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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
09/12/2021 |
Data da última atualização: |
09/12/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; MARIGUELA, V. C.; MONTASSIER, M. DE F. S.; MONTASSIER, H. J. |
Afiliação: |
PRISCILA DINIZ LOPES, UNESP; CINTIA HIROMI OKINO, CPPSE; FILIPE SANTOS FERNANDO, UNESP; CAREN PAVANI, UNESP; VIVIANE CASAGRANDE MARIGUELA, UNESP; MARIA DE FÁTIMA SILVA MONTASSIER, UNESP; HÉLIO JOSÉ MONTASSIER, UNESP. |
Título: |
Comparative evaluation of immune responses and protection of chitosan nanoparticles and oil-emulsion adjuvants in avian coronavirus inactivated vaccines in chickens. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Vaccines, v.9, n.12, 1457, 2021. |
ISSN: |
2076-393X |
DOI: |
https://doi.org/10.3390/vaccines9121457 |
Idioma: |
Inglês |
Conteúdo: |
Efficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce strong humoral and cell-mediated immune (CMI) responses and complete protection against IBV virulent infection, wherein single administration was characterized by high IgA antibody levels in the mucosa, whereas when associated with the Mass vaccine, the serum IgG antibody was predominantly observed. On the other hand, single administration of the oily vaccine presented poor humoral and CMI responses and consequently incomplete protection against virulent challenge, but when associated with the Mass vaccine, immune responses were developed, and complete protection against infection was observed. Both of our experimental vaccines were able to induce full protection against virulent IBV challenge. A single dose of AvCoV-CS vaccine was sufficient to achieve complete protection, while AvCoV-O required a previous priming by a Mass strain to complete the protection. MenosEfficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce strong humoral and cell-mediated immune (CMI) responses and complete protection against IBV virulent infection, wherein single administration was characterized by high IgA antibody levels in the mucosa, whereas when associated with the Mass vaccine, the serum IgG antibody was predominantly observed. On the other hand, single administration of the oily vaccine presented poor humoral and CMI responses and consequently incomplete protection against virulent challenge, but when associated with the Mass vaccine, immune responses were developed, and complete protection against infection was observed. Both of our experimental vaccines were able to induce full protection against virulent IBV challenge. A single dose of AvCoV-CS vacci... Mostrar Tudo |
Palavras-Chave: |
Avian infectious bronchitis; Cellular immune response; Delivery carrier adjuvant; Mucosal immunization; Oil adjuvant. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/228815/1/ComparativeEvaluationImmune.pdf
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Marc: |
LEADER 02553naa a2200277 a 4500 001 2137397 005 2021-12-09 008 2021 bl uuuu u00u1 u #d 022 $a2076-393X 024 7 $ahttps://doi.org/10.3390/vaccines9121457$2DOI 100 1 $aLOPES, P. D. 245 $aComparative evaluation of immune responses and protection of chitosan nanoparticles and oil-emulsion adjuvants in avian coronavirus inactivated vaccines in chickens.$h[electronic resource] 260 $c2021 520 $aEfficient vaccines are the main strategy to control the avian coronavirus (AvCoV), although several drawbacks related to traditional attenuated and inactivated vaccines have been reported. These counterpoints highlight the importance of developing new alternative vaccines against AvCoV, especially those able to induce long-lasting immune responses. This study evaluated and compared two inactivated vaccines formulated with AvCoV BR-I variants, one composed of chitosan nanoparticles (AvCoV-CS) and the second by Montanide oily adjuvant (AvCoV-O). Both developed vaccines were administered in a single dose or associated with the traditional Mass attenuated vaccine. The AvCoV-CS vaccine administered alone or associated with the Mass vaccine was able to induce strong humoral and cell-mediated immune (CMI) responses and complete protection against IBV virulent infection, wherein single administration was characterized by high IgA antibody levels in the mucosa, whereas when associated with the Mass vaccine, the serum IgG antibody was predominantly observed. On the other hand, single administration of the oily vaccine presented poor humoral and CMI responses and consequently incomplete protection against virulent challenge, but when associated with the Mass vaccine, immune responses were developed, and complete protection against infection was observed. Both of our experimental vaccines were able to induce full protection against virulent IBV challenge. A single dose of AvCoV-CS vaccine was sufficient to achieve complete protection, while AvCoV-O required a previous priming by a Mass strain to complete the protection. 653 $aAvian infectious bronchitis 653 $aCellular immune response 653 $aDelivery carrier adjuvant 653 $aMucosal immunization 653 $aOil adjuvant 700 1 $aOKINO, C. H. 700 1 $aFERNANDO, F. S. 700 1 $aPAVANI, C. 700 1 $aMARIGUELA, V. C. 700 1 $aMONTASSIER, M. DE F. S. 700 1 $aMONTASSIER, H. J. 773 $tVaccines$gv.9, n.12, 1457, 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registros recuperados : 3 | |
1. | | LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; MARIGUELA, V. C.; MONTASSIER, M. DE F. S.; MONTASSIER, H. J. Comparative evaluation of immune responses and protection of chitosan nanoparticles and oil-emulsion adjuvants in avian coronavirus inactivated vaccines in chickens. Vaccines, v.9, n.12, 1457, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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2. | | LOPES, P. D.; OKINO, C. H.; FERNANDO, F. S.; PAVANI, C.; CASAGRANDE, V. M.; LOPEZ, R. F. V.; MONTASSIER, M. F. de; MONTASSIER, H. J. Inactivated infectious bronchitis virus vaccine encapsulated in chitosan nanoparticles induces mucosal immune responses and effective protection against challenge. Vaccine, v. 36, p. 2630-2636, abr. 2018. 36Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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3. | | SANTOS, R. M. dos; FERNANDO, F. S.; MONTASSIER, M. de F. S.; SILVA, K. R.; LOEPS, P. D.; PAVANI, C.; BORZI, M. M.; OKINO, C. H.; MONTASSIER, H. J. Memory immune responses and protection of chickens against a nephropathogenic infectious bronchitis virus strain by combining live heterologous and inactivated homologous vaccines. The Journal of Veterinary Medical Science, v.81, n.4, p.612-619, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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Registros recuperados : 3 | |
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