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Registro Completo |
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Biblioteca(s): |
Embrapa Agrossilvipastoril. |
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Data corrente: |
18/02/2019 |
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Data da última atualização: |
18/02/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
LIMA, L. R.; MOMBACH, M. A.; PEDREIRA, B. C. e; FERREIRA, D. C.; CARVALHO, P. de; AMORIM, K. R. R. de; WEIMER, P. J.; CABRAL, L. da S. |
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Afiliação: |
LENI RODRIGUES LIMA, UFMT, CUIABA, MT; MIRCÉIA ANGELE MOMBACH, UFMT, CUIABA, MT; BRUNO CARNEIRO E PEDREIRA, CPAMT; DANIELA CRISTINA FERREIRA, UFMT, CUIABA, MT; PERIVALDO DE CARVALHO, UFMT, CUIABA, MT; KARITHA REGIANE RIBEIRO DE AMORIM, UFMT, CUIABA, MT; PAUL JAMES WEIMER, UNIVERSITY OF WISCONSIN, MADISON, US; LUCIANO DA SILVA CABRAL, UFMT, CUIABA, MT. |
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Título: |
Methanogens and rumen protozoa in cattle during transition to high grain diets. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas. |
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Páginas: |
não paginado. |
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Idioma: |
Inglês |
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Conteúdo: |
The rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen. MenosThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) we... Mostrar Tudo |
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Thesaurus Nal: |
Archaea; Methanogens; Rumen microorganisms. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192971/1/2018-cpamt-bruno-pedreira-methanogens-rumen-protozoa-cattle-transition-grains-diets.pdf
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Marc: |
LEADER 03328nam a2200241 a 4500
001 2106183
005 2019-02-18
008 2018 bl uuuu u00u1 u #d
100 1 $aLIMA, L. R.
245 $aMethanogens and rumen protozoa in cattle during transition to high grain diets.$h[electronic resource]
260 $aIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 55.; CONGRESSO BRASILEIRO DE ZOOTECNIA, 28., 2018, Goiânia. Construindo saberes, formando pessoas e transformando a produção animal: anais. Viçosa: Sociedade Brasileira de Zootecnia; Brasília, DF: Associação Brasileira de Zootecnistas.$c2018
300 $anão paginado.
520 $aThe rumen is a very complex ecosystem in which there is a high diversity and concentration of rumen microbes. Animals, microbial and diets effects shape the rumen microbial population what make the rumen microbial manipulation a hard task to be done. There are many aspects about rumen microbial what the scientists and nutritionists would like to change, but the reduction in rumen methanogens and its activities is probably the first one. Considering the probable association between protozoa and rumen methanogens, this study aimed to evaluate their populations in the rumen of beef cattle during transition from a forage to high grains diets by qPCR. Four rumen fitted male Nellore Cattle were used, which were submit to diets from Brachiaria brizantha grass to high grains, from which rumen samples (solid and fluid) were collected when the diets were composed by zero (grazing animals), 50, 60, 70, 80 and 90% of concentrate on dry matter basis, as well as to get pH measurements. The DNA of rumen microbial population was isolated by using beads, phenol and chloroform, which makes possible to get high quantity and quality of DNA isolated. The DNA of rumen microbial population was amplified using groups primers (protozoa and methanogens) by qPCR method, which permits to calculate the genes copies numbers for every group. The rumen pH ranged from 6.51 to 5.73 for forage fed animals and for 90% concentrate fed animals, respectively. We found that protozoa numbers (log10 genes copies) were only reduced for animals fed with 90% concentrate diet (4.00) compared to other diets (forage, 50, 60, 70 and 80%, 5.07 log10 genes copies). The decrease on protozoa numbers may be related to rumen pH, in which protozoa numbers drop 21% only when rumen pH was lower that 5.8 (80% concentrate in the diets). However, the methanogens numbers were higher in forage fed animals (4.40 log10 genes copies) that all grains diets (3.71 log10 genes copies). Thus, there was not a clear association between protozoa and methanogens numbers in the rumen, what has also been observed in other studies done recently using molecular tools. Although we did not have any data about methane emissions in this study, we could speculate the higher concentrate diets (more than 50% concentrate), there would be a lower methane emission, considering the low methanogens numbers in the rumen.
