1 $aFARIAS, J. R. B. 700 1 $aHARMON, F. G. 700 1 $aMOLINARI, H. B. C. 700 1 $aMOLINARI, M. D. C. 700 1 $aNEPOMUCENO, A. L. 773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Soja (CNPSO) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|