Data/hora: 22/06/2024 07:09:26

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Mandioca e Fruticultura.
Data corrente: | 30/10/2001
Data da última atualização: | 28/12/2005
Autoria: | BESPALHOK FILHO, J. C.; KOBAYASHI, A. K.; PEREIRA, L. F. P.; HISSANO, Z.; VIEIRA, L. G. E.
Afiliação: | UEL; IAPAR.
Título: | In vitro adventitious shoot regeneration from sweet orange using thin epicotyl sections.
Ano de publicação: | 2001
Fonte/Imprenta: | Crop Breeding and Applied Biotechnology, Londrina, v.1, n.1, p.27-34, 2001.
ISSN: | 1518-7853
Idioma: | Inglês
Conteúdo: | A system for in vitro plant regeneration from thin transversal epicotyl sections of sweet orange (Citrus sinensis L. Osbeck cvs. Pera, Valencia and Folha Murcha) was developed. Thin section explants (1-2 mm) from seedlings produced adventitious shoot buds on MS medium supplemented with BA (0.5-5 µM). Optimal shoot induction was achieved on 2-5 µM BA. Explants with buds were transferred to elongation media in order to improve the recovery of normal shoots. Higher number of elongated shoots was obtained on medium with 0.5 µM BA. Elongated shoots were rooted on half strength MS medium without growth regulators and transferred to soil for acclimatization. The advantages of this system for transformation are discussed.
Palavras-Chave: | Adventitious buds; Epicotyl; Orange; Regeneration; Shoot multiplication.
Thesagro: | Citrus Sinensis; Laranja; Regeneração.
Categoria do assunto: | --
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)

Exemplares:

CNPAF - (17772-1) - ADD - AP - -- - 631.05 - C - 2001.00004 - DIS
CNPMF - (22511-1) - UPC - AP - -- -  -  -  - DIS