Data/hora: 22/06/2024 03:41:03

Biblioteca(s): | Embrapa Mandioca e Fruticultura; Embrapa Unidades Centrais.
Data corrente: | 06/03/2015
Data da última atualização: | 13/05/2015
Autoria: | BASSO, M. F.; PEREIRA, A. J.; PEREIRA, H. M. de B.; RAMOS, H. J. de O.; DANTAS, J. L. L.; FONTES, E. P. B.; ANDRADE, E. C. de; ZERBINI, F. M.
Afiliação: | MARCOS FERNANDO BASSO; ALVARO JÚLIO PEREIRA; HERMANO MONTEIRO DE BARROS PEREIRA; HUMBERTO JOSUÉ DE OLIVEIRA RAMOS; JORGE LUIZ LOYOLA DANTAS; ELIZABETH PACHECO BATISTA FONTES; EDUARDO CHUMBINHO DE ANDRADE, SRI; FRANCISCO MURILO ZERBINI.
Título: | Screening of papaya accessions resistant to papaya lethal yellowing virus and capacity of Tetranychus urticae to transmit the virus.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira. Brasília, DF, v. 50, n. 2, p. 97-105. fev. 2015
ISSN: | 0100-204X
Idioma: | Inglês

Conteúdo:

The objective of this work was to produce a polyclonal antiserum against the coat protein (CP) of Papaya lethal yellowing virus (PLYV) and to determine its specificity and sensibility in the diagnosis of the virus, as well as to evaluate the genetic resistance to PLYV in papaya (Carica papaya) accessions and to investigate the capacity of the two?spotted spider mite etranychus urticae to acquire and transmit PLYV to the plants. Sixty?five papaya accessions were evaluated. For each accession, ten plants were mechanically inoculated using PLYV?infected plant extracts, and three plants were mock inoculated with phosphate buffer alone and used as negative controls. Ninety days after inoculation, newly?emerging systemic leaves were collected from the inoculated plants, and viral infection was diagnosed by indirect Elisa, using polyclonal antiserum sensible to the in vitro?expressed PLYV CP. Viral transmission by
T . urticae was evaluated in greenhouse. The experiments were repeated twice. Polyclonal antiserum recognized the recombinant PLYV CP specifically and discriminated PLYV infection from infections caused by other plant viruses. Out of the 65 papaya accessions evaluated, 15 were considered resistant, 18 moderately resistant, and 32 susceptible. The two?spotted spider mite T . urticae was capable of acquiring PLYV, but not of transmitting it to papaya.

Palavras-Chave: | Two-spotted spider mite.
Thesagro: | Carica Papaya; Mamão.
Thesaurus Nal: | Genetic resistance; Plant breeding.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/119743/1/Screening-of-papaya-accessions.pdf
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)

Exemplares:

AI-SEDE - (57367-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS
CNPMF - (30662-1) - UPC - AP - PP -  -  -  - DIS