Data/hora: 27/05/2024 03:43:59

Biblioteca(s): 	Embrapa Arroz e Feijão.	
Data corrente: 	26/09/2017	
Data da última atualização: 	14/12/2017	
Autoria: 	PEREIRA, W. J.; BASSINELLO, P. Z.; BRONDANI, C.; VIANELLO, R. P.	
Afiliação: 	WENDELL JACINTO PEREIRA, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.	
Título: 	An improved method for RNA extraction from common bean seeds and validation of reference genes for qPCR.	
Ano de publicação: 	2017	
Fonte/Imprenta: 	Crop Breeding and Applied Biotechnology, Viçosa, MG, v. 17, n. 2, p. 150-158, abr./jun. 2017.	
ISSN: 	1518-7853	
DOI: 	10.1590/1984-70332017v17n2a22	
Idioma: 	Inglês	
Conteúdo: 	An RNA extraction method with high integrity and purity as well as the selection of adequate reference genes are prerequisites for gene expression analysis. For common bean seeds, there is no well-defined protocol that can be used in a laboratory routine for gene expression analysis. In this study, an extraction protocol for RNA from common bean seeds, which produced material with good integrity for qPCR (RIN &#8805; 6.5), was optimized. In addition, 10 reference genes were evaluated under qPCR standard conditions using different tissue samples of common beans. Gene stabilities were analyzed using the delta-CT method, Bestkeeper, NormFinder and geNorm approaches. The genes &#946;-tubulin and T197 were ranked as the most stable among the sample sets evaluated with different tissue samples, while PvAct and Pv18S were the least stable. To our knowledge, this is the first study evaluating RNA isolation methods and reference gene selection for seeds of Phaseolus vulgaris.	
Palavras-Chave: 	Gene normalization; Grain RNA extraction; Molecular breeding.	
Thesagro: 	Feijão; Melhoramento; Phaseolus vulgaris; RNA.	
Thesaurus Nal: 	Beans; Gene expression.	
Categoria do assunto: 	S Ciências Biológicas	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/164321/1/CNPAF-2017-cbab.pdf	
Registro original: 	Embrapa Arroz e Feijão (CNPAF)	
Exemplares: 	CNPAF - (34814-1) - UPC - AP - DD - 2017 -  - 2017 - DIS