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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
01/09/2000 |
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Data da última atualização: |
09/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WEID, I. von der; PAIVA, E.; NOBREGA, A.; ELSAS, J. D. van; SELDIN, L. |
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Afiliação: |
EMBRAPA-CNPMS. |
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Título: |
Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in cerrado soil. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Research in Microbiology, Paris, v. 151, n. 5, p. 369-381, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
Paenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX-PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX-PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair groups method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, respectively. Finally, all phenotypic and genetic data, or just the genetic data, were used in a multivariate analysis of variance (MANOVA) in order to address the heterogeneity among P. polymyxa populations during the different stages of maize growth. The resulting data showed that strains isolated 10, 30, 60 and 90 days after maize sowing were statistically different. MenosPaenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX-PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX-PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair groups method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, res... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genetica; Diversity; Maize. |
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Thesagro: |
Cerrado; Milho; Rizosfera; Zea Mays. |
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Thesaurus Nal: |
Paenibacillus polymyxa; rhizosphere. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02652naa a2200277 a 4500
001 1484150
005 2018-06-09
008 2000 bl uuuu u00u1 u #d
100 1 $aWEID, I. von der
245 $aDiversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in cerrado soil.$h[electronic resource]
260 $c2000
520 $aPaenibacillus polymyxa populations present in the rhizosphere of maize (cultivar BR-201) planted in Cerrado soil were investigated in order to assess their diversity at four stages of plant growth. A total of 67 strains were isolated and all strains were identified as P. polymyxa by classical biochemical tests, API 50CH tests and a set of species-specific primers based on the 23S rDNA sequence. To compare the isolated strains, phenotypic characteristics (utilization of different carbohydrates, resistance to antibiotics and production of antimicrobial substances) and genetic approaches (hybridization with a Klebsiella pneumoniae nifKDH probe and BOX-PCR) were used. Fermentation of glycerol, arabinose, xylose and rhamnose varied among the isolates and these data divided the strains into five groups. Fifty strains (75%) showed homology to plasmid pSA30 (containing the nifKDH genes) resulting in five different hybridization patterns. Using BOX-PCR, 18 groups were observed. Phenetic analyses were applied based on the unweighted pair groups method with arithmetic means using the phenotypic and genetic data, separately. All P. polymyxa isolates could be divided into two main clusters at approximately 52% and into 18 groups at approximately 89% of similarity, when phenotypic data were used. Also, two main clusters were formed at 65% of similarity when genetic data were used. In this dendrogram, clusters were further split into 10 and 22 groups, at about 88 and 97% of similarity, respectively. Finally, all phenotypic and genetic data, or just the genetic data, were used in a multivariate analysis of variance (MANOVA) in order to address the heterogeneity among P. polymyxa populations during the different stages of maize growth. The resulting data showed that strains isolated 10, 30, 60 and 90 days after maize sowing were statistically different.
650 $aPaenibacillus polymyxa
650 $arhizosphere
650 $aCerrado
650 $aMilho
650 $aRizosfera
650 $aZea Mays
653 $aDiversidade genetica
653 $aDiversity
653 $aMaize
700 1 $aPAIVA, E.
700 1 $aNOBREGA, A.
700 1 $aELSAS, J. D. van
700 1 $aSELDIN, L.
773 $tResearch in Microbiology, Paris$gv. 151, n. 5, p. 369-381, 2000.
