Data/hora: 13/06/2024 10:01:34

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 06/11/2003
Data da última atualização: | 26/10/2018
Autoria: | ARCURI, P. B.; THONNEY, M. L.; SCHOFIELD, P.; PELL, A. N.
Afiliação: | PEDRO BRAGA ARCURI, CNPGL; Michael Larry Thonney, Cornell University/Departament of Animal Sciences; Peter Schofield, Cornell University/Departament of Animal Sciences; Alice Nelson Pell, Cornell University/Departament of Animal Sciences.
Título: | Polyethylene glycol and polyvinylpirrolidone effect on bacterial rRNA extraction and hybridization from cells exposed to tannins.
Ano de publicação: | 2003
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 9, p. 1073-1081, set. 2003
Idioma: | Inglês
Notas: | Título em português: Efeito  de  polietilenoglicol  e  polivinilpirrolidona  na  extração  e  hibridização de rRNA bacteriano de células expostas a taninos.
Conteúdo: | In order to detect fluctuations in ruminal microbial populations due to forage tannins using 16S ribosomal RNA (rRNA) probes, recovery of intact rRNA is required. The objective of this work was to evaluate the effect of polyethylene glycol (PEG) and polyvinylpirrolidone (PVP) on extraction of bacterial rRNA, in the presence of tannins from tropical legume forages and other sources, that hybridize with oligonucleotide probes. Ruminococcus albus 8 cells were exposed to 8 g/L tannic acid or 1 g/L condensed tannins extracted from Acacia angustissima, banana (Musa sp.) skin, Desmodium ovalifolium, red grape (Vitis vinifera) skin and Inga edulis, or no tannins. Cells were rinsed with Tris buffer pH 7 containing either 8% PEG or 6% PVP prior to cell lysis. Total RNA samples rinsed with either PEG or PVP migrated through denaturing agarose gels. The 16S rRNA bands successfully hybridized with a R. albus species-specific oligonucleotide probe, regardless of tannin source. The effect of rinsing buffers on the density of 16S rRNA bands, as well as on the hybridization signals was compared. There were significant effects (P<0.01) when the controls were compared to either buffer treatments due to tannin type, buffer used and the interaction of tannin type and buffer. The significant interaction indicates the influence of tannin type on the parameters evaluated.
Palavras-Chave: | ecologia microbiana.
Thesagro: | Nutrição Animal.
Thesaurus Nal: | animal nutrition; microbial ecology; Ruminococcus albus.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/25117/1/v38n9a08.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (25117-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS