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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/04/2004 |
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Data da última atualização: |
23/04/2004 |
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Autoria: |
PEREIRA, L. F.; ERICKSON, L. |
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Título: |
An investigation of phenylalanine ammonia lyase and peroxidase during post-harvest deterioration of cassava roots. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Salvador, v.17, p.88, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objective of this study was to exanime mRNA transcription and enzyme activity of phenylalanine ammonia lyase (PAL) and peroxidase in cassava during root post-harvest deterioration. Cassava roots were transversely cut into 3.5-4.0 cm thick slices, and incubated in the dark on moistened paper towels in covered plastic containers. From the cut root slices, two sub-sections were collected for analysis, the outermost laver of 0.5 cm, and an inner layer 0.5 cm thick, located 1 cm in from the outermost layer. The inner layers showed a gradual increase in PAL activity. In contrast, the increase in PAL activity in the outer layers was more rapid, reaching a maximum in the initial hours after injury. Transcripts were detected as early as 6 h post injury, and increased rapidly to a maximum at 12 h; however, by 24 h PAL transcripts were scarcely detectable and increased gradually thereafter up to the experiment. Total peroxidase activity started to increase 24 hors after wounding in both layers. However, the inner layers showed an increase in both soluble and ionically bound-peroxidases, whereas the outer layers showed a higher level of the ionically-bound form in comparison to the soluble form. When AIP, an inhibitor of PAL, was applied to the roots, there were no visible differences in the pattern of root deterioration. However, there were a decrease in both PAL transcription and enzyme activity. Furthermore, differences in the balance of peroxidases was also observed in roots treated with AIP. MenosAbstract: The objective of this study was to exanime mRNA transcription and enzyme activity of phenylalanine ammonia lyase (PAL) and peroxidase in cassava during root post-harvest deterioration. Cassava roots were transversely cut into 3.5-4.0 cm thick slices, and incubated in the dark on moistened paper towels in covered plastic containers. From the cut root slices, two sub-sections were collected for analysis, the outermost laver of 0.5 cm, and an inner layer 0.5 cm thick, located 1 cm in from the outermost layer. The inner layers showed a gradual increase in PAL activity. In contrast, the increase in PAL activity in the outer layers was more rapid, reaching a maximum in the initial hours after injury. Transcripts were detected as early as 6 h post injury, and increased rapidly to a maximum at 12 h; however, by 24 h PAL transcripts were scarcely detectable and increased gradually thereafter up to the experiment. Total peroxidase activity started to increase 24 hors after wounding in both layers. However, the inner layers showed an increase in both soluble and ionically bound-peroxidases, whereas the outer layers showed a higher level of the ionically-bound form in comparison to the soluble form. When AIP, an inhibitor of PAL, was applied to the roots, there were no visible differences in the pattern of root deterioration. However, there were a decrease in both PAL transcription and enzyme activity. Furthermore, differences in the balance of peroxidases was also observed i... Mostrar Tudo |
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Categoria do assunto: |
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Marc: |
LEADER 01969naa a2200133 a 4500
001 1651989
005 2004-04-23
008 1998 bl uuuu u00u1 u #d
100 1 $aPEREIRA, L. F.
245 $aAn investigation of phenylalanine ammonia lyase and peroxidase during post-harvest deterioration of cassava roots.
260 $c1998
520 $aAbstract: The objective of this study was to exanime mRNA transcription and enzyme activity of phenylalanine ammonia lyase (PAL) and peroxidase in cassava during root post-harvest deterioration. Cassava roots were transversely cut into 3.5-4.0 cm thick slices, and incubated in the dark on moistened paper towels in covered plastic containers. From the cut root slices, two sub-sections were collected for analysis, the outermost laver of 0.5 cm, and an inner layer 0.5 cm thick, located 1 cm in from the outermost layer. The inner layers showed a gradual increase in PAL activity. In contrast, the increase in PAL activity in the outer layers was more rapid, reaching a maximum in the initial hours after injury. Transcripts were detected as early as 6 h post injury, and increased rapidly to a maximum at 12 h; however, by 24 h PAL transcripts were scarcely detectable and increased gradually thereafter up to the experiment. Total peroxidase activity started to increase 24 hors after wounding in both layers. However, the inner layers showed an increase in both soluble and ionically bound-peroxidases, whereas the outer layers showed a higher level of the ionically-bound form in comparison to the soluble form. When AIP, an inhibitor of PAL, was applied to the roots, there were no visible differences in the pattern of root deterioration. However, there were a decrease in both PAL transcription and enzyme activity. Furthermore, differences in the balance of peroxidases was also observed in roots treated with AIP.
700 1 $aERICKSON, L.
773 $tRevista Brasileira de Mandioca, Salvador$gv.17, p.88, nov., 1998. Suplemento.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Registros recuperados : 180 | |
| 4. |  | CAÇÃO, S. M. B.; SILVA, N. V. e; PEREIRA, L. F.; VIEIRA, L. G. E.; PEREIRA, L. F. P. Identificação e caracterização de clones BAC de Coffea arabica L. HT 832/2 com marcas para resistência à ferrugem. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 6., 2009, Vitória. Inovação científica, competitividade e mudanças climáticas: anais. Vitória: Consórcio Pesquisa Café, 2009.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 5. | | YANAGUL, K.; VIEIRA, L. G.; POT, D.; PEREIRA, L. F. P. Avaliação da diversidade nucleotídica para identificação e análise de expressãode haplótipos em Coffea arabica (Nucleotide diversity evaluation for identification and expression analysis of Coffea arabica haplotypes) In: CONGRESO LATINOAMERICANO DE GENÉTICA, 14.; CONGRESO DE LA ASSOCIACIÓN LATINOAMERICANA DE MUTAGÊNESIS, CARCINOGÉNESES Y TERATOGÉNESIS AMBIENTAL, 8.; CONGRESO DE LA SOCIEDAD DE GENÉTICA DE CHILE,42.; CONGRESO DE LA SOCIEDAD ARGENTINA DE GENÉTICA, 39.; 2010, Vinã del Mar, Chile. p. 266| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 7. | | TISKI, I.; PEREIRA, L. F. P.; POT, D.; MARRACCINI, P. R.; VIEIRA, L. G. E. Análise in silico e in vivo da via de isoprenóides em café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Café. |
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| 8. | | TISKI, I.; PEREIRA, L. F. P.; POT, D.; MARRACCINI, P.; VIEIRA, L. G. E. Análise in silico e in vivo da via de isoprenóides em café. In: SIMPÓSIO DE PESQUISA DOS CAFÉS DO BRASIL, 5., 2007, Águas de Lindóia, SP. Anais... Brasília, DF: Embrapa Café, 2007. Biotecnologia aplicada à cadeia agroindustrial do café.| Tipo: Artigo em Anais de Congresso / Nota Técnica | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 10. | | SESSO, P. P.; PEREIRA, L. F. P.; SESSO FILHO, U. A.; ZAPPAROLI, I. D. Análise exploratória de dados espaciais da produção de café no estado do Paraná, 1980-2018. Cadernos de Ciência & Tecnologia, v. 37, n. 3, e26768, 2020. Título em inglês: Exploratory analysis of spatial data of the coffee production in Paraná state, 1980-2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Café; Embrapa Unidades Centrais. |
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| 20. | | ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. Coffee microspore cultivation to attain doubled-haploid plantlets. Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 180 | |
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