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Embrapa Milho e Sorgo (CNPMS) |
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Cutter |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/08/2011 |
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Data da última atualização: |
17/04/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEDROSA, F. O.; MONTEIRO, R. A.; WASSEM, R.; CRUZ, L. M.; AYUB, R. A.; COLAUTO, N. B.; FERNANDEZ, M. A.; FUNGARO, M. H. P.; GRISARD, E. C.; HUNGRIA, M.; MADEIRA, H. M. F.; NODARI, R. O.; OSAKU, C. A.; PETZL-ERLER, M. L.; TERENZI, H.; VIEIRA, L. G. E.; STEFFENS, M. B. R.; WEISS, V. A.; PEREIRA, L. F. P.; ALMEIDA, M. I. M.; ALVES, L. R.; MARIN, A.; ARAUJO, L. M.; BALSANELLI, E.; BAURA, V. A.; CHUBATSU, L. S.; FAORO, H.; FAVETTI, A.; FRIEDERMANN, G.; GLIENKE, C.; KARP, S.; KAVA-CORDEIRO, V.; RAITTZ, R. T.; RAMOS, H. J. O.; RIBEIRO, E. M. S. F.; RIGO, L. U.; ROCHA, S. N.; SCHWAB, S.; SILVA, A. G.; SOUZA, E. M.; MICHELLE Z. TADRA-SFEIR; TORRES, R. A.; DABUL, A. N. G.; SOARES, M. A. M.; GASQUES, L. S.; GIMENES, C. C. T.; VALLE, J. S.; CIFERRI, R. R.; CORREA, L. C.; MURACE, N. K.; PAMPHILE, J. A.; PATUSSI, E. V.; PRIOLI, A. J.; PRIOLI, S. M. A.; ROCHA, C. L. M. S. C.; ARANTES, O. M. N.; FURLANETO, M. C.; GODOY, L. P.; OLIVEIRA, C. E. C.; SATORI, D.; VILAS-BOAS, L. A.; WATANABE, M. A. E.; DAMBROS, B. P.; GUERRA, M. P.; MATHIONI, S. M.; SANTOS, K. L.; STEINDEL, M.; VERNAL, J.; BARCELLOS, F. G.; CAMPO, R. J.; CHUEIRE, L. M. O.; NICOLÁS, M. F.; PEREIRA-FERRARI, L.; SILVA, J. L. da C.; GIOPPO, N. M. R.; MARGARIDO, V. P.; MENCK-SOARES, M. A.; PINTO, F. G. S.; SIMÃO, R. de C. G.; TAKAHASHI, E. K.; YATES, M. G.; SOUZA, E. M. |
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Afiliação: |
FÁBIO O. PEDROSA, UFPR; ROSE ADELE MONTEIRO, UFPR; ROSELI WASSEM, UFPR; LEONARDO M. CRUZ, UFPR; RICARDO A. AYUB, UEPG; NELSON B. COLAUTO, Universidade Paranaense, Umuarama.; MARIA APARECIDA FERNANDEZ, UEM; MARIA HELENA P. FUNGARO, UEL; EDMUNDO C. GRISARD, UFSC; MARIANGELA HUNGRIA DA CUNHA, CNPSO; HUMBERTO M. F. MADEIRA8,, PUC Curitiba; RUBENS O. NODARI, UFSC; CLARICE A. OSAKU, UNIOESTE; MARIA LUIZA PETZL-ERLER, UFPR; HERNÁN TERENZI, UFSC; LUIZ G. E. VIEIRA, IAPAR; MARIA BERENICE R. STEFFENS, UFPR; VINICIUS A. WEISS, UFPR; LUIZ F. P. PEREIRA, IAPAR; MARINA I. M. ALMEIDA, UFPR; LYSANGELA R. ALVES, UFPR; ANELIS MARIN, UFPR; LUIZA MARIA ARAUJO, UFPR; EDUARDO BALSANELLI, UFPR; VALTER A. BAURA, UFPR; LEDA S. CHUBATSU, UFPR; HELISSON FAORO, UFPR; AUGUSTO FAVETTI, UFPR; GERALDO FRIEDERMANN, UFPR; CHIRLEI GLIENKE, UFPR; SUSAN KARP, UFPR; VANESSA KAVA-CORDEIRO, UFPR; ROBERTO T. RAITTZ, UFPR; HUMBERTO J. O. RAMOS, UFPR; ENILZE MARIA S. F. RIBEIRO, UFPR; LIU UN RIGO, UFPR; SAUL N. ROCHA, UFPR; STEFAN SCHWAB, UFPR; ANILDA G. SILVA, UFPR; ELIEL M. SOUZA, UFPR; TADRA-SFEIR, M. Z., UFPR; RODRIGO A. TORRES, UFPR; AUDREI N. G. DABUL, UEPG; MARIA ALBERTINA M. SOARES, UEPG; LUCIANO S. GASQUES, Universidade Paranaense, Umuarama; CIELA C. T. GIMENES, Universidade Paranaense, Umuarama.; JULIANA S. VALLE, Universidade Paranaense, Umuarama.; RICARDO R. CIFERRI, UEM; LUIZ C. CORREA, UEM; NORMA K. MURACE, UEM; JOÃO A. PAMPHILE, UEM; ELIANA VALÉRIA PATUSSI, UEM; ALBERTO J. PRIOLI, UEM; SONIA MARIA A. PRIOLI, UEM; CARMEM LÚCIA M. S. C. ROCHA, UEM; OLÍVIA MÁRCIA N. ARANTES, UEL; MÁRCIA CRISTINA FURLANETO, UEL; LEANDRO P. GODOY, UEL; CARLOS E. C. OLIVEIRA, UEL; DANIELE SATORI, UEL; LAURIVAL A. VILAS-BOAS, UEL; MARIA ANGÉLICA