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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
16/11/2021 |
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Data da última atualização: |
16/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SAVOLDI, I. R.; IBELLI, A. M. G.; CANTAO, M. E.; PEIXOTO, J. de O.; MORES, M. A. Z.; LAGOS, E. B.; LOPES, J. S.; ZANELLA, R.; LEDUR, M. C. |
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Afiliação: |
IGOR RICARDO SAVOLDI, UDESC/Chapecó; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; MARCOS ANTONIO ZANELLA MORES, CNPSA; ESSAMAI BRIZOLA LAGOS, UEPG; JADER SILVA LOPES, BRF/Curitiba; RICARDO ZANELLA, UPF; MONICA CORREA LEDUR, CNPSA. |
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Título: |
A joint analysis using exome and transcriptome data identifiescandidate polymorphisms and genes involved with umbilical hernia in pigs. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
BMC Genomics, v. 22, n. 818, 2021. |
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DOI: |
https://doi.org/10.1186/s12864-021-08138-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Umbilical Hernia (UH) is characterized by the passage of part of the intestine through the umbilical canal forming the herniary sac. There are several potential causes that can lead to the umbilical hernia such as bacterial infections, management conditions and genetic factors. Since the genetic components involved with UH are poorly understood, this study aimed to identify polymorphisms and genes associated with the manifestation of umbilical hernia in pigs using exome and transcriptome sequencing in a case and control design. Results: In the exome sequencing, 119 variants located in 58 genes were identified differing between normal and UH-affected pigs, and in the umbilical ring transcriptome, 46 variants were identified, located in 27 genes. Comparing the two methodologies, we obtained 34 concordant variants between the exome and transcriptome analyses, which were located in 17 genes, distributed in 64 biological processes (BP). Among the BP involved with UH it is possible to highlight cell adhesion, cell junction regulation, embryonic morphogenesis, ion transport, muscle contraction, within others. Conclusions: We have generated the first exome sequencing related to normal and umbilical hernia-affected pigs, which allowed us to identify several variants possibly involved with this disorder. Many of those variants present in the DNA were confirmed with the RNA-Seq results. The combination of both exome and transcriptome sequencing approaches allowed us to better understand the complex molecular mechanisms underlying UH in pigs and possibly in other mammals, including humans. Some variants found in genes and other regulatory regions are highlighted as strong candidates to the development of UH in pigs and should be further investigated. MenosAbstract: Background: Umbilical Hernia (UH) is characterized by the passage of part of the intestine through the umbilical canal forming the herniary sac. There are several potential causes that can lead to the umbilical hernia such as bacterial infections, management conditions and genetic factors. Since the genetic components involved with UH are poorly understood, this study aimed to identify polymorphisms and genes associated with the manifestation of umbilical hernia in pigs using exome and transcriptome sequencing in a case and control design. Results: In the exome sequencing, 119 variants located in 58 genes were identified differing between normal and UH-affected pigs, and in the umbilical ring transcriptome, 46 variants were identified, located in 27 genes. Comparing the two methodologies, we obtained 34 concordant variants between the exome and transcriptome analyses, which were located in 17 genes, distributed in 64 biological processes (BP). Among the BP involved with UH it is possible to highlight cell adhesion, cell junction regulation, embryonic morphogenesis, ion transport, muscle contraction, within others. Conclusions: We have generated the first exome sequencing related to normal and umbilical hernia-affected pigs, which allowed us to identify several variants possibly involved with this disorder. Many of those variants present in the DNA were confirmed with the RNA-Seq results. The combination of both exome and transcriptome sequencing approaches allowed ... Mostrar Tudo |
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Palavras-Chave: |
Defeitos congênitos; Hérnia umbilical; RNA sequencing; RNA-Seq; Sequenciamento de exoma; SNP. |
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Thesagro: |
Abacate; Genética Animal; Suíno. |
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Thesaurus Nal: |
Animal genetics; Sequence analysis; Single nucleotide polymorphism; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02916naa a2200385 a 4500
001 2136149
005 2021-11-16
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12864-021-08138-4$2DOI
100 1 $aSAVOLDI, I. R.
