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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/01/1998 |
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Data da última atualização: |
12/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TORRES, G. A.; PARENTONI, S. N.; LOPES, M. A.; PAIVA, E. |
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Afiliação: |
EMBRAPA/CNPMS; SIDNEY NETTO PARENTONI, CNPMS. |
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Título: |
A search for RFLP markers to identify genes for aluminum tolerance in maize. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Revista Brasileira de Genética, Ribeirão Preto, v. 20, n. 3, p. 459-465, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8. MenosThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested f... Mostrar Tudo |
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Palavras-Chave: |
Maize; RFLP; Tolerance; Tolerancia. |
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Thesagro: |
Alumínio; Milho; Zea Mays. |
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Thesaurus Nal: |
aluminum. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/43456/1/Search-RFLP.pdf
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Marc: |
LEADER 02698naa a2200253 a 4500
001 1478779
005 2018-06-12
008 1997 bl uuuu u00u1 u #d
100 1 $aTORRES, G. A.
245 $aA search for RFLP markers to identify genes for aluminum tolerance in maize.$h[electronic resource]
260 $c1997
520 $aThe objective of this study was to identify restriction fragment length polymorphism (RFLP) markers linked to QTLs that control aluminum (Al) tolerance in maize. The strategy used was bulked segregation analysis (BSA) and the genetic material utilized was an F2 population derived from a cross between the Al-susceptible inbred line L53 and Al-tolerant inbred line L1327. Both lines were developed at the National Maize and Sorghum Research Center - CNPMS/EMBRAPA. The F2 population of 1554 individuals was evaluated in a nutrient solution containing a toxic concentration of Al and relative seminal root length (RSRL) was used as a phenotype measured tolerance. The RSRL frequency distribution was continuous, but skewed towards Al-susceptible individuals. Seedlings of the F2 population which scored the highest and the lowest RSRL values were transplanted to the field and subsequently selfed to obtain F3 families. Thirty F3 families (15 Al-susceptible and 15 Al-tolerant) were evaluated in nutrient solution, using an incomplete block design, to identify those with the smallest variances for aluminum tolerance and susceptibility. Six Al-susceptible and five Al-tolerant F3 families were chosen to construct one pool of Al-susceptible individuals, and another of Al-tolerant, herein refered as "bulks", based on average values of RSRL and genetic variance. One hundred and thirteen probes were selected, with an average interval of 30 cM, covering the 10 maize chromosomes. These were tested for their ability to discriminate the parental lines. Fifty-four of these probes were polymorphic, with 46 showing codominance. These probes were hybridized with DNA from the two contrasting bulks. Three RFLPs on chromosome 8 distinguished the bulks on the basis of band intensity. DNA of individuals from the bulks was hybridized with these probes and showed the presence of heterozygous individuals in each bulk. These results suggest that in maize there is a region related to aliminum tolerance on chromosome 8.
650 $aaluminum
650 $aAlumínio
650 $aMilho
650 $aZea Mays
653 $aMaize
653 $aRFLP
653 $aTolerance
653 $aTolerancia
700 1 $aPARENTONI, S. N.
700 1 $aLOPES, M. A.
700 1 $aPAIVA, E.
