Data/hora: 22/09/2024 21:43:20

Biblioteca(s): 	Embrapa Pantanal.	
Data corrente: 	30/04/2003	
Data da última atualização: 	13/03/2017	
Autoria: 	FIGUEIREDO, J. F.; PELLEGRIN, A. O.; FOSCOLO, C. B.; MACHADO, R. P.; MIRANDA, K. L.; LAGE, A. P.	
Afiliação: 	UFMG/EV (Belo Horizonte, MG); EMBRAPA Pantanal (Corumba, MS).	
Título: 	Evaluation of direct fluorescent antibody test for the diagnosis of Bovine Genital Campylobacteriosis.	
Ano de publicação: 	2002	
Fonte/Imprenta: 	Revista Latinoamericana de Microbiologia, v.44, n.3/4, p.118-123, 2002.	
Idioma: 	Inglês	
Conteúdo: 	The direct fluorescent antibody test (DFAT) for the diagnosis of Bovine Genital Campylo- bacteriosis was assessed for its detectgion limit, observer effect, sensitivity and specificity. In addition, the specificity of the fluorescent conjugate was tested against Campylobacter sp., Arcobacter sp., Helicobacter sp., E. coli and other bacteria from the preputial flora. Ten - fold dilutions of C. fetus subs. venerealis NCTC 10354 in PBS or preputial washing with or without centrifugation were used. All experiments were done in duplicate by three observers. Positive and negative controls were included in each  assay. The detection li- mits of DFAT were 104 CFU/ml for PBS and non - centrifuged preputial washings and 102 CFU/ml for centrifuged preputial washing. There was no observer effect. The sensitivity and specificity of DFAT were 92.59% and 88.89%, respectively. The DFAT was observed to be sensitive, specific and the effect of experienced observers was minimal on test per- formance.	
Palavras-Chave: 	Antibody test; Bovine genital campylobacteriosis; Campilobacteriosis genital bobina; Diagnosis; Direct fluorescent; Imunofluorescencia direta; Lavado prepucial; Preputial washing.	
Thesagro: 	Campylobacter Fetus; Diagnostico.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Pantanal (CPAP)	
Exemplares: 	CPAP - (53340-1) - UPC - SP - PP - SP16463 -  - 16463 - DIS