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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/03/2017 |
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Data da última atualização: |
29/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BALZON, T. A.; LUIS, Z. G.; PEREIRA, J. E. S. |
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Afiliação: |
Talita Aparecida Balzon; Zanderluce Gomes Luis, UnB; JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen. |
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Título: |
New approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In Vitro Cellular e Developmental Biology, v. 49, p. 41-50, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
We developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge. MenosWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embr... Mostrar Tudo |
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Palavras-Chave: |
Zygotic embryo. |
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Thesaurus Nal: |
morphogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180927/1/Balzon2013-Article-NewApproachesToImproveTheEffic.pdf
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Marc: |
LEADER 02287naa a2200169 a 4500
001 2067490
005 2023-03-29
008 2013 bl uuuu u00u1 u #d
100 1 $aBALZON, T. A.
245 $aNew approaches to improve the efficiency of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.) from mature zygotic embryos.$h[electronic resource]
260 $c2013
520 $aWe developed an efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from mature zygotic embryos of oil palm. Embryogenic calli were induced from mature zygotic embryos of oil palm on modified Murashige and Skoog medium with 2,4-dichlorophenoxyacetic acid or picloram, alone or in combination with activated charcoal. The greatest frequency of embryogenic callus induction (97.5%) was obtained by culturing mature zygotic embryos on callus induction medium with 450 μM picloram and 2.5 gL−1 activated charcoal. Embryogenic calli proliferated on a medium with a reduced concentration of picloram. Embryogenic calli were then subcultured on a medium supplemented with 12.3 μM 2-isopentenyladenine and 0.54 μM naphthaleneacetic acid, with subcultures at 4-wk intervals. Somatic embryos were regenerated on a medium with Murashige and Skoog macro- and micronutrients at halfstrength concentrations supplemented with 20 gL−1 sucrose, 2.5 gL−1 activated charcoal, and 2.5 gL−1 Phytagel. Detailed histological analysis revealed that somatic embryogenesis followed an indirect pathway. Primary calli were observed after 4?6 wk of culture and progressed to embryogenic calli at 12 wk. Embryogenic cells exhibited dense protoplasm, a high nucleoplasmic ratio, and small starch grains. Proembryos, which seemed to have a multicellular origin, formed after 16?20 wk of culture and successive cell divisions. Differentiated somatic embryos had a haustorium, a plumule, and the first and second foliar sheaths. In differentiated embryos, the radicular protrusion was not apparent because it generally does not appear until after the first true leaves emerge.
650 $amorphogenesis
653 $aZygotic embryo
700 1 $aLUIS, Z. G.
700 1 $aPEREIRA, J. E. S.
773 $tIn Vitro Cellular e Developmental Biology$gv. 49, p. 41-50, 2013.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 264 | |
| 22. |  | COSTA, F. H. da S.; PEREIRA, J. E. S. Avaliação da multiplicação in vitro da bananeira, cv. Grand Naine (AAA). In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA PIBIC/CNPq//UFAC, 14.; MOSTRA DE PESQUISA E PÓS-GRADUAÇÃO, 4., 2005, Rio Branco, AC. Anais... Rio Branco, AC: Ufac, 2005. 2 p. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| 25. | | LOUREIRO DA SILVA, T.; CAMILLO, J.; PEREIRA, J. E. S. Tolerância à dessecação e conservação sob temperatura sub-zero de sementes de Piper aducum L., uma espécie da Região Amazônica do Brasil. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 419-420.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 29. | | SILVA, R. C.; CAMILLO, J.; PEREIRA, J. E. S. Viabilidade na conservação de sementes de Pinhão Manso (Jatropha curcas L.) por criopreservação. Informativo Abrates, Londrina, v. 19, n. 2, p. 365, set. 2009. Edição dos resumos do XVI Congresso Brasileiro de Sementes, Curitiba, 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 36. | | CARVALHO SILVA, R. de; CAMILLO, J.; PEREIRA, J. E. S. Conservação a longo prazo de sementes de pinhão manso (Jatropha curcas L.) sob temperatura sub-zero. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 429-430.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 37. | | PEREIRA, J. E. S.; COSTA, F. H. da S. Conservação in vitro de recursos genéticos de plantas: estratégias, princípios e aplicações. In: BARRUETO CID, L. P. (Ed.). Cultivo in vitro de plantas. Brasília, DF: Embrapa Informação Tecnológica: Embrapa Recursos Genéticos e Biotecnologia, 2010. 303 p. il. color. Autores: Ana Cristina Miranda Brasileiro, Don J. Durzan, Eurico Eduardo Pinto de Lemos, Francisco J. L. Aragão, Frederico Henrique da Silva Costa, João Batista Teixeira, Jonny Everson Scherwinski-Pereira, L. Pedro Barrueto Cid, Maria Cristina Rocha Cordeiro, Miguel Jordan Zimmermann. p. 177-233| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 38. | | LOUREIRO DA SILVA, T.; PEREIRA, J. E. S. Criopreservação de sementes de Piper hispidinervum CD. C, uma espécie da Região Amazônica do Brasil. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMÉRICA LATINA Y EL CARIBE, SIRGEALC, 7., 2009, Pucón, Chile. Proceeding... Santiago de Chile: Ministério de Agricultura, Instituto de Investigaciones Agropecuarias, 2009. p. 421-422.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 39. | | PEREIRA, J. E. S.; FORTES, G. R. L. Determinação da concentração bactericida mínima inibitória de antibióticos para o controle de bactérias contaminantes em batata. Horticultura Brasileira, v. 23, n. 2. p.606, ago 2005. Suplemento. Edição dos resumos: CONGRESSO BRASILEIRO DE OLERICULTURA, 45.; CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 15.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 2., 2005, Fortaleza, CE.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 40. | | PEREIRA, J. E. S.; FORTES, G. R. de L. Determinação da concentração bactericida mínima inibitória de antibióticos para o controle de bactérias contaminantes em batata. Horticultura Brasileira, v. 23, n. 2. p. 606, ago 2005. Suplemento. Edição dos resumos: CONGRESSO BRASILEIRO DE OLERICULTURA, 45.; CONGRESSO BRASILEIRO DE FLORICULTURA E PLANTAS ORNAMENTAIS, 15.; CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 2., 2005, Fortaleza, CE.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Acre. |
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| Registros recuperados : 264 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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