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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
05/10/2011 |
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Data da última atualização: |
10/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, M. K. F. de; CARVALHO, K. de; SOUZA, F. S. de; MARUR, C. J.; PEREIRA, L. F. P.; BESPALHOK FILHO, J. C.; VIEIRA, L. G. E. |
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Afiliação: |
INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, SAPC; UNIVERSIDADE FEDERAL DO PARANÁ; UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Drought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Environmental and Experimental Botany, v.72, n. 2, p. 242-250, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
In this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress. MenosIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was m... Mostrar Tudo |
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Palavras-Chave: |
Citrus rootstock; Osmotic adjustment. |
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Thesaurus Nal: |
Drought tolerance; Oxidative stress; Proline. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/42905/1/Drought-tolerance-and-antioxidant.pdf
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Marc: |
LEADER 02726naa a2200253 a 4500
001 1902435
005 2024-01-10
008 2011 bl uuuu u00u1 u #d
100 1 $aCAMPOS, M. K. F. de
245 $aDrought tolerance and antioxidant enzymatic activity in transgenic 'Swingle' citrumelo plants over-accumulating proline.$h[electronic resource]
260 $c2011
520 $aIn this study we investigated the effects of the high endogenous proline level on water relations, gas exchange and antioxidant enzymatic activity in leaves of transgenic 'Swingle' citrumelo rootstocks transformed with the P5CSF129A gene coding for the key-enzyme for proline synthesis, under water deficit. Leaf total water, osmotic and pressure potentials, stomatal conductance, photosynthetic rates and xylem sapflowwere evaluated in non-transformed control and transgenic plants during water deficit treatment. Malondialdehyde (MDA) content, catalase (CAT; EC 1.11.1.6), superoxide dismutase (SOD; EC 1.15.1.1) and ascorbate peroxidase (APX; EC 1.11.1.11) activities were quantified in leaves collected based on their total water potential, representing the following conditions: irrigated (w = -1.3 MPa), moderate stress (w = -2.3 to - 2.5 MPa), severe stress (w = -3.8 to - 3.9 MPa) and recovery (24 h after re-irrigation: w = -1.3 to - 1.9 MPa). Osmotic adjustment was observed in transgenic plants until 11 days after withholding water, while pressure potential in non-transformed controls was close to zero after nine days of water deprivation. This superior maintenance of turgor pressure in leaves of transgenic plants led to higher stomatal conductance, photosynthetic and transpiration rates when compared to non-transgenic plants. Drought caused a significant decrease in APX and SOD activities in control plants, followed by an increase after re-watering. On the other hand, CAT was more active in control than in transgenic plants under irrigated condition and both stress levels. Our results suggest that transgenic plants were able to cope with water deficit better than non-transformed controls since the high endogenous proline level acted not only by mediating osmotic adjustment, but also by contributing to gas exchange parameters and ameliorating deleterious effects of drought-induced oxidative stress.
650 $aDrought tolerance
650 $aOxidative stress
650 $aProline
653 $aCitrus rootstock
653 $aOsmotic adjustment
700 1 $aCARVALHO, K. de
700 1 $aSOUZA, F. S. de
700 1 $aMARUR, C. J.
700 1 $aPEREIRA, L. F. P.
700 1 $aBESPALHOK FILHO, J. C.
700 1 $aVIEIRA, L. G. E.
773 $tEnvironmental and Experimental Botany$gv.72, n. 2, p. 242-250, 2011.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca |
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| Registros recuperados : 172 | |
| 101. |  | KITZBERGER, C. S. G.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; SERA, T.; SILVA, J. B. G. D.; BENASSI, M. de T. Diterpenes in green and roasted coffee of Coffea arabica cultivars growing in the same edapho-climatic conditions. Journal of Food Composition and Analysis, v. 30, n. 1, P. 52-57, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 102. | | SILVA, C. R. M. da; LOPES, F. R.; CARARETO, C. M. A.; VANZELA, A. L. L.; DOMINGUES, D. S.; PEREIRA, L. F. P. Distribuição de elementos transponíveis em cromossomos de coffea arabica. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 7., 2011, Araxá. Anais... Brasília, DF: Embrapa Café, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 103. | | GARRETT, R.; SCHMIDT, E. M.; PEREIRA, L. F. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; EBERLIN, M. N.; REZENDE, C. M. Discrimination of arabica coffee cultivars by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry and chemometrics. LWT - Food Science and Technology, v. 50, n. 2, p. 496-502, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 104. | | DOMINGUES, D. S.; REIS JUNIOR, O.; SANTOS, T. B. dos; SILVA, J. C.; LORENZETTI, A. P.; SOARES, J. D. M.; PEREIRA, L. F. P. Nitrogen-starvation responsive microRNAs in Coffea arabica roots: it's this a 3-player game? In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 11., 2015, Iguassu Falls, Brazil. Summaries... Rockville, USA: American Society of Plant Biologists, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 105. | | CARVALHO, K. de; BESPALHOK FILHO, J. C.; SANTOS, T. B. dos; SOUZA, S. G. H. de; VIEIRA, L. G. E.; PEREIRA, L. F. P.; DOMINGUES, D. S. Nitrogen starvation, salt and heat stress in coffee (coffea arabica L.): identification and validation of new genes for qPCR normalization. Molecular Biotechnology, v. 53, n. 3, p. 315-325, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 106. | | PEREIRA, L. F. P.; DIOLA, V.; CAÇÃO, S. M. B.; CAIXETA, E. T.; BRITO, G. G. de; ZAMBOLIN, E. M.; LOUREIRO, M. E. Mapeamento integrado de Coffea arabica: identificação de marcadores e clones BAC para o gene de resistência a ferrugem. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 23. 2010, Bali, Indonesia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 107. | | GEROMEL, C.; PEREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Metabolismo de açúcares durante o desenvolvimento de frutos de café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 4., 2005, Londrina. Anais... Brasília, DF: Embrapa Café, 2005.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 108. | | MOLINARI, H. B. C.; MARUR, C. J.; BESPALHOK FILHO, J. C.; KOBAYASHI, A. K.; PILEGGI, M.; LEITE JÚNIOR, R. P.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Osmotic adjustment in transgenic citrus rootstock Carrizo citrange (Citrus sinensis Osb. x Poncirus trifoliata L. Raf.) overproducing proline. Plant Science, v. 167, n. 6, p. 1375-1381, 2004.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Café. |
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| 109. | | DIOLA, V.; CAÇÃO, S. M. B.; CAIXETA, E. T.; BRITO, G. G.; ZAMBOLIM, E. M.; PEREIRA, L. F. P.; LOUREIRO, M. E. Genetic and physical mapping of the resistant gene for H. vastatrix race II in Coffea arabica. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 23. 2010, Bali, Indonesia.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 110. | | IVAMOTO, S. T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; ANDRADE, D. S.; ALVES, L. C.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. In silico analysis of cytochrome p450 genes involved in the metabolism of diterpenes in Coffea. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos...| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 111. | | IVAMOTO, S. T.; CALEDÓN, J. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; BOHLMANN, J.; DOMINGUES, D. S.; PEREIRA, L. F. P. Transcriptome analysis and characterization of genes involved in diterpene biosynthesis in coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 105| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 112. | | YUYAMA, P. M.; REIS JUNIOR, O.; IVAMOTO, S. T.; DOMINGUES, D. S.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; CHARMETANT, P.; LEROY, T.; PEREIRA, L. F. P. Transcriptome analysis in Coffea eugenioides, an Arabica coffee ancestor, reveals differentially expressed genes in leaves and fruits. Molecular Genetics and Genomics, v. 291, n. 1, p. 323-336. 2016| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
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| 114. | | GEROMEL, C.; FERREIRA, L. P.; BOTTCHER, A.; POT, D.; PEREIRA, L. F. P.; LEROY, T.; VIEIRA, L. G. E.; MAZZAFERA, P.; MARRACCINI, P. Sucrose metabolism during fruit development in coffea racemosa. Annals of Applied Biology, v. 152, n. 2, p.179-187, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Café. |
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| 115. | | GEROMEL, C.; FERREIRA, L. P.; CAVALARI, A. A.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; LEROY, T.; MAZZAFERA, P.; MARRACCINI, P. Sugar metabolism during coffee fruit development. In:INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 20., 2004, Bangalore, India. Anais... Montpellier, France: Association for Science and Information on Coffee, 2005. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 116. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINIECK, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using a Coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping approaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 117. | | PEREIRA, L. F. P.; CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINECKY, F.; CAIXETA, E. T.; ANDRADE, A. C.; VIEIRA, L. G. E. Using a coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping approaches. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, p. 276.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 118. | | CAÇÃO, S. M. B.; SILVA, N. V.; ALVES, G. S. C.; VINECKY, F.; DINIZ, L. E. C.; ANDRADE, A. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Using coffea arabica bacterial artificial chromosome library for gene cloning and integrative mapping aproaches. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas, Brazil. Programme abstracts. Montpellier, France: Association for Science and Information on Coffee, 2008. PB633.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 119. | | SCHOLZ, M. B. S.; PAGIATTO, N. F.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; CHARMETANTE, P.; LEROYE, T. Validation of near-infrared spectroscopy for the quantification of cafestol and kahweol in green coffee. Food Research International, v. 61, p. 176-182, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 120. | | LANNES, S. D.; BOUCHET, S.; FERREIRA, L. P.; LEROY, T.; IVAMOTO, S. T.; MARRACINI, P.; PEREIRA, L. F. P.; VIEIRA, L. G.; POT, D. Polimorfismos nucleotídicos de genes envolvidos nas características químicas do grão de café. Complementaridade das estratégias in silico e in vivo. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 172 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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