E. WATANABE, UEL; BIBIANA PAULA DAMBROS, UFSC; MIGUEL P. GUERRA, UFSC; SANDRA MARISA MATHIONI, UFSC; KARINE LOUISE SANTOS, UFSC; MARIO STEINDEL, UFSC; JAVIER VERNAL, UFSC; FERNANDO G. BARCELLOS, CNPSo - Pós-graduando; RUBENS J. CAMPO, CNPSo - Pesquisador aposentado; LIGIA MARIA DE OLIVEIRA CHUEIRE, CNPSO; MARISA FABIANA NICOLÁS, CNPSo - Pós-graduanda; LILIAN PEREIRA-FERRARI, PUC Curitiba-PR; JOSÉ L. DA CONCEICÃO SILVA, UNIOESTE; NEREIDA M. R. GIOPPO, UNIOESTE; VLADIMIR P. MARGARIDO, UNIOESTE; MARIA AMÉLIA MENCK-SOARES, UNIOESTE; FABIANA GISELE S. PINTO, UNIOESTE; RITA DE CÁSSIA G. SIMÃO, UNIOESTE; ELIZABETE K. TAKAHASHI, IAPAR; MARSHALL G. YATES, UFPR; EMANUEL M. SOUZA, UFPR. |
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Título: |
Genome of Herbaspirillum seropedicae Strain SmR1, a specialized diazotrophic endophyte of tropical grasses. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
PLoS Genetics, v. 7, n. 5, p. 1-10, may 2011. |
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DOI: |
10.1371/journal.pgen.1002064 |
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Idioma: |
Português |
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Conteúdo: |
The molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme?GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species. |
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Palavras-Chave: |
Fixação nitrogênio. |
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Thesagro: |
Genoma; Graminea tropical. |
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Thesaurus NAL: |
Genome; Grasses; Herbaspirillum seropedicae; Nitrogen fixation. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/39544/1/plos-genetics.pdf
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Marc: |
LEADER 04596naa a2201189 a 4500
001 1897676
005 2018-04-17
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1371/journal.pgen.1002064$2DOI
100 1 $aPEDROSA, F. O.
245 $aGenome of Herbaspirillum seropedicae Strain SmR1, a specialized diazotrophic endophyte of tropical grasses.
260 $c2011
520 $aThe molecular mechanisms of plant recognition, colonization, and nutrient exchange between diazotrophic endophytes and plants are scarcely known. Herbaspirillum seropedicae is an endophytic bacterium capable of colonizing intercellular spaces of grasses such as rice and sugar cane. The genome of H. seropedicae strain SmR1 was sequenced and annotated by The Paraná State Genome Programme?GENOPAR. The genome is composed of a circular chromosome of 5,513,887 bp and contains a total of 4,804 genes. The genome sequence revealed that H. seropedicae is a highly versatile microorganism with capacity to metabolize a wide range of carbon and nitrogen sources and with possession of four distinct terminal oxidases. The genome contains a multitude of protein secretion systems, including type I, type II, type III, type V, and type VI secretion systems, and type IV pili, suggesting a high potential to interact with host plants. H. seropedicae is able to synthesize indole acetic acid as reflected by the four IAA biosynthetic pathways present. A gene coding for ACC deaminase, which may be involved in modulating the associated plant ethylene-signaling pathway, is also present. Genes for hemagglutinins/hemolysins/adhesins were found and may play a role in plant cell surface adhesion. These features may endow H. seropedicae with the ability to establish an endophytic life-style in a large number of plant species.
