Data/hora: 25/05/2024 12:47:32

Biblioteca(s): 	Embrapa Meio Ambiente.	
Data corrente: 	18/12/2013	
Data da última atualização: 	28/02/2014	
Autoria: 	MELO, I. S. de; SANTOS, S. N.; ROSA, L. H.; PARMA, M. M.; SILVA, L. J.; QUEIROZ, S. C. do N. de; PELLIZARI, V. H.	
Afiliação: 	ITAMAR SOARES DE MELO, CNPMA; SUIKINAI NOBRE SANTOS, ESALQ-USP; LUIZ HENRIQUE ROSA, ICB-UFMG; MARCIA MARIA PARMA, CNPMA; LEONARDO J SILVA; SONIA CLAUDIA DO NASCIMENTO DE QUEIROZ, CNPMA; VIVAN HELENA PELLIZARI, IO-USP.	
Título: 	Isolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Extremophiles, Tokyo, v. 18, n. 1, p. 15-23, 2014.	
Idioma: 	Inglês	
Conteúdo: 	The Antarctic endophytic fungus (strain ITA1-CCMA 952) was isolated from the moss Schistidium antarctici found in Admiralty Bay, King George Island, Antarctica. Strain ITA1-CCMA 952 was assigned to the specie Mortierella alpina by phylogenetic analysis based on 18S rRNA gene sequences. This strain produces high levels of polyunsaturated fatty acids (PUFAs), including y-(gamma) linolenic acid and arachidonic acid, which when combined represents 48.3 % of the total fatty acid content. Fungal extracts demonstrated strong antioxidant activity with the EC50 value of 48.7 lg mL-1 and also a strong antibacterial activity, mainly against the following bacteria: Escherichia coli, with a MIC of 26.9 lg mL-1 and Pseudomonas aeruginosa and Enterococcus faecalis, both with a MIC of 107 lg mL-1. A GC-MS analysis of the chloroform fraction obtained from the crude extract revealed the presence of potential antimicrobials (Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) and Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(phenylmethyl)) as the major compounds. Therefore, the M. alpina strain ITA1-CCMA 952 is a promising fungus for the biotechnological production of antibiotics, antioxidant substances and PUFAs. This study highlights the need for more research in extreme environments, such as Antarctica.	
Palavras-Chave: 	Antibacterial activity; PUFAs; Pyrrolopyrazine alkaloids; Schistidium antarctici.	
Thesagro: 	Bactéria; Fungo.	
Thesaurus Nal: 	Antarctica; Antibacterial properties; Entomopathogenic fungi; Mortierella.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Meio Ambiente (CNPMA)	
Exemplares: 	CNPMA - (12857-1) - UPC - AP - DD -  -  -  - DIS