| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
14/10/2009 |
|
Data da última atualização: |
02/02/2010 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
GARCIA, S. A. L.; TALEBI, R.; FERREIRA, C. F.; VROH, I.; PAIVA, L. V.; SOUZA JUNIOR, M. T.; KEMA, G. H. J. |
|
Afiliação: |
S. A. L. Garcia, PRI/UFLA; R.T. ALEBI, PRI-Plant Research International; Cláudia Fortes Ferreira, CNPMF; I. VROH, IITA; Luciano Vilela Paiva, UFL; Manoel Teixeira Souza Junior, LABEX Europe; Gert H. J. Kema, PRI-Plant Research International. |
|
Título: |
Identification and validation of EST-derived Molecular markers, TRAP and VNTR, for banana research. |
|
Ano de publicação: |
2009 |
|
Fonte/Imprenta: |
In: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p. 10. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The advent of high-throughput sequencing technology has generated abundant information on DNA sequences for the genomes of many plant species. Expressed Sequence Tag (EST), a unique DNA sequence derived from a cDNA library and therefore representing a gene which has been transcribed in a specific tissue or at some stage of development , is one type of DNA sequence made highly available lately for many important crop species. Molecular markers are used for bridging DNA sequence information with particular phenotypes and are useful tools for genotyping germplasm collections and also for tagging genes governing desirable agronomic traits. In this sense, there is always a strong demand for better marker techniques to better utilise the existing sequence information. A transcriptome database from banana (Musa spp.), DATAMusa, containing 42,724 ESTs from 11 different cDNA libraries and encompassing approximately 24 Mb of DNA sequence, was used in this study for the design of primers to PCR amplify txo types of EST-derived molecular markers, Variable Nucleotide Tandem Repeat (VNTR) and Target Region Amplification Polymorphism (TRAP). These primers were then validated against a panel of 14 Musa diploid genotypes and produced 33 (VNTR) and 119 (TRAP) alleles. Used separately or together, both types of markers were able to discriminate Musa genotypes from different genome background (A or B genomes). The TRAP alleles identified derived from only one unigene, while the VNTR derived from 12 unigenes. Based on the results from this study, it can be said that EST-derived markers are an important source of polymorphism to be used in genetic diversity and gene discovery studies in banana. MenosThe advent of high-throughput sequencing technology has generated abundant information on DNA sequences for the genomes of many plant species. Expressed Sequence Tag (EST), a unique DNA sequence derived from a cDNA library and therefore representing a gene which has been transcribed in a specific tissue or at some stage of development , is one type of DNA sequence made highly available lately for many important crop species. Molecular markers are used for bridging DNA sequence information with particular phenotypes and are useful tools for genotyping germplasm collections and also for tagging genes governing desirable agronomic traits. In this sense, there is always a strong demand for better marker techniques to better utilise the existing sequence information. A transcriptome database from banana (Musa spp.), DATAMusa, containing 42,724 ESTs from 11 different cDNA libraries and encompassing approximately 24 Mb of DNA sequence, was used in this study for the design of primers to PCR amplify txo types of EST-derived molecular markers, Variable Nucleotide Tandem Repeat (VNTR) and Target Region Amplification Polymorphism (TRAP). These primers were then validated against a panel of 14 Musa diploid genotypes and produced 33 (VNTR) and 119 (TRAP) alleles. Used separately or together, both types of markers were able to discriminate Musa genotypes from different genome background (A or B genomes). The TRAP alleles identified derived from only one unigene, while the VNTR derived fr... Mostrar Tudo |
|
Thesagro: |
Banana; Marcador Molecular. |
|
Thesaurus Nal: |
Musa. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02450naa a2200229 a 4500
001 1656060
005 2010-02-02
008 2009 bl uuuu u00u1 u #d
100 1 $aGARCIA, S. A. L.
245 $aIdentification and validation of EST-derived Molecular markers, TRAP and VNTR, for banana research.