245 $aA joint analysis using exome and transcriptome data identifiescandidate polymorphisms and genes involved with umbilical hernia in pigs.$h[electronic resource]
260 $c2021
520 $aAbstract: Background: Umbilical Hernia (UH) is characterized by the passage of part of the intestine through the umbilical canal forming the herniary sac. There are several potential causes that can lead to the umbilical hernia such as bacterial infections, management conditions and genetic factors. Since the genetic components involved with UH are poorly understood, this study aimed to identify polymorphisms and genes associated with the manifestation of umbilical hernia in pigs using exome and transcriptome sequencing in a case and control design. Results: In the exome sequencing, 119 variants located in 58 genes were identified differing between normal and UH-affected pigs, and in the umbilical ring transcriptome, 46 variants were identified, located in 27 genes. Comparing the two methodologies, we obtained 34 concordant variants between the exome and transcriptome analyses, which were located in 17 genes, distributed in 64 biological processes (BP). Among the BP involved with UH it is possible to highlight cell adhesion, cell junction regulation, embryonic morphogenesis, ion transport, muscle contraction, within others. Conclusions: We have generated the first exome sequencing related to normal and umbilical hernia-affected pigs, which allowed us to identify several variants possibly involved with this disorder. Many of those variants present in the DNA were confirmed with the RNA-Seq results. The combination of both exome and transcriptome sequencing approaches allowed us to better understand the complex molecular mechanisms underlying UH in pigs and possibly in other mammals, including humans. Some variants found in genes and other regulatory regions are highlighted as strong candidates to the development of UH in pigs and should be further investigated.
650 $aAnimal genetics
650 $aSequence analysis
650 $aSingle nucleotide polymorphism
650 $aSwine
650 $aAbacate
650 $aGenética Animal
650 $aSuíno
653 $aDefeitos congênitos
653 $aHérnia umbilical
653 $aRNA sequencing
653 $aRNA-Seq
653 $aSequenciamento de exoma
653 $aSNP
700 1 $aIBELLI, A. M. G.
700 1 $aCANTAO, M. E.
700 1 $aPEIXOTO, J. de O.
700 1 $aMORES, M. A. Z.
700 1 $aLAGOS, E. B.
700 1 $aLOPES, J. S.
700 1 $aZANELLA, R.
700 1 $aLEDUR, M. C.
773 $tBMC Genomics$gv. 22, n. 818, 2021.
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Embrapa Suínos e Aves (CNPSA) |
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| Registros recuperados : 207 | |
| 21. |  | GRUPIONI, N. V.; VENTURINI, G. C.; IBELLI, A. M. G.; PEIXOTO, J. de O.; LEDUR, M. C.; MUNARI, D. P. Association of the Runt-related transcription factor 2 (RUNX2) gene with bone integrity traits in a paternal broiler line. In: INTERNATIONAL PLANT & ANIMAL GENOME, 22., 2014, San Diego, CA. Abstracts? San Diego, 2014. P642.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 22. | | GOLDONI, I.; IBELLI, A. M. G.; PEIXOTO, J. de O.; HUL, L. M.; CANTAO, M. E.; LEDUR, M. C. Análise comparativa de transcriptoma de frangos normais e afetados com necrose da cabeça do fêmur. In: ENCONTRO DE GENÉTICA DO NORDESTE, 23., 2021, Petrolina. Da produção de alimentos à saúde única: anais. Natal: UFRN: Sociedade Brasileira de Genética Regional Nordeste, 2021. Evento online.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 23. | | FREITAS, M. S. de; GARCIA, D. A.; LEDUR, M. C.; PEIXOTO, J. de O.; TORRES, R. J. de A. Avaliação da capacidade preditiva de metodologias de seleção genômica ampla em diferentes cenários simulados de arquiteturas genômicas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 52., 2015, Belo Horizonte. Zootecnia: otimizando recursos e potencialidades: anais. Brasília, DF: Sociedade Brasileira de Zootecnia, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 24. | | PERI, E.; FONGARO, G.; TESSMANN, A. L.; RIBEIRO, J. B.; PEIXOTO, J. de O.; LEDUR, M. C. Associação do marcador LEPR com composição da coxa em frango de corte. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA/UNC, 4., 2010, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves, 2010. 