| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite. |
|
Data corrente: |
14/12/2023 |
|
Data da última atualização: |
11/03/2024 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SILVA, J. R. da; SILVA, J. F. M.; PEREIRA, M. F.; TORRES, A. R.; GONÇALVES, M. S.; PRATA, M. C. de A.; BRITO, M. A. V. P. e; COSTA, G. M. da; RIBEIRO, J. B. |
|
Afiliação: |
JULIANA ROSA DA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; JOICE FÁTIMA MOREIRA SILVA, UNIVERSIDADE FEDERAL DE LAVRAS; MONALESSA FÁBIA PEREIRA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; ADALGISA RIBEIRO TORRES; MAYSA SERPA GONÇALVES, UNIVERSIDADE FEDERAL DE LAVRAS; MARCIA CRISTINA DE AZEVEDO PRATA, CNPGL; MARIA APARECIDA VASCONCELOS PAIVA E BRITO; GERALDO MÁRCIO DA COSTA, UNIVERSIDADE FEDERAL DE LAVRAS; JOAO BATISTA RIBEIRO, CNPGL. |
|
Título: |
Is Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis? |
|
Ano de publicação: |
2024 |
|
Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 55, n. 1, p. 889-900, 2024. |
|
DOI: |
https://doi.org/10.1007/s42770-023-01181-1 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Staphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations. MenosStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic tar... Mostrar Tudo |
|
Palavras-Chave: |
Infecção intramamária; Modelo de invertebrados; Virulência bacteriana. |
|
Thesagro: |
Bovino; Doença Animal. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/262967/1/Is-Galleria-mellonella-model-a-good-alternative-to-study-virulence.pdf
|
|
Marc: |
LEADER 02652naa a2200289 a 4500
001 2159710
005 2024-03-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-023-01181-1$2DOI
100 1 $aSILVA, J. R. da
245 $aIs Galleria mellonella model a good alternative to study virulence in Staphylococcus aureus from bovine mastitis?$h[electronic resource]
260 $c2024
520 $aStaphylococcus aureus is one of the agents of bovine mastitis of hardest control due to a complex pathogenesis comprising a variety of virulence factors, which ensures its persistence in the mammary gland, causing significant health and economic losses. Therefore, understanding the pathogenesis of this agent is imperative. Galleria mellonella has stood out as an invertebrate animal model for the study of infectious diseases that affect several hosts. This work aimed to evaluate G. mellonella larvae as an experimental model for the study of virulence phenotypes in an S. aureus population isolated from bovine mastitis. Thirty genetically divergent S. aureus strains were chosen based on PFGE analysis. After experimental infection, larvae survival rates, bacterial growth in hemolymph, melanization intensity of the dorsal vessel, and histological characteristics of the infected tissues were evaluated. The G. mellonella model showed a clear diversity in the S. aureus pathogenicity pattern, allowing the differentiation of strains with virulence phenotypes ranging from high to low degrees. Histological analysis confirmed that the strains tested were capable of inducing the formation of nodules and melanization spots in the dorsal vessels of the larvae in different magnitudes. The strains 16S-717, 19C-828, and 31S-1443 presented the highest virulence intensity among the bacteria tested and will be used further for the generation of S. aureus mutant populations to prospect genetic targets aimed to develop control strategies of bovine mastitis. Altogether, our results suggest that G. mellonella is an attractive and low-cost animal model for characterizing virulence phenotypes of large S. aureus populations.
650 $aBovino
650 $aDoença Animal
653 $aInfecção intramamária
653 $aModelo de invertebrados
653 $aVirulência bacteriana
700 1 $aSILVA, J. F. M.
700 1 $aPEREIRA, M. F.
700 1 $aTORRES, A. R.
700 1 $aGONÇALVES, M. S.
700 1 $aPRATA, M. C. de A.
700 1 $aBRITO, M. A. V. P. e
700 1 $aCOSTA, G. M. da
700 1 $aRIBEIRO, J. B.
