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Registro Completo |
Biblioteca(s): |
Embrapa Pantanal. |
Data corrente: |
18/11/2013 |
Data da última atualização: |
21/07/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. A.; VIEIRA-da-MOTTA, O. |
Afiliação: |
RITA DE CÁSSIA DA SILVA PAES; UBIRATAN PIOVEZAN, CPAP. |
Título: |
Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Veterinary Microbiology, v. 165, p. 448-454, 2013. |
ISSN: |
0378-1135 |
Idioma: |
Inglês |
Conteúdo: |
The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil. MenosThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the we... Mostrar Tudo |
Palavras-Chave: |
Feral swine; Glycoprotein; PCR; Seroneutralization assay. |
Thesagro: |
Elisa. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/92652/1/1-s2.0-S0378113513002009-main.pdf
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Marc: |
LEADER 02356naa a2200277 a 4500 001 1971371 005 2017-07-21 008 2013 bl uuuu u00u1 u #d 022 $a0378-1135 100 1 $aPAES, R. de C. da S. 245 $aSerological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. 260 $c2013 520 $aThe feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky?s disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil. 650 $aElisa 653 $aFeral swine 653 $aGlycoprotein 653 $aPCR 653 $aSeroneutralization assay 700 1 $aFONSECA JUNIOR, A. A. 700 1 $aMONTEIRO, L. A. R. C. 700 1 $aJARDIM, G. C. 700 1 $aPIOVEZAN, U. 700 1 $aHERRERA, H. M. 700 1 $aMAURO, R. A. 700 1 $aVIEIRA-da-MOTTA, O. 773 $tVeterinary Microbiology$gv. 165, p. 448-454, 2013.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registros recuperados : 5 | |
2. | | KNACKFUSS, F. B.; PAES, R. de C. da S.; MENEZES, A. N. de; HERRERA, H. M.; PIOVEZAN, U.; BONVIVINO, C. R. Análise filogenética entre Suínos Asselvajados no Pantanal e comerciais (Sus scrofa, Artiodactyla: Suidae). Boletim da Sociedade Brasileira de Mastozoologia, v. 67, p. 39-45, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Pantanal. |
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3. | | BRAZ, P. H.; JULIANO, R. S.; LYRIO, R. C.; PAES, R. de C. da S.; LEAL FILHO, J. M.; PELLEGRIN, A. O. Soroprevalência de brucelose em suínos de granjas comerciais e em criatórios de subsistência. Revista Brasileira de Ciência Veterinária, v. 28, n. 3, p. 162-166, jul./set.2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Pantanal. |
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4. | | PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. de A.; VIEIRA-DA-MOTA, O. Serological and molecular investigation of the prevalence of Aujeszky?s disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. Veterinary Microbiology, v.165, n. 3-4, p. 448-454, 2013 Short communicationTipo: Nota Técnica/Nota Científica |
Biblioteca(s): Embrapa Gado de Corte. |
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5. | | PAES, R. de C. da S.; FONSECA JUNIOR, A. A.; MONTEIRO, L. A. R. C.; JARDIM, G. C.; PIOVEZAN, U.; HERRERA, H. M.; MAURO, R. A.; VIEIRA-da-MOTTA, O. Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil. Veterinary Microbiology, v. 165, p. 448-454, 2013.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Pantanal. |
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Registros recuperados : 5 | |
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