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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
07/02/2019 |
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Data da última atualização: |
07/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PEREIRA, C. M.; CARVALHO, A. C. de; SILVA, F. R. da; MELENDEZ, M. E.; LESSA, R. C.; ANDRADE, V. C. C.; KOWALSKI, L. P.; VETTORE, A. L.; CARVALHO, A. L. |
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Afiliação: |
CLÁUDIA MARIA PEREIRA, A. C. Camargo Cancer Hospital, Ludwig Institute for Cancer Research, São Paulo; ANA CAROLINA DE CARVALHO, Barretos Cancer Hospital, Unifesp; FELIPE RODRIGUES DA SILVA, CNPTIA; MATIAS ELISEO MELENDEZ, Barretos Cancer Hospital; ROBERTA CARDIM LESSA, A. C. Camargo Cancer Hospital, Ludwig Institute for Cancer Research; VALÉRIA CRISTINA C. ANDRADE, Unifesp; LUIZ PAULO KOWALSKI, A. C. Camargo Cancer Hospital; ANDRÉ L. VETTORE, Ludwig Institute for Cancer Research, Unifesp; ANDRÉ LOPES CARVALHO, A. C. Camargo Cancer Hospital, Barretos Cancer Hospital, Barretos. |
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Título: |
In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
BMC Cancer, v. 18, p. 1-10, 2018. |
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DOI: |
https://doi.org/10.1186/s12885-018-4077-3 |
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Idioma: |
Inglês |
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Notas: |
Article number: 193. |
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Conteúdo: |
Abstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated in 60% (12/20) and 75% (15/20) of OSCC samples, respectively, and in HNSCC cell lines. The treatment of cell lines JHU-13 and FaDu with the demethylating agent 5'-aza-dC was efficient in restoring CA3 and FHL1 expression. TCGA expression and methylation data on OSCC confirms the downregulation of these genes in OSCC samples and also suggests that expression of CA3 and FHL1 is probably regulated by methylation. The downregulation of CA3 and FHL1 observed in silico was validated in HNSCC cell lines and OSCC samples, showing the feasibility of integrating different datasets to select differentially expressed genes in silico. Conclusions: These results showed that the downregulation of CA3 and FHL1 data observed in the ORESTES libraries was validated in HNSCC cell lines and OSCC samples and in a large cohort of samples from the TCGA database. Moreover, it suggests that expression of CA3 and FHL1 could probably be regulated by methylation having an important role the oral carcinogenesis. MenosAbstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated in 60% (12/20) and 75% (15/20) of OSCC samples, respectively, and in HNSCC cell lines. The treatment of cell lines JHU-13 and FaDu with the demethylating agent 5'-aza-dC was efficient in restoring CA3 and FHL1 expression. TCGA expression and methylation data on OSCC confirms the downregulation of these genes in OSCC samples and also suggests that expression of CA3 and FHL1 is probably regulated by methylation. The downregulation of CA3 and FHL1 observed in silico was validated in HNSCC cell lines and OSCC samples, showing the feasibility of integrating different datasets to select differentially expressed genes in silico. Conclusions: These results showed that the downregulation of CA3 and FHL1 data observed in the ORESTES l... Mostrar Tudo |
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Palavras-Chave: |
Carcinogênese; Expressão gênica; Open reading expressed sequence tags; Oral squamous cell carcinoma; ORESTES; Validação in silico; Validação in vitro. |
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Thesagro: |
Metilação. |
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Thesaurus Nal: |
Gene expression; Methylation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192300/1/AP-InVitro-Felipe.pdf
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Marc: |
LEADER 02831naa a2200361 a 4500
001 2105692
005 2020-01-07
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12885-018-4077-3$2DOI
100 1 $aPEREIRA, C. M.
245 $aIn vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer.$h[electronic resource]
260 $c2018
500 $aArticle number: 193.
520 $aAbstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated in 60% (12/20) and 75% (15/20) of OSCC samples, respectively, and in HNSCC cell lines. The treatment of cell lines JHU-13 and FaDu with the demethylating agent 5'-aza-dC was efficient in restoring CA3 and FHL1 expression. TCGA expression and methylation data on OSCC confirms the downregulation of these genes in OSCC samples and also suggests that expression of CA3 and FHL1 is probably regulated by methylation. The downregulation of CA3 and FHL1 observed in silico was validated in HNSCC cell lines and OSCC samples, showing the feasibility of integrating different datasets to select differentially expressed genes in silico. Conclusions: These results showed that the downregulation of CA3 and FHL1 data observed in the ORESTES libraries was validated in HNSCC cell lines and OSCC samples and in a large cohort of samples from the TCGA database. Moreover, it suggests that expression of CA3 and FHL1 could probably be regulated by methylation having an important role the oral carcinogenesis.
