Data/hora: 21/06/2024 00:44:43

Biblioteca(s): 	Embrapa Agroenergia.	
Data corrente: 	23/09/2019	
Data da última atualização: 	23/09/2019	
Autoria: 	GREGORINE, L. F.; MONCLARO, A. V.; SILVA, C. de O. G.; RODRIGUES, K. B.; PEIXOTO, J. S. G.; ABDELNUR, P. V.; FAVARO, L. C. de L.	
Afiliação: 	Lucas F. Gregorine; Antonielle V. Monclaro; Caio de Oliveira Gorgulho Silva, Consultor da Embrapa Agroenergia; Kelly B. Rodrigues; Jéssica S. G. Peixoto, UnB; PATRICIA VERARDI ABDELNUR, CNPAE; LEIA CECILIA DE LIMA FAVARO, CNPAE.	
Título: 	Heterologous expression of a new lytic polysaccharide monooxygenase from Hahella ganghwensis and their functional characterization.	
Ano de publicação: 	2019	
Fonte/Imprenta: 	In: SIMPÓSIO NACIONAL DE BIOPROCESSOS, 22.; SIMPÓSIO DE HIDRÓLISE ENZIMÁTICA DE BIOMASSA, 13., 2019, Uberlandia, MG. [Anais ...]. São Paulo: Associação Brasileira de Engenharia Química, 2019.	
Idioma: 	Inglês	
Conteúdo: 	The powerful class of oxidative enzymes, lytic polysaccharide monooxygenases (LPMOs) - also named Auxiliary Activity (AA) - are able to oxidize recalcitrant polysaccharides on lignocellulosic biomass. In this work, we successfully expressed three catalytic domains from bacterial LPMOs in the yeast Komagataella phaffii: domain MdAA10.1-SD (from Moritella dasanensis), domain VmAA10.2-SD (from Verrucosispora maris), and domain HgAA10.1-SD (from Hahella ganghwensis). Heterologous expression was analyzed by SDS-PAGE, Western-Blot, and Dot-Blot, while functional activity of these proteins was investigated by a combination of mass spectrometric and chromatographic methods. The recombinant LPMO catalytic domain HgAA10.1-SD from H. ganghwensis, a C1-oxidizer, was able to promote an oxidative cleavage of phosphoric-acid swollen cellulose (PASC) substrate in the presence of ascorbic acid as an electron donor, showing its potential for cellulose depolymerization.	
Palavras-Chave: 	Chromatographic; Spectrometric.	
Categoria do assunto: 	--	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/202240/1/107193-786998-field-submission-fulltext-file1.pdf	
Registro original: 	Embrapa Agroenergia (CNPAE)	
Exemplares: 	CNPAE - (3494-1) - UPC - AA - DD -  -  -  - DIS