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| 6. |  | PEPE, K. B. F.; TULIO, R. H.; SILVA, K. da; ZANATTA, J. A.; RACHWAL, M. F. G. Density of ammonificators and nitrifiers bacteria in soils cultiv ated with pine and native forest in the South of Brazil. Pesquisa Florestal Brasileira, Colombo, v. 39, (nesp), e201902043, 2019. p. 671. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.| Biblioteca(s): Embrapa Florestas. |
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| 9. | | PEIXOTO, R. T. dos G.; SILVA, K. da; FERREIRA, T.; PARRON, L. M.; CAMPOS, I. B. de; GIAROLA, N. F. A.; FOGAÇA, A. M.; PAULA, A. L. de; PEPE, K. B. F.; DEMETRIO, W. C.; BECKER, R. K.; BRUZ, L. dos S. M.; SANTOS, A.; SÁTIRO, J. N. de O.; BROWN, G. G.; LACERDA, A. E. B. de. Indicadores de qualidade do solo em sistemas de produção de erva-mate sombreado, integrado e sob pleno sol: estudo de caso em Bituruna, PR. Colombo: Embrapa Florestas, 2022. 56 p. (Embrapa Florestas. Documentos, 383). Selo ODS 1, 2, 6, 12, 13 e 15.| Biblioteca(s): Embrapa Florestas; Embrapa Solos. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
03/08/2022 |
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Data da última atualização: |
03/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
OLIVEIRA, M. E. F.; ARRAIS, A. M.; MELLO, M. R. B. de; VERGANI, G. B.; FIGUEIRA, L. M.; ESTEVES, S. N.; PEREIRA, V. S. do A.; GARCIA, A. R.; BARTLEWSKI, P. M.; FONSECA, J. F. da. |
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Afiliação: |
MARIA EMILIA FRANCO OLIVEIRA, UNESP; ALINE MATOS ARRAIS, UFRRJ; MARCO ROBERTO BOURG DE MELLO, UFRRJ; GABRIEL BRUN VERGANI, UNESP; LUCAS MACHADO FIGUEIRA, UFF; SERGIO NOVITA ESTEVES, CPPSE; VERONICA SCHINAIDER DO A PEREIRA, CPPSE; ALEXANDRE ROSSETTO GARCIA, CPPSE; PAWEL MIECZYSLAW BARTLEWSKI, Ontario Veterinary College, University of Guelph; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Study of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 57, n. 4, p. 393-401, apr. 2022. |
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DOI: |
10.1111/rda.14077 |
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Idioma: |
Inglês |
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Conteúdo: |
The present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ?2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ?10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ?10 ovulations are main contributors to viable embryo production in Morada Nova ewes. MenosThe present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ?2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ?10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV... Mostrar Tudo |
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Palavras-Chave: |
Follicle Stimulating Hormone; In vivo embryo production; Naturalized sheep breed; Non surgical embryo recovery; NSER; Ovarian Follicle. |
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Thesaurus NAL: |
Corpus luteum; Estrus synchronization; Sheep; Superovulation. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02973naa a2200361 a 4500
001 2145184
005 2022-08-03
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1111/rda.14077$2DOI
100 1 $aOLIVEIRA, M. E. F.
245 $aStudy of the factors affecting embryo yields and quality in superovulated Morada Nova ewes that underwent non-surgical uterine flushing.$h[electronic resource]
260 $c2022
520 $aThe present study compared the outcomes of in vivo embryo production in Morada Nova ewes subjected to either 9-day (G-9SOV , n = 21) or 12-day (G-12SOV , n = 21) progesterone (P4 )-based estruses synchronization protocol coupled with superovulatory treatment with decreasing doses of porcine follicle-stimulating hormone (133 mg of pFSH given over 3 days). Non-surgical embryo recovery (NSER) was performed 6-7 days after the onset of oestrus. Total antral follicle count doubled from the first to the sixth pFSH dose in both groups (p < .05). Oestrus responses did not vary between the two groups of animals (95.2%). Corpora lutea (CL) were detected in 85.0% and 60.0% of ewes that previously manifested oestrus behaviour in G-9SOV and G-12SOV respectively. NSER was successfully completed in 86.2% of ewes that had CL (p > .05). The mean number of CL per ewe/successfully flushed donor ewe was greater (p < .05) in G-12SOV (12.3 ± 1.7/12.1 ± 1.9) than in G-9SOV (7.9 ± 1.4/8.2 ± 1.6). Mean numbers of retrieved blastocysts and viable embryos were greater (p > .05) in G-12SOV (5.8 ± 1.9 and 3.7 ± 1.7) than G-9SOV (3.5 ± 1.1 and 0.8 ± 0.3 respectively). The total follicle count (all follicles ?2 mm in diameter) at the sixth pFSH dose (at P4 -device removal) was positively correlated (p < .05) with the number of CL (r = .95) and viable embryos (r = .91) in G-12SOV . The ewes with ?10 Cl (48% of all flushed donors) yielded 80.5% of viable embryos. In summary: (a) Morada Nova ewes from G-12SOV group had better superovulatory responses compared with G-9SOV group; (b) total follicle count at the last pFSH dose was a good predictor of superovulatory responses only in the ewes primed with P4 for 12 days; and (c) animals with ?10 ovulations are main contributors to viable embryo production in Morada Nova ewes.
650 $aCorpus luteum
650 $aEstrus synchronization
650 $aSheep
650 $aSuperovulation
653 $aFollicle Stimulating Hormone
653 $aIn vivo embryo production
653 $aNaturalized sheep breed
653 $aNon surgical embryo recovery
653 $aNSER
653 $aOvarian Follicle
700 1 $aARRAIS, A. M.
700 1 $aMELLO, M. R. B. de
700 1 $aVERGANI, G. B.
700 1 $aFIGUEIRA, L. M.
700 1 $aESTEVES, S. N.
700 1 $aPEREIRA, V. S. do A.
700 1 $aGARCIA, A. R.
700 1 $aBARTLEWSKI, P. M.
700 1 $aFONSECA, J. F. da
773 $tReproduction in Domestic Animals$gv. 57, n. 4, p. 393-401, apr. 2022.
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