773 $tRevista Brasileira de Genética, Ribeirão Preto$gv. 20, n. 3, p. 459-465, 1997.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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| Registros recuperados : 40 | |
| 3. |  | OLIVEIRA, E. A. de; RODERJAN, C. V.; CURCIO, G. R.; SILVA, S. M. Caracterização florística, fitossociológica e pedológica de um trecho de floresta ripária dos Campos Gerais do Paraná. Cadernos da Biodiversidade, Curitiba, v. 4, n. 1, p. 8-25, jan. 2003.| Biblioteca(s): Embrapa Florestas. |
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| 7. | | PRANDO, A. M.; ZUCARELI, C.; FRONZA, V.; OLIVEIRA, E. A. de P.; PANOFF, B. Formas de ureia e doses de nitrogênio em cobertura na qualidade fisiológica de sementes de trigo. Revista Brasileira de Sementes, Viçosa, MG, v. 34, n. 2, p. 272-279, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Soja. |
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| 10. | | FERNANDES, A. R. M.; SAMPAIO, A. A. M.; OLIVEIRA, E. A. de; TULLIO, R. R.; OLIVEIRA, T. V. de. Avaliação bioeconômica da terminação de bovinos Canchim, machos e fêmeas, em confinamento. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., Jaboticabal, SP. Anais... Jaboticabal: SBZ: UNESP, 2007.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 11. | | FERNANDES, A. R.; SAMPAIO, A. A. M.; WIGNEZ, H.; OLIVEIRA, E. A. de; TULLIO, R. R.; PIVARO, T. Composição dos lipídios intramusculares de bovinos Canchim terminados em confinamento, recebendo dietas com silagem de milho ou cana-de-açúcar e grãos de girassol. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 44., Jaboticabal, SP. Anais... Jaboticabal: SBZ: UNESP, 2007. 1 CD-ROM| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | MOURA, M. A.; RANZANI-PAIVA, M.J.T.; OLIVEIRA, E. A. de; CLEMENTE, Z.; VALLIM, J. H.; JONSSON, C. M. Toxicity of misture herbicides used in sugarcane crops to juvenile tilapias (Oreochromis niloticus) In: SETAC WORLD CONGRESS EUROPE, 23., 2013, Glasgow. Proceedings... Glasgow: SETAC, 2013. p. 343| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 15. | | OLIVEIRA, E. A. de; BORGES, S. V.; FURTADO, A. A. L.; DELLA MODESTA, R. C.; GODOY, R. de O. Heat processing (HTST) of umbu (Spondias tuberosa Arruda Câmara) pulp. Ciência e Tecnologia de Alimentos, Campinas, v. 31, n. 4, p. 923-928, out./dez. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 16. | | MONTEIRO, A. N. T. R.; BERTOL, T. M.; OLIVEIRA, E. A. de; SCADOLERA, A. J.; WARPECHOWSKI, M. B. Predição do pH final da carne suína a partir de parâmetros de qualidade mensurados 12 horas após o abate. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília, DF. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012. 1 CD-ROM. Projeto/Plano de Ação: 03.10.01.016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 17. | | OLIVEIRA, E. A. de; BERTOL, T. M.; COLDEBELLA, A.; SANTOS FILHO, J. I. dos; SCANDOLERA, A. J.; WARPECHOWSKI, M. B. Live performance, carcass quality, and economic assessment of over 100kg slaughtered pigs. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 67, n. 6, p. 1743-1750, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 18. | | MOURA, M. A.; RANZANI-PAIVA, M.J.T.; OLIVEIRA, E. A. de; CLEMENTE, Z.; VALLIM, J. H.; JONSSON, C. M. Effects of a mixture of herbicides on non-target organisms. In: SETAC WORLD CONGRESS EUROPE, 23., 2013, Glasgow. Proceedings... Glasgow: SETAC, 2013. p. 357.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | OLIVEIRA, E. A. de; BERTOL, T. M.; FIGUEIREDO, E. A. P. de; COLDEBELLA, A.; VIEIRA, M. S.; WARPECHOWSKI, M. B. Meat and fat deposition in Moura crossbred pigs slaughtered between 22 and 130 kg. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 53, 2016, Gramado. Produção animal para as novas gerações. Anais ... Gramado: SBZ, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 20. | | CHAIMSOHN, F. P.; CURCIO, G. R.; DEDECECK, R.; RACHWAL, M. F. G.; OLIVEIRA, E. A. de; GOMES, E. P.; BENASSI, D. A. SILVA, F. de A. E. Avaliação de bracatinga comum (Mimosa scabrella) e Bracatinga-de-Campo Mourão (M. flocculosa) em cambissolo alico para recuperação de áreas erodidas / degradadas. In: SIMPOSIO BRASILEIRO DE RECUPERACAO DE AREAS DEGRADADAS, 3., 1997, Ouro Preto. Do substrato ao solo: trabalhos voluntarios. Vicosa: Sociedade Brasileira de Recuperacao de Areas Degradadas: Universidade Federal de Vicosa, Departamento de Solos, 1997. p.350-354.| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 40 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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