Data/hora: 21/09/2024 12:19:45

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	18/03/2016	
Data da última atualização: 	14/02/2018	
Autoria: 	GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L.	
Afiliação: 	JULIANA MARCOLINO GOMES, UEL; THIAGO J. NAKAYAMA, UFV; RAFAELA RIBEIRO REIS, UEL; FABIANA APARECIDA RODRIGUES; LILIANE MARCIA MERTZ HENNING, CNPSO; RENATA FUGANTI PAGLIARINI; HUGO BRUNO CORREA MOLINARI, CNPAE; FRANK HARMON, USDA; ALEXANDRE LIMA NEPOMUCENO, SRI.	
Título: 	Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress.	
Ano de publicação: 	2015	
Fonte/Imprenta: 	In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego.	
Páginas: 	PO 203	
Idioma: 	Inglês	
Conteúdo: 	Drought is the major limiting factor influencing productivity of commercially important crops. In the past several years, drought has severely affected soybean areas worldwide, and climate change sceneries suggest increase of drought stress episodes. The circadian clock drives diurnal and seasonal oscillations in organisms, enhancing fitness and could play an important role in drought responses. Because little is known about the relations between the circadian clock and drought stress, we used RT-qPCR and RNA-seq to assess the gene expression profiles in response to drought along the day. Soybean plants were analyzed under moderate and severe drought stress conditions, sampling with 4-hour intervals during 24h. The resultant gene expression database allowed us to select candidate genes for functional analysis by overexpression in Arabidopsis. Plants were transformed by the floral dip method and transformants were selected in MS medium containing the selection marker hygromycin. The transgene presence was checked by PCR with specific primers. Drought and osmotic stress experiments were performed with one-copy homozygous lines. Our results show that the overexpression of a soybean gene encoding a stress-responsive A/B Barrel Domain protein decreased germination velocity in response to different PEG concentrations, suggesting an increase of osmotic stress sensitivity. On the contrary, the overexpression of a gene coding a yet uncharacterized-protein increased transgenic plants survival in response to drought. Together our results suggest that we identified key genes in drought tolerance. Further studies of the transgenic plants will help to unveil the drought tolerance mechanisms, contributing to the development of drought tolerant soybeans.	
Thesagro: 	Gene; Resistência à seca; Soja.	
Thesaurus Nal: 	Soybeans; Water stress.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/141414/1/Gene-expression-and-functional-...-2015.html	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (36662-1) - UPC - RA - DD -  -  -  - DIS