650 $aArchaea
650 $aMethanogens
650 $aRumen microorganisms
700 1 $aMOMBACH, M. A.
700 1 $aPEDREIRA, B. C. e
700 1 $aFERREIRA, D. C.
700 1 $aCARVALHO, P. de
700 1 $aAMORIM, K. R. R. de
700 1 $aWEIMER, P. J.
700 1 $aCABRAL, L. da S.
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Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
04/03/2024 |
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Data da última atualização: |
11/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SANTOS, M. F.; SILVA, M. C.; FREITAS, T. M. S.; DIAS, J. M.; MOURA, M. I.; JULIANO, R. S.; FIORAVANTI, C. S.; CARMO, A. S. |
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Afiliação: |
M. F. SANTOS, UNIVERSIDADE DE GOIÁS; M. C. SILVA, UNIVERSIDADE FEDERAL DA GRANDE DOURADOS; T. M. S. FREITAS, CENTRO UNIVERSITÁRIO BRASÍLIA DE GOIÁS; J. M. DIAS, UNIVERSIDADE DE GOIÁS; M. I. MOURA, UNIVERSIDADE DE GOIÁS; RAQUEL SOARES JULIANO, CPAP; C. S. FIORAVANTI, UNIVERSIDADE DE GOIAS; A. S. CARMO, UNIVERSIDADE DE GOIAS. |
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Título: |
Identification of runs of homozygosity (ROHs) in curraleiro pé‑Duro and pantaneiro cattle breeds. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Tropical Animal Health and Production, v. 56, n. 92, 2024. |
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DOI: |
https://doi.org/10.1007/s11250-024-03933-z |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to identify and characterize runs of homozygosis (ROHs), genes involved in production characteristics and adaptation to tropical systems and to estimate the inbreeding coefficient of Curraleiro Pé-Duro (CPD) and Pantaneiro (PANT), two brazilian locally adapted cattle breeds. The results demonstrated that 79.25% and 54.29% of ROH segments were bigger than 8 Mb in CPD and PANT, respectively, indicating recent inbred matings in the studied population. Six homozygosis islands were identified simultaneously in both breeds, where 175 QTLs and 1072 genes previously described as associated with production traits are located. The inbreeding coefficient (FROH) estimated based on ROHs (FROH) showed that inbreeding is low (2 to 4%), which is different from expected for small populations such as locally adapted ones. |
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Palavras-Chave: |
Pantaneiro. |
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Thesagro: |
Bovinocultura; Raça. |
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Thesaurus NAL: |
Cattle breeds. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01625naa a2200265 a 4500
001 2162511
005 2024-04-11
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11250-024-03933-z$2DOI
100 1 $aSANTOS, M. F.
245 $aIdentification of runs of homozygosity (ROHs) in curraleiro pé‑Duro and pantaneiro cattle breeds.$h[electronic resource]
260 $c2024
520 $aThis study aimed to identify and characterize runs of homozygosis (ROHs), genes involved in production characteristics and adaptation to tropical systems and to estimate the inbreeding coefficient of Curraleiro Pé-Duro (CPD) and Pantaneiro (PANT), two brazilian locally adapted cattle breeds. The results demonstrated that 79.25% and 54.29% of ROH segments were bigger than 8 Mb in CPD and PANT, respectively, indicating recent inbred matings in the studied population. Six homozygosis islands were identified simultaneously in both breeds, where 175 QTLs and 1072 genes previously described as associated with production traits are located. The inbreeding coefficient (FROH) estimated based on ROHs (FROH) showed that inbreeding is low (2 to 4%), which is different from expected for small populations such as locally adapted ones.
650 $aCattle breeds
650 $aBovinocultura
650 $aRaça
653 $aPantaneiro
700 1 $aSILVA, M. C.
700 1 $aFREITAS, T. M. S.
700 1 $aDIAS, J. M.
700 1 $aMOURA, M. I.
700 1 $aJULIANO, R. S.
700 1 $aFIORAVANTI, C. S.
700 1 $aCARMO, A. S.
773 $tTropical Animal Health and Production$gv. 56, n. 92, 2024.
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Embrapa Pantanal (CPAP) |
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