650 $aGene expression
650 $aMethylation
650 $aMetilação
653 $aCarcinogênese
653 $aExpressão gênica
653 $aOpen reading expressed sequence tags
653 $aOral squamous cell carcinoma
653 $aORESTES
653 $aValidação in silico
653 $aValidação in vitro
700 1 $aCARVALHO, A. C. de
700 1 $aSILVA, F. R. da
700 1 $aMELENDEZ, M. E.
700 1 $aLESSA, R. C.
700 1 $aANDRADE, V. C. C.
700 1 $aKOWALSKI, L. P.
700 1 $aVETTORE, A. L.
700 1 $aCARVALHO, A. L.
773 $tBMC Cancer$gv. 18, p. 1-10, 2018.
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Embrapa Agricultura Digital (CNPTIA) |
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| 85. |  | CATELLA, A. C.; SPACKI, V.; GINDRI, B. S.; BUENO, B.; ZUCCO, C. A. Características da pesca de iscas vivas na bacia do Alto Paraguai em Mato Grosso do Sul. In: SIMPÓSIO SOBRE RECURSOS NATURAIS E SOCIOECONÔMICOS DO PANTANAL, 6.; EVENTO DE INICIAÇÃO CIENTÍFICA DO PANTANAL, 1., 2013, Corumbá, MS. Desafios e soluções para o Pantanal: resumos. Corumbá: Embrapa Pantanal, 2013.| Tipo: Resumo em Anais de Congresso |
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| 86. | | VITAL, S.; SOARES, D. da C.; FERNANDES, J.; CATELLA, A. C. Captura de iscas vivas na comunidade do Porto da Manga, Rio Paraguai, Corumbá, MS. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA PANTANAL E UNIVERSIDADE FEDERAL DE MATO GROSSO DO SUL, 2.; 2008, Corumbá. Resumos...Corumbá: Embrapa Pantanal, 2008. (Embrapa Pantanal. Documentos, 95). 1 p.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 90. | | LEUCHTENBERGER, C.; RHEINGANTZ, M. L.; ZUCCO, C. A.; CATELLA, A. C.; MAGNUSSON, W. E.; MOURAO, G. Giant otter diet differs between habitats and from fisheries offtake in a large Neotropical floodplain. Journal of Mammalogy, v. 101, n. 6, p. 1650-1659, dec. 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pantanal. |
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| 91. | | CATELLA, A. C.; PEIXER, J.; COPATTI, A.; CAMPOS, F. L. R. Desembarque e decisões políticas sobre a pesca no Pantanal de Mato Grosso do Sul, Brasil. In: ENCONTRO BRASILEIRO DE ICTIOLOGIA, 17., 2007, Itajaí, SC. Resumos...Itajaí: UNIVALI, 2007. p. 451| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pantanal. |
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| 93. | | LIMA, V. S. N. C.; SILVA CRUZ, L. W.; CATELLA, A. C.; EATON, D. Resultados preliminares sobre as capturas e devoluções de peixes em pescarias esportivas no Rio Paraguai, MS, em 2003. In: SIMPÓSIO SOBRE RECURSOS NATURAIS E SÓCIO-ECONÔMICOS DO PANTANAL, 4., 2004, Corumbá, MS. Sustentabilidade regional: anais. Corumbá: Embrapa Pantanal: UCDB: UFMS: SEBRAE-MS, 2004. (CD-ROM).| Biblioteca(s): Embrapa Pantanal. |
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| 94. | | VARGAS, R. D.; LIMA, R. P.; MARQUES, D. K. S.; CATELLA, A. C.; MORAES, A. S. Pesca no Pantanal do Mato Grosso do Sul (Bacia do Alto Paraguai). In: MARQUES, D. K. S.; MORAES, A. S. (Ed.). Pesca e piscicultura no Pantanal: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa Informação Tecnológica; Corumbá: Embrapa Pantanal, 2010. (Coleção 500 perguntas, 500 respostas). p. 51-58.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Pantanal. |
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| 96. | | SILVA, M. A. C. da; BEZERRA, M. A. de O.; CATELLA, A. C. Práticas milenares de pesca no Pantanal. Corumbá: Embrapa Pantanal, 2010. 6 p. Artigo de Divulgação na Mídia (ADM 141) Formato eletronico. Disponivel em: Agência Pantanal, Meat World, Aqui e destaque, Canal do produtor, Infobibos, Rios Vivos,
Campo Grande News, Maracaju News, Conheça o Pantanal, MS Notícias, Agora MS.| Tipo: Artigo de Divulgação na Mídia |
| Biblioteca(s): Embrapa Pantanal. |
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| Registros recuperados : 142 | |
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