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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
11/09/1997 |
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Data da última atualização: |
11/09/1997 |
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Autoria: |
SILVA, R. A. M. S.; RAMIREZ, L.; DAVILA, A. M. R.; VICTORIO, A. M.; PEREIRA, M. E. B. |
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Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS); UFMS. Centro Universitario de Corumba. Departamento de Ciencias do Ambiente (Corumba, MS). |
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Título: |
An investigation of in vitro phagocytosis of Trypanosoma evansi by rat peritoneal macrophages: increase mediated by parasite-specific antibodies. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Memorias do Instituto Oswaldo Cruz, Rio de Janeiro, v.91, p.105, 1996. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Trypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87% at 15 min, 100% at 30 min, and 94,26% at 45 min. We conclude that the absence of phagocytosis in the group A could be explained normal macrophages have some difficulty to uptake the parasites; although some of parasites attach to the Mo, they are not internalized as observed in experiments with T.brucei. The espace from phagocytosis is even improved because of short cell contacts due to permanent movement of the cells concerned. However, in the presence of parasite-specific antibodies (group B), internalization and degradationof the trypanosomes took place. So, according to Shakibaei & Frevert, in the absence of antibodies, bloodstream trypanosomes are protected against phagocytosis by the surface coat. In immune animals the parasites are removed from the bloodsteam by phagocytic cells. The opsonization with specific antibodies or proteolytic removal of the coat result in rapid uptake of the parasites by Mos. MenosTrypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87%... Mostrar Tudo |
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Thesaurus Nal: |
Trypanosoma evansi. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03040naa a2200181 a 4500
001 1791630
005 1997-09-11
008 1996 bl uuuu u00u1 u #d
100 1 $aSILVA, R. A. M. S.
245 $aAn investigation of in vitro phagocytosis of Trypanosoma evansi by rat peritoneal macrophages$bincrease mediated by parasite-specific antibodies.
260 $c1996
520 $aTrypanosoma evansi causes and usually fatal disease in rats, mice, horses and dogs. Phagocytosis and the intracellular killing of pathogens in probaly one of the most important defense system available to an animal. Macrophages (Mo) are central cells in the action and regulation of the immune network. This is in view of their function as phagocytic and antigen-presenting cells, are their release of mediators, expression of surface receptors, and interaction with T and B cells. This experiment was developed to evaluate the influence of parasitic-specific antibody in the phagocytosis by peritoneal Mo of rats. Peritoneal Mo, 1,45 x 10 6 were isolated from Wistar rats pretreated 3 days before single intraperitoneal dose of tioglicolate. The Mo were then precultivated for 24 h. on coverslips in culture flasks. Trypanosomes, 8,7 x 10 6(final concentration) were added to the adherent macrophages and were cocultivated at 37 oC for 5, 15, 30, 45 min. in EAGLE medium (Instituto Adolfo Lutz, SP). The trypanosome/ Mo proportion was 6:1. The phagocytosis test was performed in two groups: group A (Mo treated with normal serum) and B (Mo treated with 200 ul of serum from horse naturally infected by T.evansi). The parasites were not observed attached or phagocytosed by Mo in group A. In group B trypanosomes attached to 51,26% of the Mos within 5 min, 17,20% within 15 min, 46,15% within 30 min, 10,65% within 45 min. The phagocytosis process was observed in 96,63% of the Mos at 5 min, 83,87% at 15 min, 100% at 30 min, and 94,26% at 45 min. We conclude that the absence of phagocytosis in the group A could be explained normal macrophages have some difficulty to uptake the parasites; although some of parasites attach to the Mo, they are not internalized as observed in experiments with T.brucei. The espace from phagocytosis is even improved because of short cell contacts due to permanent movement of the cells concerned. However, in the presence of parasite-specific antibodies (group B), internalization and degradationof the trypanosomes took place. So, according to Shakibaei & Frevert, in the absence of antibodies, bloodstream trypanosomes are protected against phagocytosis by the surface coat. In immune animals the parasites are removed from the bloodsteam by phagocytic cells. The opsonization with specific antibodies or proteolytic removal of the coat result in rapid uptake of the parasites by Mos.
650 $aTrypanosoma evansi
700 1 $aRAMIREZ, L.
700 1 $aDAVILA, A. M. R.
700 1 $aVICTORIO, A. M.
700 1 $aPEREIRA, M. E. B.
773 $tMemorias do Instituto Oswaldo Cruz, Rio de Janeiro$gv.91, p.105, 1996. Suplemento.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
10/12/2015 |
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Data da última atualização: |
15/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
CEREIJO, C. R.; SANTANA, H.; BRUNALE, P. P. de M.; SIQUEIRA, F. G. de; BRASIL, B. dos S. A. F. |
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Afiliação: |
Carolina R. Cereijo; Hugo Santana; PATRICIA PORTELA DE M BRUNALE, CNPAE; FELIX GONCALVES DE SIQUEIRA, CNPAE; BRUNO DOS SANTOS ALVES FIGUEIREDO BRASIL, CNPAE. |
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Título: |
Seleção de microalgas com capacidade de crescimento no efluente da lagoa de estabilização de POME. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. |
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Páginas: |
p. 67-69 |
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Idioma: |
Português |
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Palavras-Chave: |
Microalgas; POME. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135373/1/resumo-22.pdf
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Marc: |
LEADER 00662nam a2200181 a 4500
001 2031391
005 2016-02-15
008 2015 bl uuuu u00u1 u #d
100 1 $aCEREIJO, C. R.
245 $aSeleção de microalgas com capacidade de crescimento no efluente da lagoa de estabilização de POME.$h[electronic resource]
260 $aIn: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia$c2015
300 $ap. 67-69
653 $aMicroalgas
653 $aPOME
700 1 $aSANTANA, H.
700 1 $aBRUNALE, P. P. de M.
700 1 $aSIQUEIRA, F. G. de
700 1 $aBRASIL, B. dos S. A. F.
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