650 $aGenome
650 $aGrasses
650 $aHerbaspirillum seropedicae
650 $aNitrogen fixation
650 $aGenoma
650 $aGraminea tropical
653 $aFixação nitrogênio
700 1 $aMONTEIRO, R. A.
700 1 $aWASSEM, R.
700 1 $aCRUZ, L. M.
700 1 $aAYUB, R. A.
700 1 $aCOLAUTO, N. B.
700 1 $aFERNANDEZ, M. A.
700 1 $aFUNGARO, M. H. P.
700 1 $aGRISARD, E. C.
700 1 $aHUNGRIA, M.
700 1 $aMADEIRA, H. M. F.
700 1 $aNODARI, R. O.
700 1 $aOSAKU, C. A.
700 1 $aPETZL-ERLER, M. L.
700 1 $aTERENZI, H.
700 1 $aVIEIRA, L. G. E.
700 1 $aSTEFFENS, M. B. R.
700 1 $aWEISS, V. A.
700 1 $aPEREIRA, L. F. P.
700 1 $aALMEIDA, M. I. M.
700 1 $aALVES, L. R.
700 1 $aMARIN, A.
700 1 $aARAUJO, L. M.
700 1 $aBALSANELLI, E.
700 1 $aBAURA, V. A.
700 1 $aCHUBATSU, L. S.
700 1 $aFAORO, H.
700 1 $aFAVETTI, A.
700 1 $aFRIEDERMANN, G.
700 1 $aGLIENKE, C.
700 1 $aKARP, S.
700 1 $aKAVA-CORDEIRO, V.
700 1 $aRAITTZ, R. T.
700 1 $aRAMOS, H. J. O.
700 1 $aRIBEIRO, E. M. S. F.
700 1 $aRIGO, L. U.
700 1 $aROCHA, S. N.
700 1 $aSCHWAB, S.
700 1 $aSILVA, A. G.
700 1 $aSOUZA, E. M.
700 1 $aMICHELLE Z. TADRA-SFEIR
700 1 $aTORRES, R. A.
700 1 $aDABUL, A. N. G.
700 1 $aSOARES, M. A. M.
700 1 $aGASQUES, L. S.
700 1 $aGIMENES, C. C. T.
700 1 $aVALLE, J. S.
700 1 $aCIFERRI, R. R.
700 1 $aCORREA, L. C.
700 1 $aMURACE, N. K.
700 1 $aPAMPHILE, J. A.
700 1 $aPATUSSI, E. V.
700 1 $aPRIOLI, A. J.
700 1 $aPRIOLI, S. M. A.
700 1 $aROCHA, C. L. M. S. C.
700 1 $aARANTES, O. M. N.
700 1 $aFURLANETO, M. C.
700 1 $aGODOY, L. P.
700 1 $aOLIVEIRA, C. E. C.
700 1 $aSATORI, D.
700 1 $aVILAS-BOAS, L. A.
700 1 $aWATANABE, M. A. E.
700 1 $aDAMBROS, B. P.
700 1 $aGUERRA, M. P.
700 1 $aMATHIONI, S. M.
700 1 $aSANTOS, K. L.
700 1 $aSTEINDEL, M.
700 1 $aVERNAL, J.
700 1 $aBARCELLOS, F. G.
700 1 $aCAMPO, R. J.
700 1 $aCHUEIRE, L. M. O.
700 1 $aNICOLÁS, M. F.
700 1 $aPEREIRA-FERRARI, L.
700 1 $aSILVA, J. L. da C.
700 1 $aGIOPPO, N. M. R.
700 1 $aMARGARIDO, V. P.
700 1 $aMENCK-SOARES, M. A.
700 1 $aPINTO, F. G. S.
700 1 $aSIMÃO, R. de C. G.
700 1 $aTAKAHASHI, E. K.
700 1 $aYATES, M. G.
700 1 $aSOUZA, E. M.
773 $tPLoS Genetics$gv. 7, n. 5, p. 1-10, may 2011.
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