260 $c2009
520 $aThe advent of high-throughput sequencing technology has generated abundant information on DNA sequences for the genomes of many plant species. Expressed Sequence Tag (EST), a unique DNA sequence derived from a cDNA library and therefore representing a gene which has been transcribed in a specific tissue or at some stage of development , is one type of DNA sequence made highly available lately for many important crop species. Molecular markers are used for bridging DNA sequence information with particular phenotypes and are useful tools for genotyping germplasm collections and also for tagging genes governing desirable agronomic traits. In this sense, there is always a strong demand for better marker techniques to better utilise the existing sequence information. A transcriptome database from banana (Musa spp.), DATAMusa, containing 42,724 ESTs from 11 different cDNA libraries and encompassing approximately 24 Mb of DNA sequence, was used in this study for the design of primers to PCR amplify txo types of EST-derived molecular markers, Variable Nucleotide Tandem Repeat (VNTR) and Target Region Amplification Polymorphism (TRAP). These primers were then validated against a panel of 14 Musa diploid genotypes and produced 33 (VNTR) and 119 (TRAP) alleles. Used separately or together, both types of markers were able to discriminate Musa genotypes from different genome background (A or B genomes). The TRAP alleles identified derived from only one unigene, while the VNTR derived from 12 unigenes. Based on the results from this study, it can be said that EST-derived markers are an important source of polymorphism to be used in genetic diversity and gene discovery studies in banana.
650 $aMusa
650 $aBanana
650 $aMarcador Molecular
700 1 $aTALEBI, R.
700 1 $aFERREIRA, C. F.
700 1 $aVROH, I.
700 1 $aPAIVA, L. V.
700 1 $aSOUZA JUNIOR, M. T.
700 1 $aKEMA, G. H. J.
773 $tIn: ISHS/PROMUSA BANANA SYMPOSIUM, 2009, Guangzhou, China. Global perspectives on asian challenges: programme and abstracts. Guangzhou: ISHS: Promusa, 2009. p. 10.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Nenhum exemplar cadastrado para este documento. |
Voltar
|
|
|
| Registros recuperados : 69 | |
| 61. |  | COTTA, M. G.; BARROS, L. M. G.; ALMEIDA, J. D. de; SANTANA, R. H.; BARBOSA, E. A.; ALVES, G. S. C.; PAIVA, L. V.; CARNEIRO, M.; ANDRADE, A. C.; MARRACCINI, P. Lipid transfer proteins in coffee: isolation of a Coffea Orthologs, Coffea Arabica Homeologs, expression during coffee fruit development and promoter analysis in transgenic tobacco plants. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 2012, San José. Proceedings... [S.l]: Association for Science and Information on Coffee (ASIC), 2012. p. 231.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| 62. | | CARVALHO, M. H. de; ROSA, S. D. V. F. da; COELHO, S. V. B.; GUIMARÃES, C. C.; MARTINS, R. de S.; CLEMENTE, A. da C. S.; PAIVA, L. V. Drying of arabica coffee and its effect on the gene expression and activity of enzymes linked to seed physiological quality. Acta Scientiarum: Agronomy, v. 45, e56908, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Café. |
|  |
| 63. | | CAMPOS, M. A.; TARGON, M. L. P. N.; SOUZA, A. A.; MONGE, G. A.; TEIXEIRA, J. E. C.; TAKITA, M. A.; KISHI, L.; PAIVA, L. V.; MACHADO, M. A. Elicitin-like genes from Phytophthora parasitica regulated during the interaction with Cirtros sinensis 'Pêra IAC'. Fitopatologia Brasileira, Brasília, DF, v. 29, p. S276, ago. 2004. Edição dos Resumos do XXXVII Congresso Brasileiro de Fitopatologia, Gramado, RS, ago. 2004.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 64. | | CASARIN, T.; FREITAS, N. C.; PINTO, R. T.; BREITLER, J. C.; RODRIGUES, L. A. Z.; MARRACCINI, P.; ETIENNE, H.; DINIZ, L. E. C.; ANDRADE, A. C.; PAIVA, L. V. Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canéfora. Scientific Reports, v. 12, 17270, 2022. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