1 CD-ROM. 4 JINC. Projeto/Plano de Ação: 01.06.10.602-03.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 25. | | NEIS, K. L.; FORNARI, M. B.; MARCHESI, J. A. P.; FONGARO, G.; PEIXOTO, J. de O.; LEDUR, M. C. Associação de um marcador molecular no gene da Grelina com características de integridade óssea em frangos de corte. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA , 5., 2011, Concórdia. Resumos. Concórdia: Embrapa Suínos e Aves, 2011. p. 23 . JINC. Projeto/Plano de Ação: 06.09.06.001.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 26. | | MARCHESI, J. A. P.; FORNARI, M. B.; NEIS, K. L.; TESSMANN, A. L.; LEDUR, M. C.; PEIXOTO, J. de O. Associação do marcador molecular LEPR1 A>G com características de integridade óssea em frangos de corte. In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA , 5., 2011, Concórdia. Resumos. Concórdia: Embrapa Suínos e Aves, 2011. p. 22 . JINC. Projeto/Plano de Ação: 02.10.06.003| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 27. | | FORNARI, M. B.; NEIS, K. L.; MARCHESI, J. A. P.; LEDUR, M. C.; SOCCOL, V. T.; PEIXOTO, J. de O. Association of the A211G polymorphism in the bone sialoprotein gene with skeletal structure in a paternal broiler line. In: WORLD´S POULTRY CONGRESS, 24., 2012, Salvador. Abstract... Salvador: WSPA, 2012. 1 CD-ROM. World's Poultry Science Journal, v. 68, supl. 1, 2012. Projeto/Plano de Ação: 02.10.06.003.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 28. | | PEIXOTO, J. de O.; FARIA, D. A. de; SILVA, P. V.; FONSECA, I.; LOPES, P. S.; GUIMARÃES, S. E. F. Association between leptin genes single nucleotide polymorphisms and carcass traits in pigs Revista Brasileira de Zootecnia, v.38, n.2, p.271-276, 2009 Subprojeto: 16.00.30004-00| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 29. | | ZANELLA, R.; CANTAO, M. E.; PEIXOTO, J. de O.; PANDOLFI, J. R. C.; COUTINHO, L. L.; LEDUR, M. C. Collaborative effort to identify genes involved with bone related traits in broiler chickens. In: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. Abstracts... Jersey City: Scherago International, 2013. P0640.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 31. | | TEIXEIRA, S. T. F.; DICKEL, E. L.; REMOR, A.; JAENISCH, F. R. F.; PEIXOTO, J. de O.; DAROIT, L. Estudo anátomo-patológico do músculo pectoralis major de frangos de corte acometidos com white striping (WS). In: CONGRESSO BRASILEIRO DE MEDICINA VETERINÁRIA, 42; CONGRESSO SUL-BRASILEIRO DA ANCLIVEPA, 1., 2015, Curitiba. Anais... Curitiba: ANCLIVEPA-PR; SPRMV, 2015. p. 08-10. CONBRAVET.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 32. | | BONGARO, G.; PERI, E.; TESSMANN, A. L.; RIBEIRO, J. B.; PEIXOTO, J. de O.; LEDUR, M. C. Frequência alélica e genotípica de polimorfismo no gene receptor da leptina (LEPR) em duas linhagens de aves (Gallus gallus). In: JORNADA DE INICIAÇÃO CIENTÍFICA EMBRAPA/UNC, 4., 2010, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves, 2010. 1 CD-ROM. 4 JINC. Projeto/Plano de Ação: 01.06.10.602-03.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 34. | | CORSO, J.; HEPP, D.; LEDUR, M. C.; PEIXOTO, J. de O.; FAGUNDES, N. J. R.; FREITAS, T. R. O. Genetic variation of the bronze locus (MC1R) in turkeys from Southern Brazil. Genetics and Molecular Biology, v. 40, n. 1, p. 104-108, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 37. | | CRUZ, V. A. R.; VENTURINI, G. C.; PEIXOTO, J. de O.; LEDUR, M. C.; SCHMIDT, G. S.; MUNARI, D. P. Estimativas de parâmetros genéticos para peso corporal, gordura abdominal e peso de peles em linhagem pura de frangos de corte. In: CONFERÊNCIA FACTA DE CIÊNCIA E TECNOLOGIA AVÍCOLAS, 2011, Santos, SP. Anais... Santos: FACTA, 2011. Trabalhos de Pesquisa José Maria Lamas da Silva. 1 CD-ROM. Projeto: 02.09.07.006.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 39. | | SAVOLDI, I. R.; PETRY, B.; CARMO, K. B. do; IBELLI, A. M. G.; PEIXOTO, J. de O.; LEDUR, M. C. Expressão do gene PTHLH em frangos de corte normais e afetados pela necrose da cabeça do fêmur. In: JORNADA DE INICIAÇÃO CIENTÍFICA, 10., 2016, Concórdia. Anais... Concórdia: Embrapa Suínos e Aves: UNC, 2017. p. 13-14. JINC.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 207 | |
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