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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
25/01/2017 |
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Data da última atualização: |
25/01/2017 |
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Autoria: |
SARKAR, P.; SINGH, S. P.; PANDEY, A. K.; KATHURIA, B. K.; KUMAR, S. |
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Título: |
Application of fluorescent antibody test in the diagnosis of sheep-pox, and study of sheep-pox virus multiplication in cell-culture. |
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Ano de publicação: |
1980 |
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Fonte/Imprenta: |
The Indian Journal of Animal Sciences, v. 50, n. 5, p. 428-433, May. 1980. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract : Direct fluorescent antibody test (FAT) was used to detect the sheep pox virus (SPV) in the oedema fluid of infected sheep, with or without traces of tissue fibres. Bright apple-green fluorescence against a dark-grey background was observed under the fluorescence microscope. The virus multiplied in monolayer cultures of lamb kidney cells. Depending upon the aggregation of virus particles, the size and brightness of the fluoresced masses became gradually prominent, and covered considerable areas of the cell cytoplasm. By indirect FAT, antibodies against SPV were detected with precision by adopting a two-stage staining procedure. This method can be used to detect the antibody status of a flock. |
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Palavras-Chave: |
Vírus da varíola ovina. |
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Thesagro: |
Doença animal; Ovino. |
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Thesaurus Nal: |
Animal diseases; Animal production; Capripoxvirus. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 01445naa a2200241 a 4500
001 2061753
005 2017-01-25
008 1980 bl uuuu u00u1 u #d
100 1 $aSARKAR, P.
245 $aApplication of fluorescent antibody test in the diagnosis of sheep-pox, and study of sheep-pox virus multiplication in cell-culture.
260 $c1980
520 $aAbstract : Direct fluorescent antibody test (FAT) was used to detect the sheep pox virus (SPV) in the oedema fluid of infected sheep, with or without traces of tissue fibres. Bright apple-green fluorescence against a dark-grey background was observed under the fluorescence microscope. The virus multiplied in monolayer cultures of lamb kidney cells. Depending upon the aggregation of virus particles, the size and brightness of the fluoresced masses became gradually prominent, and covered considerable areas of the cell cytoplasm. By indirect FAT, antibodies against SPV were detected with precision by adopting a two-stage staining procedure. This method can be used to detect the antibody status of a flock.
650 $aAnimal diseases
650 $aAnimal production
650 $aCapripoxvirus
650 $aDoença animal
650 $aOvino
653 $aVírus da varíola ovina
700 1 $aSINGH, S. P.
700 1 $aPANDEY, A. K.
700 1 $aKATHURIA, B. K.
700 1 $aKUMAR, S.
773 $tThe Indian Journal of Animal Sciences$gv. 50, n. 5, p. 428-433, May. 1980.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | ANGELIS, S. de; NOVAK, A. C.; SYDNEY, E. B.; SOCCOL, V. T.; CARVALHO, J. C.; PANDEY, A.; NOSEDA, M. D.; THOLOZAN, J. L.; LORQUIN, J.; SOCCOL, C. R. Co-culture of microalgae, cyanobacteria, and macromycetes for exopolysaccharides production: process preliminary optimization and partial characterization. Applied Biochemistry and Biotechnology, v. 167, n. 5, p. 1092-1106, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Florestas. |
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