| |
| 65. | | CASARIN, T.; FREITAS, N. C.; PINTO, R. T.; BREITLER, J-C.; RODRIGUES, L. A. Z.; MARRACCINI, P.; ETIENNE, H.; DINIZ, L. E. C.; ANDRADE, A. C.; PAIVA, L. V. Multiplex CRISPR/Cas9-mediated knockout of the phytoene desaturase gene in Coffea canephora. Scientific Reports, v. 12, 17270, 2022. 10 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
| |
| 66. | | COELHO, G. T. C. P.; CARNEIRO, N. P.; KARTHIKEYAN, A. S.; RAGHOTHAMA, K. G.; SCHAFFERT, R. E.; BRANDAO, R. L.; PAIVA, L. V.; SOUZA, I. R. P. de; ALVES, V. M.; IMOLESI, A.; CARVALHO, C. H. S.; CARNEIRO, A. A. A phosphate transporter promoter from Arabidopsis thaliana AtPHT1;4 gene drives preferential gene expression in transgenic maize roots under phosphorus starvation. Plant Molecular Biology Reporter, Athens, v. 28, p. 717-723, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 67. | | CARNEIRO, A. A.; COELHO, G. T. C. P.; VASCONCELOS, F. V.; SOUZA, I. R. P. de; OLIVEIRA, E. de; PETRILLO, C. P.; PAIVA, L. V.; ARAGÃO, F. J. L.; CARNEIRO, N. P. Resistência ao Sugarcane mosaic virus (SCMV) por RNAi em plantas transgênicas de milho. In: CONGRESSO NACIONAL DE MILHO E SORGO, 27.; SIMPOSIO BRASILEIRO SOBRE A LAGARTA-DO-CARTUCHO, SPODOPTERA FRUGIPERDA, 3.; WORKSHOP SOBRE MANEJO E ETIOLOGIA DA MANCHA BRANCA DO MILHO, 2008, Londrina. Agroenergia, produção de alimentos e mudanças climáticas: desafios para milho e sorgo: trabalhos e palestras. [Londrina]: IAPAR; [Sete Lagoas]: Embrapa Milho e Sorgo, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 68. | | FARIA, M. V.; MALUF, W. R.; PAIVA, L. V.; CARDOSO, M. das G.; RESENDE, J. T. V. de; RESENDE, F. V. de; BENITES, F. R. G.; NASCIMENTO, I. R. do; MENEZES, C. B. de; NOGUEIRA, D. W.; GONÇALVES, L. D.; ANDRADE JÚNIOR, V. C. de; VARGAS, P. F. Pigmentos carotenóides em frutos de híbridos de tomateiros longa vida portadores dos alelos rin, alc, og e/ou heterozigose em diferentes backgrounds genéticos. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 364, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 69. | | RUIZ, J. C.; D'AFONSECA, V.; SILVA, A.; ALI, A.; PINTO, A. C.; SANTOS, A. R.; ROCHA, A. A. M. C.; LOPES, D. O.; DORELLA, F. A.; PACHECO, L. G. C.; COSTA, M. P.; TURK, M. Z.; SEYFFERT, N.; MORAES, P. M. R. O.; SOARES, S. C.; ALMEIDA, S. S.; CASTRO, T. L. P.; ABREU, V. A. C.; TROST, E.; BAUMBACH, J.; TAUCH, A.; SCHNEIDER, M. P. C.; McCULLOCH, J.; CERDEIRA, L. T.; RAMOS, R. T. J.; ZERLOTINI, A.; DOMINITINI, A.; RESENDE, D. M.; COSER, E. M.; OLIVEIRA, L. M.; PEDROSA, A. L.; VIEIRA, C. U.; GUIMARAES, C. T.; BARTHOLOMEU, D. C.; OLIVEIRA, D. M.; SANTOS, F. R.; RABELO, E. M.; LOBO, F. P.; FRANCO, G. R.; COSTA, A. F.; CASTRO, I. M.; DIAS, S. R. C.; FERRO, J. A.; ORTEGA, J. M.; PAIVA, L. V.; ALMEIDA, J. F.; GOULART, L. R.; FERRO, M. I. T.; CARNEIRO, N. P.; FALCÃO, P. R. K.; GRYNBERG, P.; TEIXEIRA, S. M. R.; BROMMONSCHENKEL, S.; OLIVEIRA, S. C.; MEYER, R.; MOORE, R. J.; MIYOSHI, A.; OLIVEIRA, G. C.; AZEVEDO, V. Evidence for reductive genome evolution and lateral acquisition of virulence functions in two Corynebacterium pseudotuberculosis strains. Plos One, San Francisco, v. 6, n. 4, p. 1-16, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Milho e Sorgo. |
| |
| Registros recuperados : 69 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