773 $tBrazilian Journal of Microbiology$gv. 55, n. 1, p. 889-900, 2024.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Roraima. |
|
Data corrente: |
03/02/2021 |
|
Data da última atualização: |
18/06/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
PINEDO-PANDURO, M.; CHAGAS, E. A.; PAREDES-DAVILA, E.; ABANTO-RODRIGUEZ, C.; BARDALES-LOZANO, R.; CHAGAS, P. C.; MELO, V. F.; CASTILLO-TORRES, D. del; PANDURO- TENAZOA, N. M. |
|
Afiliação: |
EDVAN ALVES CHAGAS, CPAF-RR. |
|
Título: |
Individual selection in a genetic test with 43 advanced progenies of camu-camu Myrciaria dubia Kunth (McVaugh) in floodable plot near Iquitos, Peruvian Amazon. |
|
Ano de publicação: |
2020 |
|
Fonte/Imprenta: |
Scientia Agropecuaria, v. 11, n. 3, p. 345-355, 2020. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The research summarized and validated the individual selection of camu-camu (Myrciaria dubia (Kunth) McVaugh) plants. 43 progenies, previously selected in genetic tests and fields of producers of flood areas, were evaluated. Each progeny was installed in a row of 10 half-sister plants, with a space of 3x2 m. Using the SELEGEN (Reml / Blup) Model 63 program, four crops were analyzed, and individuals were selected for each of the 16 descriptors evaluated. For fruit yield, the Tipishca-Río Tigre population stood out, occupying 5 of the first 10 places. The individuals TT0725-4 (Tigre-Tipishca) turned out to be a natural recombinant for fruit yield / weight. And individual 64-8 turned out to be triple recombinant (yield / fruit weight / ascorbic acid). The descriptors with the highest genetic control with r ≥ 0.30 were: average fruit weight, brix degrees, plant height, number of basal branches and petiole length. The criteria of similarity of the morphological markers with the fruit yield are proposed as predictive indexes of productivity. The plant height at two years of age was the most important. |
|
Palavras-Chave: |
Amazon rainforest; Fruit food; Genetic evaluation. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220882/1/Genot-Peru-Scientia-Agropv11n3p345355-2020-02.16.04.031.00.03.pdf
|
|
Marc: |
LEADER 01951naa a2200253 a 4500
001 2129784
005 2021-06-18
008 2020 bl uuuu u00u1 u #d
100 1 $aPINEDO-PANDURO, M.
245 $aIndividual selection in a genetic test with 43 advanced progenies of camu-camu Myrciaria dubia Kunth (McVaugh) in floodable plot near Iquitos, Peruvian Amazon.$h[electronic resource]
260 $c2020
520 $aThe research summarized and validated the individual selection of camu-camu (Myrciaria dubia (Kunth) McVaugh) plants. 43 progenies, previously selected in genetic tests and fields of producers of flood areas, were evaluated. Each progeny was installed in a row of 10 half-sister plants, with a space of 3x2 m. Using the SELEGEN (Reml / Blup) Model 63 program, four crops were analyzed, and individuals were selected for each of the 16 descriptors evaluated. For fruit yield, the Tipishca-Río Tigre population stood out, occupying 5 of the first 10 places. The individuals TT0725-4 (Tigre-Tipishca) turned out to be a natural recombinant for fruit yield / weight. And individual 64-8 turned out to be triple recombinant (yield / fruit weight / ascorbic acid). The descriptors with the highest genetic control with r ≥ 0.30 were: average fruit weight, brix degrees, plant height, number of basal branches and petiole length. The criteria of similarity of the morphological markers with the fruit yield are proposed as predictive indexes of productivity. The plant height at two years of age was the most important.
653 $aAmazon rainforest
653 $aFruit food
653 $aGenetic evaluation
700 1 $aCHAGAS, E. A.
700 1 $aPAREDES-DAVILA, E.
700 1 $aABANTO-RODRIGUEZ, C.
700 1 $aBARDALES-LOZANO, R.
700 1 $aCHAGAS, P. C.
700 1 $aMELO, V. F.
700 1 $aCASTILLO-TORRES, D. del
700 1 $aPANDURO- TENAZOA, N. M.
773 $tScientia Agropecuaria$gv. 11, n. 3, p. 345-355, 2020.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Roraima (CPAF-RR) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
