|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Café. |
Data corrente: |
19/09/2017 |
Data da última atualização: |
03/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
KOBAYASHI, A. K.; VIEIRA, L. G. E.; BESPALHOK FILHO, J. C.; LEITE JUNIOR, R. P.; PEREIRA, L. F. P.; MOLINARI, H. B. C.; MARQUES, V. V. |
Afiliação: |
ADILSON KENJI KOBAYASHI, CNPAE; LUIZ GONZAGA E. VIEIRA; JOÃO CARLOS BESPALHOK FILHO; RUI PEREIRA LEITE JUNIOR; LUIZ FILIPE P. PEREIRA; HUGO BRUNO CORREA MOLINARI, CNPAE; VIVIANI V. MARQUES. |
Título: |
Enhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
European Journal of Plant Pathology, v. 149, n. 4, p. 865-873, 2017. |
DOI: |
10.1007/s10658-017-1234-5 |
Idioma: |
Inglês |
Conteúdo: |
Citrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker. MenosCitrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulati... Mostrar Tudo |
Palavras-Chave: |
Bacterial disease resistance; Citrus canker; Mature tissue transformation. |
Thesagro: |
Agrobacterium Tumefaciens. |
Thesaurus Nal: |
Agrobacterium radiobacter; Antimicrobial peptides. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02638naa a2200277 a 4500 001 2075843 005 2024-01-03 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s10658-017-1234-5$2DOI 100 1 $aKOBAYASHI, A. K. 245 $aEnhanced resistance to citrus canker in transgenic sweet orange expressing the sarcotoxin IA gene.$h[electronic resource] 260 $c2017 520 $aCitrus canker, caused by the bacterial pathogen Xanthomonas citri subp. Citri (Xcc), is a serious disease reported in most citrus-producing areas around the world. Although different levels of field resistance to citrus canker have been reported in sweet oranges, they are usually not sufficient to provide adequate control of the disease. Ectopic over-expression of antibacterial genes is one of the potential strategies to increase plant resistance to bacterial diseases. Previous in vitro results showed that sarcotoxin IA, an antimicrobial peptide isolated from the flesh fly (Sarcophaga peregrina), can be efficient to control different plant pathogenic bacteria, including Xcc. Transgenic BPera^ sweet orange (Citrus sinensis [L.] Osbeck) plants constitutively expressing the sarcotoxin IA peptide fused to the PR1a signal peptide from Nicotiana tabacum for secretion in the intercellular space were obtained by Agrobacteriummediated transformation using thin sections of mature explants. Citrus canker resistance evaluation in leaves of transgenic and non-transgenic plants was performed through inoculations with Xcc by infiltration and spraying. The Xcc population was up to 2 log unit lower in leaves of the transgenic plants compared to those of non-transgenic controls. Incidence of canker lesions was significantly higher in non-transformed controls (>10 lesions/cm2) than in the transgenic plants (<5 lesions/ cm2) after injection infiltration or spraying with Xcc inoculum. Accumulation of sarcotoxin IA peptide in sweet orange tissue did not cause any deleterious effects on the growth and development of the transgenic plants, indicating this approach is suitable to provide resistance to citrus canker. 650 $aAgrobacterium radiobacter 650 $aAntimicrobial peptides 650 $aAgrobacterium Tumefaciens 653 $aBacterial disease resistance 653 $aCitrus canker 653 $aMature tissue transformation 700 1 $aVIEIRA, L. G. E. 700 1 $aBESPALHOK FILHO, J. C. 700 1 $aLEITE JUNIOR, R. P. 700 1 $aPEREIRA, L. F. P. 700 1 $aMOLINARI, H. B. C. 700 1 $aMARQUES, V. V. 773 $tEuropean Journal of Plant Pathology$gv. 149, n. 4, p. 865-873, 2017.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Agroenergia (CNPAE) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
25/11/1993 |
Data da última atualização: |
10/07/2024 |
Autoria: |
SOUZA, H. B. de. |
Afiliação: |
HILKIAS BERNARDO DE SOUZA, INSTITUTO AGRONOMICO DO NORTE. |
Título: |
O cipó babão (Cissus gongylodes Baker) um agente coagulante do latex de Hevea. |
Ano de publicação: |
1956 |
Fonte/Imprenta: |
Boletim Técnico. IAN, Belém, PA, n. 31, p. 163-186, jun. 1956. |
Idioma: |
Português |
Palavras-Chave: |
Analysis; Cipó babão; Cissus gongylodes. |
Thesagro: |
Análise; Borracha; Coagulação; Hevea; Latex. |
Thesaurus NAL: |
coagulation; rubber. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/69991/1/IAN-BT31-P163.pdf
|
Marc: |
LEADER 00615naa a2200229 a 4500 001 1375650 005 2024-07-10 008 1956 bl uuuu u00u1 u #d 100 1 $aSOUZA, H. B. de 245 $aO cipó babão (Cissus gongylodes Baker) um agente coagulante do latex de Hevea. 260 $c1956 650 $acoagulation 650 $arubber 650 $aAnálise 650 $aBorracha 650 $aCoagulação 650 $aHevea 650 $aLatex 653 $aAnalysis 653 $aCipó babão 653 $aCissus gongylodes 773 $tBoletim Técnico. IAN, Belém, PA$gn. 31, p. 163-186, jun. 1956.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Amazônia Oriental (CPATU) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Nenhum registro encontrado para a expressão de busca informada. |
|
|