Data/hora: 26/09/2024 10:29:10

Biblioteca(s): 	Embrapa Agroenergia; Embrapa Agroindústria de Alimentos.	
Data corrente: 	04/03/2022	
Data da última atualização: 	10/10/2022	
Autoria: 	SANTOS, A. DA S.; PEREIRA, G. M.; ALMEIDA, A. M. M. DE; SANTOS, R. R. DOS; TERZI, S. da C.; PACHECO, T. F.; VERMELHO, A. B.; CABRAL, L. M. C.; COURI, S.; DAMASO, M. C. T.	
Afiliação: 	ALEX DA SILVA SANTOS, Univesidade Federal Rural do Rio de Janeiro - UFRRJ; GABRIELA MOYSES PEREIRA, Universidade Federal do Rio de Janeiro - UFRJ; ANA MARIA MAZOTTO DE ALMEIDA, Universidade Federal do Rio de Janeiro - UFRJ; REGIANE RIBEIRO DOS SANTOS, Universidade Federal Rural do Rio de Janeiro - UFRRJ; SELMA DA COSTA TERZI, CTAA; THALYTA FRAGA PACHECO, Universidade Federal do Rio de Janeiro - UFRJ; ALANE BEATRIZ VERMELHO, Universidade Federal do Rio de Janeiro - UFRJ; LOURDES MARIA CORREA CABRAL, CTAA; SONIA COURI, CTAA; MONICA CARAMEZ TRICHES DAMASO, CNPAE.	
Título: 	Production, concentration and partial characterization of an enzymatic extract produced by an Aspergillus niger mutant in solid state fermentation.	
Ano de publicação: 	2022	
Fonte/Imprenta: 	Preparative Biochemistry & Biotechnology, 2022.	
DOI: 	https://doi.org/10.1080/10826068.2022.2033989	
Idioma: 	Inglês	
Notas: 	Early Access.	
Conteúdo: 	An enzymatic extract from Aspergillus niger 3T5B8 was produced by Solid State Fermentation (SSF) in aerated columns, using wheat bran as substrate. A combination of extracts produced using three different process conditions varying temperature, pH and aeration formed the final extract (Mixture). The Mixture was concentrated by an ultrafiltration process that partially purified and provided an efficient recovery of the enzymatic activities of xylanase (88.89%), polygalacturonase (89.3%), beta-glucosidase (93.15%), protease (98.68%) and carboxymethylcellulase (CMCase) (98.93%). SDS-PAGE analysis showed 15 visible protein bands in the crude and concentrated Mixture with molecular weights ranging from 15.1 to 104.6 kDa. Thin layer chromatography confirmed the effective action of beta-glucosidase and xylanase hydrolysis activities over cellobiose and xylan, respectively. A central composite design (CCD) with two variables and four replicates at the center points was used to determine the optimal temperature and pH for CMCase and beta-glucosidase. The optimal temperature was 78.9 degrees C and pH 3.8 for CMCase and 52.8 degrees C and pH 4.8 for beta-glucosidase, respectively.	
Palavras-Chave: 	Aspergillus niger 3T5B8; Cellulolytic enzymes; Enzyme concentration; Partial characterization.	
Thesagro: 	Aspergillus Niger.	
Thesaurus Nal: 	Enzymes; Solid state fermentation; Ultrafiltration.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Agroindústria de Alimentos (CTAA)	
Exemplares: 	CTAA - (15297-1) - UPC - AP - DD -  -  -  - DIS
CNPAE - (4017-1) - UPC - AP - DD -  -  -  - DIS