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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
18/11/2013 |
Data da última atualização: |
21/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. A.; VIEIRA-da-MOTTA, O. |
Afiliação: |
RITA DE CÁSSIA DA SILVA PAES; UBIRATAN PIOVEZAN, CPAP. |
Título: |
Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Veterinary Microbiology, v. 165, p. 448-454, 2013. |
ISSN: |
0378-1135 |
Idioma: |
Inglês |
Conteúdo: |
The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil. MenosThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the we... Mostrar Tudo |
Palavras-Chave: |
Feral swine; Glycoprotein; PCR; Seroneutralization assay. |
Thesagro: |
Elisa. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/92652/1/1-s2.0-S0378113513002009-main.pdf
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Marc: |
LEADER 02356naa a2200277 a 4500 001 1971371 005 2017-07-21 008 2013 bl uuuu u00u1 u #d 022 $a0378-1135 100 1 $aPAES, R. de C. da S. 245 $aSerological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. 260 $c2013 520 $aThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil. 650 $aElisa 653 $aFeral swine 653 $aGlycoprotein 653 $aPCR 653 $aSeroneutralization assay 700 1 $aFONSECA JUNIOR, A. A. 700 1 $aMONTEIRO, L. A. R. C. 700 1 $aJARDIM, G. C. 700 1 $aPIOVEZAN, U. 700 1 $aHERRERA, H. M. 700 1 $aMAURO, R. A. 700 1 $aVIEIRA-da-MOTTA, O. 773 $tVeterinary Microbiology$gv. 165, p. 448-454, 2013.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
11/01/2008 |
Data da última atualização: |
18/09/2013 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
CONSOLIN FILHO, N.; LEITE, F. de L.; CARVALHO, E. R.; VENÂNCIO, E. C.; VAZ, V. M. P.; MATTOSO, L. H. C. |
Afiliação: |
Carlos Manoel Pedro Vaz - Embrapa Instrumentação Agropecuária. Luiz Henrique Capparelli Mattoso - Embrapa Instrumentação Agropecuária. |
Título: |
Study of poly(o-ethoxyaniline) interactions with herbicides and evaluation of conductive polymer potential used in electrochemical sensors. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
Journal of the Brazilian Chemical Society, São Paulo, v. 18, n. 3, p. 577-584, 2007. |
Idioma: |
Inglês |
Palavras-Chave: |
Herbicidas; Interação; Polímeros condutores; Sensores. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/89728/1/Proci-07.00176.PDF
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Marc: |
LEADER 00715naa a2200217 a 4500 001 1025356 005 2013-09-18 008 2007 bl uuuu u00u1 u #d 100 1 $aCONSOLIN FILHO, N. 245 $aStudy of poly(o-ethoxyaniline) interactions with herbicides and evaluation of conductive polymer potential used in electrochemical sensors. 260 $c2007 653 $aHerbicidas 653 $aInteração 653 $aPolímeros condutores 653 $aSensores 700 1 $aLEITE, F. de L. 700 1 $aCARVALHO, E. R. 700 1 $aVENÂNCIO, E. C. 700 1 $aVAZ, V. M. P. 700 1 $aMATTOSO, L. H. C. 773 $tJournal of the Brazilian Chemical Society, São Paulo$gv. 18, n. 3, p. 577-584, 2007.
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Embrapa Instrumentação (CNPDIA) |
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