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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
11/04/2001 |
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Data da última atualização: |
11/04/2001 |
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Autoria: |
GIANINAZZI-PEARSON, V.; ARNOULD, C.; OUFATTOLE, M.; ARANGO, M.; GIANINAZZI, S. |
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Título: |
Differential activation of H+ _ATPase genes by an arbuscular mycorrhizal fungus in root cells of transgenic tobacco. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Panta, v.2 11, p.609-613, 2000. |
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Idioma: |
Inglês |
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Conteúdo: |
In arbuscular mycorrhizas, H+ -ATPase is active in the plant membrane around arbuscules but absent from plant mutants defective in arbuscule development (Gianinazzi-Pearson et al. 1995, Can. J. Bot. 73:S526-S532). The proton-pumping H+ -ATPase is encoded by a family of genes in plants. Immunocytochemical studies and promoter-gus A fusion assays were performed in transgenic tobacco (Nicotiana tabacum L.) to determine whether the periarbuscular enzyme activity results from de-novo activation of plant genes by an arbuscular mycorrhizal fungus. The H+ -ATPase protein was localized in the plant membrane around arbuscule hyphae. The enzyme was absent from non-colonized cortical cells. Regulation of seven H+ -ATPase genes (pma) was compared in non-mycorrhizal and mycorrhizal roots by histochemical detection of B-glucuronidase (GUS) activity. Two genes (pma2, pma4) were induced in arbuscule-containing cells of mycorrhizal roots but not in non-mycorrhizal cortical tissues or senescent mycorrhiza. It is concluded that de-novo H+ -ATPase activity in the periarbuscular membrane results from selective induction of two H+ -ATPase genes, which can have diverse roles in plant-fungal interactions at the symbiotic interface. |
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Thesagro: |
Fumo; Fungo; Micorriza Vesicular Arbuscular; Raiz; Simbiose. |
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Thesaurus Nal: |
fungi; roots; symbiosis; tobacco; vesicular arbuscular mycorrhizae. |
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Categoria do assunto: |
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Marc: |
LEADER 02001naa a2200289 a 4500
001 1621199
005 2001-04-11
008 2000 bl --- 0-- u #d
100 1 $aGIANINAZZI-PEARSON, V.
245 $aDifferential activation of H+ _ATPase genes by an arbuscular mycorrhizal fungus in root cells of transgenic tobacco.
260 $c2000
520 $aIn arbuscular mycorrhizas, H+ -ATPase is active in the plant membrane around arbuscules but absent from plant mutants defective in arbuscule development (Gianinazzi-Pearson et al. 1995, Can. J. Bot. 73:S526-S532). The proton-pumping H+ -ATPase is encoded by a family of genes in plants. Immunocytochemical studies and promoter-gus A fusion assays were performed in transgenic tobacco (Nicotiana tabacum L.) to determine whether the periarbuscular enzyme activity results from de-novo activation of plant genes by an arbuscular mycorrhizal fungus. The H+ -ATPase protein was localized in the plant membrane around arbuscule hyphae. The enzyme was absent from non-colonized cortical cells. Regulation of seven H+ -ATPase genes (pma) was compared in non-mycorrhizal and mycorrhizal roots by histochemical detection of B-glucuronidase (GUS) activity. Two genes (pma2, pma4) were induced in arbuscule-containing cells of mycorrhizal roots but not in non-mycorrhizal cortical tissues or senescent mycorrhiza. It is concluded that de-novo H+ -ATPase activity in the periarbuscular membrane results from selective induction of two H+ -ATPase genes, which can have diverse roles in plant-fungal interactions at the symbiotic interface.
650 $afungi
650 $aroots
650 $asymbiosis
650 $atobacco
650 $avesicular arbuscular mycorrhizae
650 $aFumo
650 $aFungo
650 $aMicorriza Vesicular Arbuscular
650 $aRaiz
650 $aSimbiose
700 1 $aARNOULD, C.
700 1 $aOUFATTOLE, M.
700 1 $aARANGO, M.
700 1 $aGIANINAZZI, S.
773 $tPanta$gv.2 11, p.609-613, 2000.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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